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miR-330-5p suppresses glioblastoma cell proliferation and invasiveness through targeting ITGA5
The present study intended to investigate the biological effects of miR-330-5p on glioblastoma (GBM) cell proliferation and invasiveness by targeting integrin α5 (ITGA5). The expressions of miR-330-5p and ITGA5 mRNA in GBM cell lines (U87, U251, and U373) and normal brain glial cell line (HEB) were...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Portland Press Ltd.
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5479020/ https://www.ncbi.nlm.nih.gov/pubmed/28336765 http://dx.doi.org/10.1042/BSR20170019 |
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author | Feng, Linsen Ma, Jianhua Ji, Haiming Liu, Yichun Hu, Weixing |
author_facet | Feng, Linsen Ma, Jianhua Ji, Haiming Liu, Yichun Hu, Weixing |
author_sort | Feng, Linsen |
collection | PubMed |
description | The present study intended to investigate the biological effects of miR-330-5p on glioblastoma (GBM) cell proliferation and invasiveness by targeting integrin α5 (ITGA5). The expressions of miR-330-5p and ITGA5 mRNA in GBM cell lines (U87, U251, and U373) and normal brain glial cell line (HEB) were detected using RT-qPCR. Protein expression of ITGA5 was examined using Western blot. The present study used MTT assay, colony formation assay, Transwell assay, wound healing assay, and flow cytometry analysis in order to determine the biological functions of GBM cells (including cell proliferation, invasion, migration, apoptosis, and cell cycle). The present study applied dual-luciferase reporter gene assay to identify the target relationship between miR-330-5p and ITGA5. miR-330-5p was low-expressed in GBM cell lines while ITGA5 was high-expressed compared with HEB. miR-330-5p could directly target ITGA5 as well as suppress its expression in GBM cells. Up-regulation of miR-330-5p and down-regulation of ITGA5 both have an inhibitory effect on cell proliferation, invasion, and migration. Meanwhile, they could also promote GBM cell apoptosis. miR-330-5p could suppress proliferation and invasion of GBM cells through targeting ITGA5. |
format | Online Article Text |
id | pubmed-5479020 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Portland Press Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-54790202017-06-28 miR-330-5p suppresses glioblastoma cell proliferation and invasiveness through targeting ITGA5 Feng, Linsen Ma, Jianhua Ji, Haiming Liu, Yichun Hu, Weixing Biosci Rep Research Articles The present study intended to investigate the biological effects of miR-330-5p on glioblastoma (GBM) cell proliferation and invasiveness by targeting integrin α5 (ITGA5). The expressions of miR-330-5p and ITGA5 mRNA in GBM cell lines (U87, U251, and U373) and normal brain glial cell line (HEB) were detected using RT-qPCR. Protein expression of ITGA5 was examined using Western blot. The present study used MTT assay, colony formation assay, Transwell assay, wound healing assay, and flow cytometry analysis in order to determine the biological functions of GBM cells (including cell proliferation, invasion, migration, apoptosis, and cell cycle). The present study applied dual-luciferase reporter gene assay to identify the target relationship between miR-330-5p and ITGA5. miR-330-5p was low-expressed in GBM cell lines while ITGA5 was high-expressed compared with HEB. miR-330-5p could directly target ITGA5 as well as suppress its expression in GBM cells. Up-regulation of miR-330-5p and down-regulation of ITGA5 both have an inhibitory effect on cell proliferation, invasion, and migration. Meanwhile, they could also promote GBM cell apoptosis. miR-330-5p could suppress proliferation and invasion of GBM cells through targeting ITGA5. Portland Press Ltd. 2017-06-21 /pmc/articles/PMC5479020/ /pubmed/28336765 http://dx.doi.org/10.1042/BSR20170019 Text en © 2017 The Author(s). http://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (http://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Articles Feng, Linsen Ma, Jianhua Ji, Haiming Liu, Yichun Hu, Weixing miR-330-5p suppresses glioblastoma cell proliferation and invasiveness through targeting ITGA5 |
title | miR-330-5p suppresses glioblastoma cell proliferation and invasiveness through targeting ITGA5 |
title_full | miR-330-5p suppresses glioblastoma cell proliferation and invasiveness through targeting ITGA5 |
title_fullStr | miR-330-5p suppresses glioblastoma cell proliferation and invasiveness through targeting ITGA5 |
title_full_unstemmed | miR-330-5p suppresses glioblastoma cell proliferation and invasiveness through targeting ITGA5 |
title_short | miR-330-5p suppresses glioblastoma cell proliferation and invasiveness through targeting ITGA5 |
title_sort | mir-330-5p suppresses glioblastoma cell proliferation and invasiveness through targeting itga5 |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5479020/ https://www.ncbi.nlm.nih.gov/pubmed/28336765 http://dx.doi.org/10.1042/BSR20170019 |
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