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Single-cell transcriptome of early embryos and cultured embryonic stem cells of cynomolgus monkeys

In mammals, the development of pluripotency and specification of primordial germ cells (PGCs) have been studied predominantly using mice as a model organism. However, divergences among mammalian species for such processes have begun to be recognized. Between humans and mice, pre-implantation develop...

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Autores principales: Nakamura, Tomonori, Yabuta, Yukihiro, Okamoto, Ikuhiro, Sasaki, Kotaro, Iwatani, Chizuru, Tsuchiya, Hideaki, Saitou, Mitinori
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5477564/
https://www.ncbi.nlm.nih.gov/pubmed/28649393
http://dx.doi.org/10.1038/sdata.2017.67
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author Nakamura, Tomonori
Yabuta, Yukihiro
Okamoto, Ikuhiro
Sasaki, Kotaro
Iwatani, Chizuru
Tsuchiya, Hideaki
Saitou, Mitinori
author_facet Nakamura, Tomonori
Yabuta, Yukihiro
Okamoto, Ikuhiro
Sasaki, Kotaro
Iwatani, Chizuru
Tsuchiya, Hideaki
Saitou, Mitinori
author_sort Nakamura, Tomonori
collection PubMed
description In mammals, the development of pluripotency and specification of primordial germ cells (PGCs) have been studied predominantly using mice as a model organism. However, divergences among mammalian species for such processes have begun to be recognized. Between humans and mice, pre-implantation development appears relatively similar, but the manner and morphology of post-implantation development are significantly different. Nevertheless, the embryogenesis just after implantation in primates, including the specification of PGCs, has been unexplored due to the difficulties in analyzing the embryos at relevant developmental stages. Here, we present a comprehensive single-cell transcriptome dataset of pre- and early post-implantation embryo cells, PGCs and embryonic stem cells (ESCs) of cynomolgus monkeys as a model of higher primates. The identities of each transcriptome were also validated rigorously by other way such as immunofluorescent analysis. The information reported here will serve as a foundation for our understanding of a wide range of processes in the developmental biology of primates, including humans.
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spelling pubmed-54775642017-06-23 Single-cell transcriptome of early embryos and cultured embryonic stem cells of cynomolgus monkeys Nakamura, Tomonori Yabuta, Yukihiro Okamoto, Ikuhiro Sasaki, Kotaro Iwatani, Chizuru Tsuchiya, Hideaki Saitou, Mitinori Sci Data Data Descriptor In mammals, the development of pluripotency and specification of primordial germ cells (PGCs) have been studied predominantly using mice as a model organism. However, divergences among mammalian species for such processes have begun to be recognized. Between humans and mice, pre-implantation development appears relatively similar, but the manner and morphology of post-implantation development are significantly different. Nevertheless, the embryogenesis just after implantation in primates, including the specification of PGCs, has been unexplored due to the difficulties in analyzing the embryos at relevant developmental stages. Here, we present a comprehensive single-cell transcriptome dataset of pre- and early post-implantation embryo cells, PGCs and embryonic stem cells (ESCs) of cynomolgus monkeys as a model of higher primates. The identities of each transcriptome were also validated rigorously by other way such as immunofluorescent analysis. The information reported here will serve as a foundation for our understanding of a wide range of processes in the developmental biology of primates, including humans. Nature Publishing Group 2017-06-20 /pmc/articles/PMC5477564/ /pubmed/28649393 http://dx.doi.org/10.1038/sdata.2017.67 Text en Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0 This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the metadata files made available in this article.
spellingShingle Data Descriptor
Nakamura, Tomonori
Yabuta, Yukihiro
Okamoto, Ikuhiro
Sasaki, Kotaro
Iwatani, Chizuru
Tsuchiya, Hideaki
Saitou, Mitinori
Single-cell transcriptome of early embryos and cultured embryonic stem cells of cynomolgus monkeys
title Single-cell transcriptome of early embryos and cultured embryonic stem cells of cynomolgus monkeys
title_full Single-cell transcriptome of early embryos and cultured embryonic stem cells of cynomolgus monkeys
title_fullStr Single-cell transcriptome of early embryos and cultured embryonic stem cells of cynomolgus monkeys
title_full_unstemmed Single-cell transcriptome of early embryos and cultured embryonic stem cells of cynomolgus monkeys
title_short Single-cell transcriptome of early embryos and cultured embryonic stem cells of cynomolgus monkeys
title_sort single-cell transcriptome of early embryos and cultured embryonic stem cells of cynomolgus monkeys
topic Data Descriptor
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5477564/
https://www.ncbi.nlm.nih.gov/pubmed/28649393
http://dx.doi.org/10.1038/sdata.2017.67
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