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The relationship of micronucleus frequency and nuclear division index with coronary artery disease SYNTAX and Gensini scores
OBJECTIVE: We aimed to evaluate the relationship of micronucleus (MN) frequency and nuclear division index (NDI) with SYNTAX and Gensini scores and thrombolysis in myocardial infarction (TIMI) frame counts of coronary arteries in patients undergoing coronary angiography. METHODS: In a single-center...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Kare Publishing
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5477082/ https://www.ncbi.nlm.nih.gov/pubmed/28315571 http://dx.doi.org/10.14744/AnatolJCardiol.2017.7582 |
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author | İpek, Emrah Ermiş, Emrah Uysal, Handan Kızılet, Halit Demirelli, Selami Yıldırım, Erkan Ünver, Sedat Demir, Bircan Kızılet, Nergiz |
author_facet | İpek, Emrah Ermiş, Emrah Uysal, Handan Kızılet, Halit Demirelli, Selami Yıldırım, Erkan Ünver, Sedat Demir, Bircan Kızılet, Nergiz |
author_sort | İpek, Emrah |
collection | PubMed |
description | OBJECTIVE: We aimed to evaluate the relationship of micronucleus (MN) frequency and nuclear division index (NDI) with SYNTAX and Gensini scores and thrombolysis in myocardial infarction (TIMI) frame counts of coronary arteries in patients undergoing coronary angiography. METHODS: In a single-center prospective observational study, a total of 63 individuals, 48 consecutive patients with coronary artery disease (CAD) and 15 healthy people were included. Before coronary angiography (exposure to X-ray), blood samples were collected for lymphocyte cultures, MN and NDI measurements. According to the SYNTAX and Gensini scores, patients were allocated into two groups. Group 1 and 2 included the patients with SYNTAX scores <22 and ≥22 points, respectively. Similarly, groups according to Gensini scores included the ones <23 and ≥23 points. MN test was used for in vitro studies in human peripheral lymphocytes. Binucleated lymphocytes were calculated for each patient. RESULTS: MN frequency was significantly higher in group 2 than group 1 and in group 1 than control group (p<0.001). NDI was significantly higher in control group than group 1 and in group 1 than group 2 (p=0.003). MN frequency had positive but moderate correlation with SYNTAX and Gensini scores and TFCs of left anterior descending (LAD), circumflex and right coronary arteries (r=0.394, p=0.003; r=0.458, p<0.001; r=0.425, p<0.001; r=0.469, p<0.001; and r=0.475, p<0.001, respectively). CONCLUSION: We can conclude that as the degree of atherosclerosis increases and coronary flow worsens, MN frequency increases and NDI decreases. Our results may help to elucidate the relationship of DNA damage in pathophysiology of atherosclerosis and endothelial dysfunction in patients with stable CAD. |
format | Online Article Text |
id | pubmed-5477082 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Kare Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-54770822017-06-28 The relationship of micronucleus frequency and nuclear division index with coronary artery disease SYNTAX and Gensini scores İpek, Emrah Ermiş, Emrah Uysal, Handan Kızılet, Halit Demirelli, Selami Yıldırım, Erkan Ünver, Sedat Demir, Bircan Kızılet, Nergiz Anatol J Cardiol Original Investigation OBJECTIVE: We aimed to evaluate the relationship of micronucleus (MN) frequency and nuclear division index (NDI) with SYNTAX and Gensini scores and thrombolysis in myocardial infarction (TIMI) frame counts of coronary arteries in patients undergoing coronary angiography. METHODS: In a single-center prospective observational study, a total of 63 individuals, 48 consecutive patients with coronary artery disease (CAD) and 15 healthy people were included. Before coronary angiography (exposure to X-ray), blood samples were collected for lymphocyte cultures, MN and NDI measurements. According to the SYNTAX and Gensini scores, patients were allocated into two groups. Group 1 and 2 included the patients with SYNTAX scores <22 and ≥22 points, respectively. Similarly, groups according to Gensini scores included the ones <23 and ≥23 points. MN test was used for in vitro studies in human peripheral lymphocytes. Binucleated lymphocytes were calculated for each patient. RESULTS: MN frequency was significantly higher in group 2 than group 1 and in group 1 than control group (p<0.001). NDI was significantly higher in control group than group 1 and in group 1 than group 2 (p=0.003). MN frequency had positive but moderate correlation with SYNTAX and Gensini scores and TFCs of left anterior descending (LAD), circumflex and right coronary arteries (r=0.394, p=0.003; r=0.458, p<0.001; r=0.425, p<0.001; r=0.469, p<0.001; and r=0.475, p<0.001, respectively). CONCLUSION: We can conclude that as the degree of atherosclerosis increases and coronary flow worsens, MN frequency increases and NDI decreases. Our results may help to elucidate the relationship of DNA damage in pathophysiology of atherosclerosis and endothelial dysfunction in patients with stable CAD. Kare Publishing 2017-06 2017-03-09 /pmc/articles/PMC5477082/ /pubmed/28315571 http://dx.doi.org/10.14744/AnatolJCardiol.2017.7582 Text en Copyright: © 2017 Turkish Society of Cardiology http://creativecommons.org/licenses/by-nc-sa/4.0 This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License |
spellingShingle | Original Investigation İpek, Emrah Ermiş, Emrah Uysal, Handan Kızılet, Halit Demirelli, Selami Yıldırım, Erkan Ünver, Sedat Demir, Bircan Kızılet, Nergiz The relationship of micronucleus frequency and nuclear division index with coronary artery disease SYNTAX and Gensini scores |
title | The relationship of micronucleus frequency and nuclear division index with coronary artery disease SYNTAX and Gensini scores |
title_full | The relationship of micronucleus frequency and nuclear division index with coronary artery disease SYNTAX and Gensini scores |
title_fullStr | The relationship of micronucleus frequency and nuclear division index with coronary artery disease SYNTAX and Gensini scores |
title_full_unstemmed | The relationship of micronucleus frequency and nuclear division index with coronary artery disease SYNTAX and Gensini scores |
title_short | The relationship of micronucleus frequency and nuclear division index with coronary artery disease SYNTAX and Gensini scores |
title_sort | relationship of micronucleus frequency and nuclear division index with coronary artery disease syntax and gensini scores |
topic | Original Investigation |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5477082/ https://www.ncbi.nlm.nih.gov/pubmed/28315571 http://dx.doi.org/10.14744/AnatolJCardiol.2017.7582 |
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