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D8/17 Monoclonal Antibody: An Unclear Neuropsychiatric Marker

Objective: It has been hypothesized that monoclonal antibody D8/17 identifies a B lymphocyte antigen with expanded expression in patients with rheumatic fever, childhood onset obsessive-compulsive disorder (OCD), Tourette syndrome (TS) or prepubertal anorexia nervosa (AN). Our purpose was to replica...

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Autores principales: Morer, Astrid, Viñas, Odette, Lázaro, Luisa, Bosch, Jordi, Toro, Josep, Castro, Josefina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: IOS Press 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5478839/
https://www.ncbi.nlm.nih.gov/pubmed/16082074
http://dx.doi.org/10.1155/2005/795343
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author Morer, Astrid
Viñas, Odette
Lázaro, Luisa
Bosch, Jordi
Toro, Josep
Castro, Josefina
author_facet Morer, Astrid
Viñas, Odette
Lázaro, Luisa
Bosch, Jordi
Toro, Josep
Castro, Josefina
author_sort Morer, Astrid
collection PubMed
description Objective: It has been hypothesized that monoclonal antibody D8/17 identifies a B lymphocyte antigen with expanded expression in patients with rheumatic fever, childhood onset obsessive-compulsive disorder (OCD), Tourette syndrome (TS) or prepubertal anorexia nervosa (AN). Our purpose was to replicate these studies in a Spanish population and to determine whether D8/17 identifies a subgroup of these patients, focusing especially on OCD subjects. Method: D8/17 expression was assessed with double immunofluorescence and flow cytometry using monoclonal immunoglobulin M (IgM) in three groups of patients with diagnoses of OCD (n = 17), TS (n = 5) and prepubertal AN (n = 5), recruited during 2001. Results: In the sample studied the average percentage of B cells expressing D8/17 was 4.8%. The D8/17 positive proportion of B lymphocytes was above 11% in only two out of 17 OCD patients (7.4% of total sample) and in none of the TS or prepubertal AN patients. No statistically significant differences were found in mean percentages of D8/17 between the three groups. Conclusions: In the sample studied the expression of D8/17 in B cells was very low and the great majority of patients were negative for the D8/17 marker. The molecular characterization of D8/17 would be a major step forward in clarifying its implication for these diseases.
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spelling pubmed-54788392017-06-28 D8/17 Monoclonal Antibody: An Unclear Neuropsychiatric Marker Morer, Astrid Viñas, Odette Lázaro, Luisa Bosch, Jordi Toro, Josep Castro, Josefina Behav Neurol Other Objective: It has been hypothesized that monoclonal antibody D8/17 identifies a B lymphocyte antigen with expanded expression in patients with rheumatic fever, childhood onset obsessive-compulsive disorder (OCD), Tourette syndrome (TS) or prepubertal anorexia nervosa (AN). Our purpose was to replicate these studies in a Spanish population and to determine whether D8/17 identifies a subgroup of these patients, focusing especially on OCD subjects. Method: D8/17 expression was assessed with double immunofluorescence and flow cytometry using monoclonal immunoglobulin M (IgM) in three groups of patients with diagnoses of OCD (n = 17), TS (n = 5) and prepubertal AN (n = 5), recruited during 2001. Results: In the sample studied the average percentage of B cells expressing D8/17 was 4.8%. The D8/17 positive proportion of B lymphocytes was above 11% in only two out of 17 OCD patients (7.4% of total sample) and in none of the TS or prepubertal AN patients. No statistically significant differences were found in mean percentages of D8/17 between the three groups. Conclusions: In the sample studied the expression of D8/17 in B cells was very low and the great majority of patients were negative for the D8/17 marker. The molecular characterization of D8/17 would be a major step forward in clarifying its implication for these diseases. IOS Press 2005 2005-08-04 /pmc/articles/PMC5478839/ /pubmed/16082074 http://dx.doi.org/10.1155/2005/795343 Text en Copyright © 2005 Hindawi Publishing Corporation and the authors. http://creativecommons.org/licenses/by/3.0 This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Other
Morer, Astrid
Viñas, Odette
Lázaro, Luisa
Bosch, Jordi
Toro, Josep
Castro, Josefina
D8/17 Monoclonal Antibody: An Unclear Neuropsychiatric Marker
title D8/17 Monoclonal Antibody: An Unclear Neuropsychiatric Marker
title_full D8/17 Monoclonal Antibody: An Unclear Neuropsychiatric Marker
title_fullStr D8/17 Monoclonal Antibody: An Unclear Neuropsychiatric Marker
title_full_unstemmed D8/17 Monoclonal Antibody: An Unclear Neuropsychiatric Marker
title_short D8/17 Monoclonal Antibody: An Unclear Neuropsychiatric Marker
title_sort d8/17 monoclonal antibody: an unclear neuropsychiatric marker
topic Other
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5478839/
https://www.ncbi.nlm.nih.gov/pubmed/16082074
http://dx.doi.org/10.1155/2005/795343
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