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Preventive Effect of Lactobacillus helveticus SBT2171 on Collagen-Induced Arthritis in Mice

We recently reported that the intraperitoneal inoculation of Lactobacillus helveticus SBT2171 inhibited the development of collagen-induced arthritis (CIA), a murine model of rheumatoid arthritis (RA). In the present study, we evaluated the effect of the oral administration of L. helveticus SBT2171...

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Autores principales: Yamashita, Maya, Matsumoto, Kurumi, Endo, Tsutomu, Ukibe, Ken, Hosoya, Tomohiro, Matsubara, Yumi, Nakagawa, Hisako, Sakai, Fumihiko, Miyazaki, Tadaaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5478730/
https://www.ncbi.nlm.nih.gov/pubmed/28680422
http://dx.doi.org/10.3389/fmicb.2017.01159
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author Yamashita, Maya
Matsumoto, Kurumi
Endo, Tsutomu
Ukibe, Ken
Hosoya, Tomohiro
Matsubara, Yumi
Nakagawa, Hisako
Sakai, Fumihiko
Miyazaki, Tadaaki
author_facet Yamashita, Maya
Matsumoto, Kurumi
Endo, Tsutomu
Ukibe, Ken
Hosoya, Tomohiro
Matsubara, Yumi
Nakagawa, Hisako
Sakai, Fumihiko
Miyazaki, Tadaaki
author_sort Yamashita, Maya
collection PubMed
description We recently reported that the intraperitoneal inoculation of Lactobacillus helveticus SBT2171 inhibited the development of collagen-induced arthritis (CIA), a murine model of rheumatoid arthritis (RA). In the present study, we evaluated the effect of the oral administration of L. helveticus SBT2171 on CIA development and on the regulation of antigen-specific antibody production and inflammatory immune cells, which have been implicated in the development of RA. Both oral administration and intraperitoneal inoculation of L. helveticus SBT2171 reduced joint swelling, body weight loss, and the serum level of bovine type II collagen (CII)-specific antibodies in the CIA mouse model. The intraperitoneal inoculation also decreased the arthritis incidence, joint damage, and serum level of interleukin (IL)-6. In addition, the numbers of total immune cells, total B cells, germinal center B cells, and CD4(+) T cells in the draining lymph nodes were decreased following intraperitoneal inoculation of L. helveticus SBT2171. These findings demonstrate the ability of L. helveticus SBT2171 to downregulate the abundance of immune cells and the subsequent production of CII-specific antibodies and IL-6, thereby suppressing the CIA symptoms, indicating its potential for use in the prevention of RA.
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spelling pubmed-54787302017-07-05 Preventive Effect of Lactobacillus helveticus SBT2171 on Collagen-Induced Arthritis in Mice Yamashita, Maya Matsumoto, Kurumi Endo, Tsutomu Ukibe, Ken Hosoya, Tomohiro Matsubara, Yumi Nakagawa, Hisako Sakai, Fumihiko Miyazaki, Tadaaki Front Microbiol Microbiology We recently reported that the intraperitoneal inoculation of Lactobacillus helveticus SBT2171 inhibited the development of collagen-induced arthritis (CIA), a murine model of rheumatoid arthritis (RA). In the present study, we evaluated the effect of the oral administration of L. helveticus SBT2171 on CIA development and on the regulation of antigen-specific antibody production and inflammatory immune cells, which have been implicated in the development of RA. Both oral administration and intraperitoneal inoculation of L. helveticus SBT2171 reduced joint swelling, body weight loss, and the serum level of bovine type II collagen (CII)-specific antibodies in the CIA mouse model. The intraperitoneal inoculation also decreased the arthritis incidence, joint damage, and serum level of interleukin (IL)-6. In addition, the numbers of total immune cells, total B cells, germinal center B cells, and CD4(+) T cells in the draining lymph nodes were decreased following intraperitoneal inoculation of L. helveticus SBT2171. These findings demonstrate the ability of L. helveticus SBT2171 to downregulate the abundance of immune cells and the subsequent production of CII-specific antibodies and IL-6, thereby suppressing the CIA symptoms, indicating its potential for use in the prevention of RA. Frontiers Media S.A. 2017-06-21 /pmc/articles/PMC5478730/ /pubmed/28680422 http://dx.doi.org/10.3389/fmicb.2017.01159 Text en Copyright © 2017 Yamashita, Matsumoto, Endo, Ukibe, Hosoya, Matsubara, Nakagawa, Sakai and Miyazaki. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Yamashita, Maya
Matsumoto, Kurumi
Endo, Tsutomu
Ukibe, Ken
Hosoya, Tomohiro
Matsubara, Yumi
Nakagawa, Hisako
Sakai, Fumihiko
Miyazaki, Tadaaki
Preventive Effect of Lactobacillus helveticus SBT2171 on Collagen-Induced Arthritis in Mice
title Preventive Effect of Lactobacillus helveticus SBT2171 on Collagen-Induced Arthritis in Mice
title_full Preventive Effect of Lactobacillus helveticus SBT2171 on Collagen-Induced Arthritis in Mice
title_fullStr Preventive Effect of Lactobacillus helveticus SBT2171 on Collagen-Induced Arthritis in Mice
title_full_unstemmed Preventive Effect of Lactobacillus helveticus SBT2171 on Collagen-Induced Arthritis in Mice
title_short Preventive Effect of Lactobacillus helveticus SBT2171 on Collagen-Induced Arthritis in Mice
title_sort preventive effect of lactobacillus helveticus sbt2171 on collagen-induced arthritis in mice
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5478730/
https://www.ncbi.nlm.nih.gov/pubmed/28680422
http://dx.doi.org/10.3389/fmicb.2017.01159
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