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D8/17 Monoclonal Antibody: An Unclear Neuropsychiatric Marker
Objective: It has been hypothesized that monoclonal antibody D8/17 identifies a B lymphocyte antigen with expanded expression in patients with rheumatic fever, childhood onset obsessive-compulsive disorder (OCD), Tourette syndrome (TS) or prepubertal anorexia nervosa (AN). Our purpose was to replica...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
IOS Press
2005
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5478839/ https://www.ncbi.nlm.nih.gov/pubmed/16082074 http://dx.doi.org/10.1155/2005/795343 |
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author | Morer, Astrid Viñas, Odette Lázaro, Luisa Bosch, Jordi Toro, Josep Castro, Josefina |
author_facet | Morer, Astrid Viñas, Odette Lázaro, Luisa Bosch, Jordi Toro, Josep Castro, Josefina |
author_sort | Morer, Astrid |
collection | PubMed |
description | Objective: It has been hypothesized that monoclonal antibody D8/17 identifies a B lymphocyte antigen with expanded expression in patients with rheumatic fever, childhood onset obsessive-compulsive disorder (OCD), Tourette syndrome (TS) or prepubertal anorexia nervosa (AN). Our purpose was to replicate these studies in a Spanish population and to determine whether D8/17 identifies a subgroup of these patients, focusing especially on OCD subjects. Method: D8/17 expression was assessed with double immunofluorescence and flow cytometry using monoclonal immunoglobulin M (IgM) in three groups of patients with diagnoses of OCD (n = 17), TS (n = 5) and prepubertal AN (n = 5), recruited during 2001. Results: In the sample studied the average percentage of B cells expressing D8/17 was 4.8%. The D8/17 positive proportion of B lymphocytes was above 11% in only two out of 17 OCD patients (7.4% of total sample) and in none of the TS or prepubertal AN patients. No statistically significant differences were found in mean percentages of D8/17 between the three groups. Conclusions: In the sample studied the expression of D8/17 in B cells was very low and the great majority of patients were negative for the D8/17 marker. The molecular characterization of D8/17 would be a major step forward in clarifying its implication for these diseases. |
format | Online Article Text |
id | pubmed-5478839 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2005 |
publisher | IOS Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-54788392017-06-28 D8/17 Monoclonal Antibody: An Unclear Neuropsychiatric Marker Morer, Astrid Viñas, Odette Lázaro, Luisa Bosch, Jordi Toro, Josep Castro, Josefina Behav Neurol Other Objective: It has been hypothesized that monoclonal antibody D8/17 identifies a B lymphocyte antigen with expanded expression in patients with rheumatic fever, childhood onset obsessive-compulsive disorder (OCD), Tourette syndrome (TS) or prepubertal anorexia nervosa (AN). Our purpose was to replicate these studies in a Spanish population and to determine whether D8/17 identifies a subgroup of these patients, focusing especially on OCD subjects. Method: D8/17 expression was assessed with double immunofluorescence and flow cytometry using monoclonal immunoglobulin M (IgM) in three groups of patients with diagnoses of OCD (n = 17), TS (n = 5) and prepubertal AN (n = 5), recruited during 2001. Results: In the sample studied the average percentage of B cells expressing D8/17 was 4.8%. The D8/17 positive proportion of B lymphocytes was above 11% in only two out of 17 OCD patients (7.4% of total sample) and in none of the TS or prepubertal AN patients. No statistically significant differences were found in mean percentages of D8/17 between the three groups. Conclusions: In the sample studied the expression of D8/17 in B cells was very low and the great majority of patients were negative for the D8/17 marker. The molecular characterization of D8/17 would be a major step forward in clarifying its implication for these diseases. IOS Press 2005 2005-08-04 /pmc/articles/PMC5478839/ /pubmed/16082074 http://dx.doi.org/10.1155/2005/795343 Text en Copyright © 2005 Hindawi Publishing Corporation and the authors. http://creativecommons.org/licenses/by/3.0 This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Other Morer, Astrid Viñas, Odette Lázaro, Luisa Bosch, Jordi Toro, Josep Castro, Josefina D8/17 Monoclonal Antibody: An Unclear Neuropsychiatric Marker |
title | D8/17 Monoclonal Antibody: An Unclear Neuropsychiatric Marker |
title_full | D8/17 Monoclonal Antibody: An Unclear Neuropsychiatric Marker |
title_fullStr | D8/17 Monoclonal Antibody: An Unclear Neuropsychiatric Marker |
title_full_unstemmed | D8/17 Monoclonal Antibody: An Unclear Neuropsychiatric Marker |
title_short | D8/17 Monoclonal Antibody: An Unclear Neuropsychiatric Marker |
title_sort | d8/17 monoclonal antibody: an unclear neuropsychiatric marker |
topic | Other |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5478839/ https://www.ncbi.nlm.nih.gov/pubmed/16082074 http://dx.doi.org/10.1155/2005/795343 |
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