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Comparison of single or multiple intratracheal administration for pulmonary toxic responses of nickel oxide nanoparticles in rats
OBJECTIVES: In this study, we focused on the qualitative and quantitative differences of the lung lesions induced by single or multiple intratracheal administration of nickel oxide nanoparticles (NiO). METHODS: Male rats were randomized into groups receiving intratracheal administrations in a single...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Japan Society for Occupational Health
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5478522/ https://www.ncbi.nlm.nih.gov/pubmed/27980250 |
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author | Senoh, Hideki Kano, Hirokazu Suzuki, Masaaki Ohnishi, Makoto Kondo, Hitomi Takanobu, Kenji Umeda, Yumi Aiso, Shigetoshi Fukushima, Shoji |
author_facet | Senoh, Hideki Kano, Hirokazu Suzuki, Masaaki Ohnishi, Makoto Kondo, Hitomi Takanobu, Kenji Umeda, Yumi Aiso, Shigetoshi Fukushima, Shoji |
author_sort | Senoh, Hideki |
collection | PubMed |
description | OBJECTIVES: In this study, we focused on the qualitative and quantitative differences of the lung lesions induced by single or multiple intratracheal administration of nickel oxide nanoparticles (NiO). METHODS: Male rats were randomized into groups receiving intratracheal administrations in a single dose or two to four divided doses of 2 mg/kg/bw. Bronchoalveolar lavage fluid (BALF) analyses were performed at 3 and 28 d post-dose. Histopathological analyses were performed at 28 and 91 d post-dose. RESULTS: BALF analyses revealed pulmonary injury, inflammation, and increases in the parameters indicating processing the foreign material in all the NiO-treated groups. Histopathological analyses showed the phagocytosis of NiO by alveolar macrophages, degeneration and necrosis of alveolar macrophages, and inflammatory responses. In the comparison between single and multiple administrations, the trend for stronger toxicity effects was observed after multiple application at 3 d post-dose, while the obvious toxicity effects were also seen in case of single administration. No particular differences of lung lesions depending on the frequency of administration at 28 and 91 d post-dose were evident. CONCLUSION: Intratracheal NiO administration induced strong toxic response thoroughly even by single administration. Therefore, single administration was concluded to be applicable to assess the inhalation toxicity of nanomaterials and can be used in the screening test. |
format | Online Article Text |
id | pubmed-5478522 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Japan Society for Occupational Health |
record_format | MEDLINE/PubMed |
spelling | pubmed-54785222017-06-29 Comparison of single or multiple intratracheal administration for pulmonary toxic responses of nickel oxide nanoparticles in rats Senoh, Hideki Kano, Hirokazu Suzuki, Masaaki Ohnishi, Makoto Kondo, Hitomi Takanobu, Kenji Umeda, Yumi Aiso, Shigetoshi Fukushima, Shoji J Occup Health Original OBJECTIVES: In this study, we focused on the qualitative and quantitative differences of the lung lesions induced by single or multiple intratracheal administration of nickel oxide nanoparticles (NiO). METHODS: Male rats were randomized into groups receiving intratracheal administrations in a single dose or two to four divided doses of 2 mg/kg/bw. Bronchoalveolar lavage fluid (BALF) analyses were performed at 3 and 28 d post-dose. Histopathological analyses were performed at 28 and 91 d post-dose. RESULTS: BALF analyses revealed pulmonary injury, inflammation, and increases in the parameters indicating processing the foreign material in all the NiO-treated groups. Histopathological analyses showed the phagocytosis of NiO by alveolar macrophages, degeneration and necrosis of alveolar macrophages, and inflammatory responses. In the comparison between single and multiple administrations, the trend for stronger toxicity effects was observed after multiple application at 3 d post-dose, while the obvious toxicity effects were also seen in case of single administration. No particular differences of lung lesions depending on the frequency of administration at 28 and 91 d post-dose were evident. CONCLUSION: Intratracheal NiO administration induced strong toxic response thoroughly even by single administration. Therefore, single administration was concluded to be applicable to assess the inhalation toxicity of nanomaterials and can be used in the screening test. Japan Society for Occupational Health 2016-12-15 2017-03-20 /pmc/articles/PMC5478522/ /pubmed/27980250 Text en https://creativecommons.org/licenses/by-nc-sa/4.0/ Journal of Occupational Health is an Open Access article distributed under the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. To view the details of this license, please visit (https://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Original Senoh, Hideki Kano, Hirokazu Suzuki, Masaaki Ohnishi, Makoto Kondo, Hitomi Takanobu, Kenji Umeda, Yumi Aiso, Shigetoshi Fukushima, Shoji Comparison of single or multiple intratracheal administration for pulmonary toxic responses of nickel oxide nanoparticles in rats |
title | Comparison of single or multiple intratracheal administration for pulmonary toxic responses of nickel oxide nanoparticles in rats |
title_full | Comparison of single or multiple intratracheal administration for pulmonary toxic responses of nickel oxide nanoparticles in rats |
title_fullStr | Comparison of single or multiple intratracheal administration for pulmonary toxic responses of nickel oxide nanoparticles in rats |
title_full_unstemmed | Comparison of single or multiple intratracheal administration for pulmonary toxic responses of nickel oxide nanoparticles in rats |
title_short | Comparison of single or multiple intratracheal administration for pulmonary toxic responses of nickel oxide nanoparticles in rats |
title_sort | comparison of single or multiple intratracheal administration for pulmonary toxic responses of nickel oxide nanoparticles in rats |
topic | Original |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5478522/ https://www.ncbi.nlm.nih.gov/pubmed/27980250 |
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