Cargando…

The Application of Dehydroepiandrosterone on Improving Mitochondrial Function and Reducing Apoptosis of Cumulus Cells in Poor Ovarian Responders

Poor ovarian responders (PORs) pose a great challenge for in vitro fertilization (IVF). Previous studies have suggested that dehydroepiandrosterone (DHEA) may improve IVF outcomes in PORs. The current study attempted to investigate the clinical benefits of DHEA in PORs and the possible mechanisms of...

Descripción completa

Detalles Bibliográficos
Autores principales: Lin, Li-Te, Wang, Peng-Hui, Wen, Zhi-Hong, Li, Chia-Jung, Chen, San-Nung, Tsai, Eing-Mei, Cheng, Jiin-Tsuey, Tsui, Kuan-Hao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5479128/
https://www.ncbi.nlm.nih.gov/pubmed/28638275
http://dx.doi.org/10.7150/ijms.18706
_version_ 1783245078383296512
author Lin, Li-Te
Wang, Peng-Hui
Wen, Zhi-Hong
Li, Chia-Jung
Chen, San-Nung
Tsai, Eing-Mei
Cheng, Jiin-Tsuey
Tsui, Kuan-Hao
author_facet Lin, Li-Te
Wang, Peng-Hui
Wen, Zhi-Hong
Li, Chia-Jung
Chen, San-Nung
Tsai, Eing-Mei
Cheng, Jiin-Tsuey
Tsui, Kuan-Hao
author_sort Lin, Li-Te
collection PubMed
description Poor ovarian responders (PORs) pose a great challenge for in vitro fertilization (IVF). Previous studies have suggested that dehydroepiandrosterone (DHEA) may improve IVF outcomes in PORs. The current study attempted to investigate the clinical benefits of DHEA in PORs and the possible mechanisms of DHEA on cumulus cells (CCs). This was a prospective study performed at one tertiary center from January 2015 to March 2016. A total of 131 women who underwent IVF treatment participated, including 59 normal ovarian responders (NORs) and 72 PORs. PORs were assigned to receive DHEA supplementation or not before the IVF cycle. For all patients, CCs were obtained after oocyte retrieval. In the CCs, mRNA expression of apoptosis-related genes and mitochondrial transcription factor A (TFAM) gene, terminal deoxynucleotidyl transferase dUTP nick end labeling assay, mitochondrial dehydrogenase activity and mitochondrial mass were measured. The results indicated that PORs with DHEA supplementation produces a great number of top-quality embryos at day 3 and increased the number of transferred embryos and fertilization rate compared with those without DHEA supplementation. Additionally, supplementation with DHEA in PORs decreased DNA damage and apoptosis in CCs while enhancing the mitochondrial mass, mitochondrial dehydrogenase activity and TFAM expression in CCs. In conclusion, our results showed that the benefits of DHEA supplementation on IVF outcomes in PORs were significant, and the effects may be partially mediated by improving mitochondrial function and reducing apoptosis in CCs.
format Online
Article
Text
id pubmed-5479128
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Ivyspring International Publisher
record_format MEDLINE/PubMed
spelling pubmed-54791282017-06-21 The Application of Dehydroepiandrosterone on Improving Mitochondrial Function and Reducing Apoptosis of Cumulus Cells in Poor Ovarian Responders Lin, Li-Te Wang, Peng-Hui Wen, Zhi-Hong Li, Chia-Jung Chen, San-Nung Tsai, Eing-Mei Cheng, Jiin-Tsuey Tsui, Kuan-Hao Int J Med Sci Research Paper Poor ovarian responders (PORs) pose a great challenge for in vitro fertilization (IVF). Previous studies have suggested that dehydroepiandrosterone (DHEA) may improve IVF outcomes in PORs. The current study attempted to investigate the clinical benefits of DHEA in PORs and the possible mechanisms of DHEA on cumulus cells (CCs). This was a prospective study performed at one tertiary center from January 2015 to March 2016. A total of 131 women who underwent IVF treatment participated, including 59 normal ovarian responders (NORs) and 72 PORs. PORs were assigned to receive DHEA supplementation or not before the IVF cycle. For all patients, CCs were obtained after oocyte retrieval. In the CCs, mRNA expression of apoptosis-related genes and mitochondrial transcription factor A (TFAM) gene, terminal deoxynucleotidyl transferase dUTP nick end labeling assay, mitochondrial dehydrogenase activity and mitochondrial mass were measured. The results indicated that PORs with DHEA supplementation produces a great number of top-quality embryos at day 3 and increased the number of transferred embryos and fertilization rate compared with those without DHEA