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Bioactivities of ethanol extract from the Antarctic freshwater microalga, Chloromonas sp.

Cancer is the principal cause of human death and occurs through highly complex processes that involve the multiple coordinated mechanisms of tumorigenesis. A number of studies have indicated that the microalgae extracts showed anticancer activity in a variety of human cancer cells and can provide a...

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Autores principales: Suh, Sung-Suk, Yang, Eun Jin, Lee, Sung Gu, Youn, Ui Joung, Han, Se Jong, Kim, Il-Chan, Kim, Sanghee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5479125/
https://www.ncbi.nlm.nih.gov/pubmed/28638272
http://dx.doi.org/10.7150/ijms.18702
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author Suh, Sung-Suk
Yang, Eun Jin
Lee, Sung Gu
Youn, Ui Joung
Han, Se Jong
Kim, Il-Chan
Kim, Sanghee
author_facet Suh, Sung-Suk
Yang, Eun Jin
Lee, Sung Gu
Youn, Ui Joung
Han, Se Jong
Kim, Il-Chan
Kim, Sanghee
author_sort Suh, Sung-Suk
collection PubMed
description Cancer is the principal cause of human death and occurs through highly complex processes that involve the multiple coordinated mechanisms of tumorigenesis. A number of studies have indicated that the microalgae extracts showed anticancer activity in a variety of human cancer cells and can provide a new insight in the development of novel anti-cancer therapy. Here, in order to investigate molecular mechanisms of anticancer activity in the Antarctic freshwater microalga, Chloromonas sp., we prepared ethanol extract of Chloromonas sp. (ETCH) and performed several in vitro assays using human normal keratinocyte (HaCaT) and different types of cancer cells including cervical, melanoma, and breast cancer cells (HeLa, A375 and Hs578T, respectively). We revealed that ETCH had the antioxidant capacity, and caused significant cell growth inhibition and apoptosis of cancer cells in a dose-dependent manner, whereas it showed no anti-proliferation to normal cells. In addition, ETCH had a significant inhibitory effect on cell invasion without the cytotoxic effect. Furthermore, ETCH-induced apoptosis was mediated by increase in pro-apoptotic proteins including cleaved caspase-3 and p53, and by decrease in anti-apoptotic protein, Bcl-2 in ETCH-treated cancer cells. Taken together, this work firstly explored the antioxidant and anticancer activities of an Antarctic freshwater microalga, and ETCH could be a potential therapeutic candidate in the treatment of human cancer.
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spelling pubmed-54791252017-06-21 Bioactivities of ethanol extract from the Antarctic freshwater microalga, Chloromonas sp. Suh, Sung-Suk Yang, Eun Jin Lee, Sung Gu Youn, Ui Joung Han, Se Jong Kim, Il-Chan Kim, Sanghee Int J Med Sci Research Paper Cancer is the principal cause of human death and occurs through highly complex processes that involve the multiple coordinated mechanisms of tumorigenesis. A number of studies have indicated that the microalgae extracts showed anticancer activity in a variety of human cancer cells and can provide a new insight in the development of novel anti-cancer therapy. Here, in order to investigate molecular mechanisms of anticancer activity in the Antarctic freshwater microalga, Chloromonas sp., we prepared ethanol extract of Chloromonas sp. (ETCH) and performed several in vitro assays using human normal keratinocyte (HaCaT) and different types of cancer cells including cervical, melanoma, and breast cancer cells (HeLa, A375 and Hs578T, respectively). We revealed that ETCH had the antioxidant capacity, and caused significant cell growth inhibition and apoptosis of cancer cells in a dose-dependent manner, whereas it showed no anti-proliferation to normal cells. In addition, ETCH had a significant inhibitory effect on cell invasion without the cytotoxic effect. Furthermore, ETCH-induced apoptosis was mediated by increase in pro-apoptotic proteins including cleaved caspase-3 and p53, and by decrease in anti-apoptotic protein, Bcl-2 in ETCH-treated cancer cells. Taken together, this work firstly explored the antioxidant and anticancer activities of an Antarctic freshwater microalga, and ETCH could be a potential therapeutic candidate in the treatment of human cancer. Ivyspring International Publisher 2017-04-28 /pmc/articles/PMC5479125/ /pubmed/28638272 http://dx.doi.org/10.7150/ijms.18702 Text en © Ivyspring International Publisher This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/). See http://ivyspring.com/terms for full terms and conditions.
spellingShingle Research Paper
Suh, Sung-Suk
Yang, Eun Jin
Lee, Sung Gu
Youn, Ui Joung
Han, Se Jong
Kim, Il-Chan
Kim, Sanghee
Bioactivities of ethanol extract from the Antarctic freshwater microalga, Chloromonas sp.
title Bioactivities of ethanol extract from the Antarctic freshwater microalga, Chloromonas sp.
title_full Bioactivities of ethanol extract from the Antarctic freshwater microalga, Chloromonas sp.
title_fullStr Bioactivities of ethanol extract from the Antarctic freshwater microalga, Chloromonas sp.
title_full_unstemmed Bioactivities of ethanol extract from the Antarctic freshwater microalga, Chloromonas sp.
title_short Bioactivities of ethanol extract from the Antarctic freshwater microalga, Chloromonas sp.
title_sort bioactivities of ethanol extract from the antarctic freshwater microalga, chloromonas sp.
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5479125/
https://www.ncbi.nlm.nih.gov/pubmed/28638272
http://dx.doi.org/10.7150/ijms.18702
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