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Changes in serum levels of lipopolysaccharides and CD26 in patients with Crohn's disease

BACKGROUND/AIMS: Lipopolysaccharide (LPS) is a molecule formed by lipids and polysaccharides and is the major cell wall component of gram-negative bacteria. High LPS levels are known to block CD26 expression by activating Toll-like receptor 4. The aim of this study was to correlate the serum levels...

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Autores principales: Magro, Daniéla Oliveira, Kotze, Paulo Gustavo, Martinez, Carlos Augusto Real, Camargo, Michel Gardere, Guadagnini, Dioze, Calixto, Antonio Ramos, Vasques, Ana Carolina Junqueira, Ayrizono, Maria de Lourdes Setsuko, Geloneze, Bruno, Pareja, José Carlos, Saad, Mario José, Coy, Claudio Saddy Rodrigues
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Association for the Study of Intestinal Diseases 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5478760/
https://www.ncbi.nlm.nih.gov/pubmed/28670232
http://dx.doi.org/10.5217/ir.2017.15.3.352
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author Magro, Daniéla Oliveira
Kotze, Paulo Gustavo
Martinez, Carlos Augusto Real
Camargo, Michel Gardere
Guadagnini, Dioze
Calixto, Antonio Ramos
Vasques, Ana Carolina Junqueira
Ayrizono, Maria de Lourdes Setsuko
Geloneze, Bruno
Pareja, José Carlos
Saad, Mario José
Coy, Claudio Saddy Rodrigues
author_facet Magro, Daniéla Oliveira
Kotze, Paulo Gustavo
Martinez, Carlos Augusto Real
Camargo, Michel Gardere
Guadagnini, Dioze
Calixto, Antonio Ramos
Vasques, Ana Carolina Junqueira
Ayrizono, Maria de Lourdes Setsuko
Geloneze, Bruno
Pareja, José Carlos
Saad, Mario José
Coy, Claudio Saddy Rodrigues
author_sort Magro, Daniéla Oliveira
collection PubMed
description BACKGROUND/AIMS: Lipopolysaccharide (LPS) is a molecule formed by lipids and polysaccharides and is the major cell wall component of gram-negative bacteria. High LPS levels are known to block CD26 expression by activating Toll-like receptor 4. The aim of this study was to correlate the serum levels of LPS and CD26 in Crohn's disease (CD) patients with serum levels of C-reactive protein (CRP), interleukins, CD activity index, and tumor necrosis factor-α (TNF-α). METHODS: Serum samples were collected from 27 individuals (10 with active CD, 10 with inactive CD, and 7 controls) and the levels of LPS, CD26, TNF-α, interleukin-1β (IL-1β), IL-6, IL-17, and CRP were determined by enzyme-linked immunosorbent assay. The levels of LPS and CD26 were then tested for correlation with TNF-α, IL-1β, IL-6, IL-17, and CRP. RESULTS: Serum levels of LPS were significantly elevated in the active CD group (P=0.003). Levels of IL-1β (P=0.002), IL-6 (P=0.003), and IL-17 (P<0.001) were lower in the CD groups. Serum TNF-α levels were increased in the active CD group. The CRP levels were elevated in the CD groups when compared to controls (P<0.001). The CD26 levels were lower in the CD groups than in the control group (P<0.001). Among the variables analyzed, there was a correlation between LPS and CRP (r=−0.53, P=0.016) in the CD groups. CONCLUSIONS: Individuals with CD exhibited higher serum levels of LPS varying from a 2- to 6-fold increase depending on disease activity, when compared with healthy controls. CD26 levels were lower in the CD groups. Both LPS and CD26 correlated with disease severity and serve as potential CD biomarkers.
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spelling pubmed-54787602017-07-01 Changes in serum levels of lipopolysaccharides and CD26 in patients with Crohn's disease Magro, Daniéla Oliveira Kotze, Paulo Gustavo Martinez, Carlos Augusto Real Camargo, Michel Gardere Guadagnini, Dioze Calixto, Antonio Ramos Vasques, Ana Carolina Junqueira Ayrizono, Maria de Lourdes Setsuko Geloneze, Bruno Pareja, José Carlos Saad, Mario José Coy, Claudio Saddy Rodrigues Intest Res Original Article BACKGROUND/AIMS: Lipopolysaccharide (LPS) is a molecule formed by lipids and polysaccharides and is the major cell wall component of gram-negative bacteria. High LPS levels are known to block CD26 expression by activating Toll-like receptor 4. The aim of this study was to correlate the serum levels of LPS and CD26 in Crohn's disease (CD) patients with serum levels of C-reactive protein (CRP), interleukins, CD activity index, and tumor necrosis factor-α (TNF-α). METHODS: Serum samples were collected from 27 individuals (10 with active CD, 10 with inactive CD, and 7 controls) and the levels of LPS, CD26, TNF-α, interleukin-1β (IL-1β), IL-6, IL-17, and CRP were determined by enzyme-linked immunosorbent assay. The levels of LPS and CD26 were then tested for correlation with TNF-α, IL-1β, IL-6, IL-17, and CRP. RESULTS: Serum levels of LPS were significantly elevated in the active CD group (P=0.003). Levels of IL-1β (P=0.002), IL-6 (P=0.003), and IL-17 (P<0.001) were lower in the CD groups. Serum TNF-α levels were increased in the active CD group. The CRP levels were elevated in the CD groups when compared to controls (P<0.001). The CD26 levels were lower in the CD groups than in the control group (P<0.001). Among the variables analyzed, there was a correlation between LPS and CRP (r=−0.53, P=0.016) in the CD groups. CONCLUSIONS: Individuals with CD exhibited higher serum levels of LPS varying from a 2- to 6-fold increase depending on disease activity, when compared with healthy controls. CD26 levels were lower in the CD groups. Both LPS and CD26 correlated with disease severity and serve as potential CD biomarkers. Korean Association for the Study of Intestinal Diseases 2017-07 2017-06-12 /pmc/articles/PMC5478760/ /pubmed/28670232 http://dx.doi.org/10.5217/ir.2017.15.3.352 Text en © Copyright 2017. Korean Association for the Study of Intestinal Diseases. http://creativecommons.org/licenses/by-nc/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Magro, Daniéla Oliveira
Kotze, Paulo Gustavo
Martinez, Carlos Augusto Real
Camargo, Michel Gardere
Guadagnini, Dioze
Calixto, Antonio Ramos
Vasques, Ana Carolina Junqueira
Ayrizono, Maria de Lourdes Setsuko
Geloneze, Bruno
Pareja, José Carlos
Saad, Mario José
Coy, Claudio Saddy Rodrigues
Changes in serum levels of lipopolysaccharides and CD26 in patients with Crohn's disease
title Changes in serum levels of lipopolysaccharides and CD26 in patients with Crohn's disease
title_full Changes in serum levels of lipopolysaccharides and CD26 in patients with Crohn's disease
title_fullStr Changes in serum levels of lipopolysaccharides and CD26 in patients with Crohn's disease
title_full_unstemmed Changes in serum levels of lipopolysaccharides and CD26 in patients with Crohn's disease
title_short Changes in serum levels of lipopolysaccharides and CD26 in patients with Crohn's disease
title_sort changes in serum levels of lipopolysaccharides and cd26 in patients with crohn's disease
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5478760/
https://www.ncbi.nlm.nih.gov/pubmed/28670232
http://dx.doi.org/10.5217/ir.2017.15.3.352
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