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An endophytic Basidiomycete, Grammothele lineata, isolated from Corchorus olitorius, produces paclitaxel that shows cytotoxicity
Grammothele lineata, an endophyte isolated in our laboratory from jute (Corchorus olitorius acc. 2015) was found to be a substantial paclitaxel producer. Taxol and its related compounds, produced by this endophyte were extracted by growing the fungus in simple nutrient media (potato dextrose broth,...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Public Library of Science
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5479517/ https://www.ncbi.nlm.nih.gov/pubmed/28636663 http://dx.doi.org/10.1371/journal.pone.0178612 |
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author | Das, Avizit Rahman, Mohammad Imtiazur Ferdous, Ahlan Sabah Amin, Al- Rahman, Mohammad Mahbubur Nahar, Nilufar Uddin, Md. Aftab Islam, Mohammad Riazul Khan, Haseena |
author_facet | Das, Avizit Rahman, Mohammad Imtiazur Ferdous, Ahlan Sabah Amin, Al- Rahman, Mohammad Mahbubur Nahar, Nilufar Uddin, Md. Aftab Islam, Mohammad Riazul Khan, Haseena |
author_sort | Das, Avizit |
collection | PubMed |
description | Grammothele lineata, an endophyte isolated in our laboratory from jute (Corchorus olitorius acc. 2015) was found to be a substantial paclitaxel producer. Taxol and its related compounds, produced by this endophyte were extracted by growing the fungus in simple nutrient media (potato dextrose broth, PDB). Taxol was identified and characterized by different analytical techniques (TLC, HPLC, FTIR, LC-ESI-MS/MS) following its extraction by ethyl acetate. In PDB media, this fungus was found to produce 382.2 μgL(-1) of taxol which is about 7.6 x10(3) fold higher than the first reported endophytic fungi, Taxomyces andreanae. The extracted taxol exhibited cytotoxic activity in an in vitro culture of HeLa cancer cell line. The fungal extract also exhibited antifungal and antibacterial activities against different pathogenic strains. This is the first report of a jute endophytic fungus harboring the capacity to produce taxol and also the first reported taxol producing species that belongs to the Basidiomycota phylum, so far unknown to be a taxol producer. These findings suggest that the fungal endophyte, Grammothele lineata can be an excellent source of taxol and can also serve as a potential species for chemical and genetic engineering to enhance further the production of taxol. |
format | Online Article Text |
id | pubmed-5479517 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-54795172017-07-05 An endophytic Basidiomycete, Grammothele lineata, isolated from Corchorus olitorius, produces paclitaxel that shows cytotoxicity Das, Avizit Rahman, Mohammad Imtiazur Ferdous, Ahlan Sabah Amin, Al- Rahman, Mohammad Mahbubur Nahar, Nilufar Uddin, Md. Aftab Islam, Mohammad Riazul Khan, Haseena PLoS One Research Article Grammothele lineata, an endophyte isolated in our laboratory from jute (Corchorus olitorius acc. 2015) was found to be a substantial paclitaxel producer. Taxol and its related compounds, produced by this endophyte were extracted by growing the fungus in simple nutrient media (potato dextrose broth, PDB). Taxol was identified and characterized by different analytical techniques (TLC, HPLC, FTIR, LC-ESI-MS/MS) following its extraction by ethyl acetate. In PDB media, this fungus was found to produce 382.2 μgL(-1) of taxol which is about 7.6 x10(3) fold higher than the first reported endophytic fungi, Taxomyces andreanae. The extracted taxol exhibited cytotoxic activity in an in vitro culture of HeLa cancer cell line. The fungal extract also exhibited antifungal and antibacterial activities against different pathogenic strains. This is the first report of a jute endophytic fungus harboring the capacity to produce taxol and also the first reported taxol producing species that belongs to the Basidiomycota phylum, so far unknown to be a taxol producer. These findings suggest that the fungal endophyte, Grammothele lineata can be an excellent source of taxol and can also serve as a potential species for chemical and genetic engineering to enhance further the production of taxol. Public Library of Science 2017-06-21 /pmc/articles/PMC5479517/ /pubmed/28636663 http://dx.doi.org/10.1371/journal.pone.0178612 Text en © 2017 Das et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Das, Avizit Rahman, Mohammad Imtiazur Ferdous, Ahlan Sabah Amin, Al- Rahman, Mohammad Mahbubur Nahar, Nilufar Uddin, Md. Aftab Islam, Mohammad Riazul Khan, Haseena An endophytic Basidiomycete, Grammothele lineata, isolated from Corchorus olitorius, produces paclitaxel that shows cytotoxicity |
title | An endophytic Basidiomycete, Grammothele lineata, isolated from Corchorus olitorius, produces paclitaxel that shows cytotoxicity |
title_full | An endophytic Basidiomycete, Grammothele lineata, isolated from Corchorus olitorius, produces paclitaxel that shows cytotoxicity |
title_fullStr | An endophytic Basidiomycete, Grammothele lineata, isolated from Corchorus olitorius, produces paclitaxel that shows cytotoxicity |
title_full_unstemmed | An endophytic Basidiomycete, Grammothele lineata, isolated from Corchorus olitorius, produces paclitaxel that shows cytotoxicity |
title_short | An endophytic Basidiomycete, Grammothele lineata, isolated from Corchorus olitorius, produces paclitaxel that shows cytotoxicity |
title_sort | endophytic basidiomycete, grammothele lineata, isolated from corchorus olitorius, produces paclitaxel that shows cytotoxicity |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5479517/ https://www.ncbi.nlm.nih.gov/pubmed/28636663 http://dx.doi.org/10.1371/journal.pone.0178612 |
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