supplementation. Additionally, supplementation with DHEA in PORs decreased DNA damage and apoptosis in CCs while enhancing the mitochondrial mass, mitochondrial dehydrogenase activity and TFAM expression in CCs. In conclusion, our results showed that the benefits of DHEA supplementation on IVF outcomes in PORs were significant, and the effects may be partially mediated by improving mitochondrial function and reducing apoptosis in CCs. Ivyspring International Publisher 2017-05-13 /pmc/articles/PMC5479128/ /pubmed/28638275 http://dx.doi.org/10.7150/ijms.18706 Text en © Ivyspring International Publisher This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/). See http://ivyspring.com/terms for full terms and conditions.
spellingShingle Research Paper
Lin, Li-Te
Wang, Peng-Hui
Wen, Zhi-Hong
Li, Chia-Jung
Chen, San-Nung
Tsai, Eing-Mei
Cheng, Jiin-Tsuey
Tsui, Kuan-Hao
The Application of Dehydroepiandrosterone on Improving Mitochondrial Function and Reducing Apoptosis of Cumulus Cells in Poor Ovarian Responders
title The Application of Dehydroepiandrosterone on Improving Mitochondrial Function and Reducing Apoptosis of Cumulus Cells in Poor Ovarian Responders
title_full The Application of Dehydroepiandrosterone on Improving Mitochondrial Function and Reducing Apoptosis of Cumulus Cells in Poor Ovarian Responders
title_fullStr The Application of Dehydroepiandrosterone on Improving Mitochondrial Function and Reducing Apoptosis of Cumulus Cells in Poor Ovarian Responders
title_full_unstemmed The Application of Dehydroepiandrosterone on Improving Mitochondrial Function and Reducing Apoptosis of Cumulus Cells in Poor Ovarian Responders
title_short The Application of Dehydroepiandrosterone on Improving Mitochondrial Function and Reducing Apoptosis of Cumulus Cells in Poor Ovarian Responders
title_sort application of dehydroepiandrosterone on improving mitochondrial function and reducing apoptosis of cumulus cells in poor ovarian responders
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5479128/
https://www.ncbi.nlm.nih.gov/pubmed/28638275
http://dx.doi.org/10.7150/ijms.18706
work_keys_str_mv AT linlite theapplicationofdehydroepiandrosteroneonimprovingmitochondrialfunctionandreducingapoptosisofcumuluscellsinpoorovarianresponders
AT wangpenghui theapplicationofdehydroepiandrosteroneonimprovingmitochondrialfunctionandreducingapoptosisofcumuluscellsinpoorovarianresponders
AT wenzhihong theapplicationofdehydroepiandrosteroneonimprovingmitochondrialfunctionandreducingapoptosisofcumuluscellsinpoorovarianresponders
AT lichiajung theapplicationofdehydroepiandrosteroneonimprovingmitochondrialfunctionandreducingapoptosisofcumuluscellsinpoorovarianresponders
AT chensannung theapplicationofdehydroepiandrosteroneonimprovingmitochondrialfunctionandreducingapoptosisofcumuluscellsinpoorovarianresponders
AT tsaieingmei theapplicationofdehydroepiandrosteroneonimprovingmitochondrialfunctionandreducingapoptosisofcumuluscellsinpoorovarianresponders
AT chengjiintsuey theapplicationofdehydroepiandrosteroneonimprovingmitochondrialfunctionandreducingapoptosisofcumuluscellsinpoorovarianresponders
AT tsuikuanhao theapplicationofdehydroepiandrosteroneonimprovingmitochondrialfunctionandreducingapoptosisofcumuluscellsinpoorovarianresponders
AT linlite applicationofdehydroepiandrosteroneonimprovingmitochondrialfunctionandreducingapoptosisofcumuluscellsinpoorovarianresponders
AT wangpenghui applicationofdehydroepiandrosteroneonimprovingmitochondrialfunctionandreducingapoptosisofcumuluscellsinpoorovarianresponders
AT wenzhihong applicationofdehydroepiandrosteroneonimprovingmitochondrialfunctionandreducingapoptosisofcumuluscellsinpoorovarianresponders
AT lichiajung applicationofdehydroepiandrosteroneonimprovingmitochondrialfunctionandreducingapoptosisofcumuluscellsinpoorovarianresponders
AT chensannung applicationofdehydroepiandrosteroneonimprovingmitochondrialfunctionandreducingapoptosisofcumuluscellsinpoorovarianresponders
AT tsaieingmei applicationofdehydroepiandrosteroneonimprovingmitochondrialfunctionandreducingapoptosisofcumuluscellsinpoorovarianresponders
AT chengjiintsuey applicationofdehydroepiandrosteroneonimprovingmitochondrialfunctionandreducingapoptosisofcumuluscellsinpoorovarianresponders
AT tsuikuanhao applicationofdehydroepiandrosteroneonimprovingmitochondrialfunctionandreducingapoptosisofcumuluscellsinpoorovarianresponders