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Microbiota-derived butyrate suppresses group 3 innate lymphoid cells in terminal ileal Peyer’s patches
The regional specialization of intestinal immune cells is affected by the longitudinal heterogeneity of environmental factors. Although the distribution of group 3 innate lymphoid cells (ILC3s) is well characterized in the lamina propria, it is poorly defined in Peyer’s patches (PPs) along the intes...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5479798/ https://www.ncbi.nlm.nih.gov/pubmed/28638068 http://dx.doi.org/10.1038/s41598-017-02729-6 |
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author | Kim, Sae-Hae Cho, Byeol-Hee Kiyono, Hiroshi Jang, Yong-Suk |
author_facet | Kim, Sae-Hae Cho, Byeol-Hee Kiyono, Hiroshi Jang, Yong-Suk |
author_sort | Kim, Sae-Hae |
collection | PubMed |
description | The regional specialization of intestinal immune cells is affected by the longitudinal heterogeneity of environmental factors. Although the distribution of group 3 innate lymphoid cells (ILC3s) is well characterized in the lamina propria, it is poorly defined in Peyer’s patches (PPs) along the intestine. Given that PP ILC3s are closely associated with mucosal immune regulation, it is important to characterize the regulatory mechanism of ILC3s. Here, we found that terminal ileal PPs of specific pathogen-free (SPF) mice have fewer NKp46(+) ILC3s than jejunal PPs, while there was no difference in NKp46(+) ILC3 numbers between terminal ileal and jejunal PPs in antibiotics (ABX)-treated mice. We also found that butyrate levels in the terminal ileal PPs of SPF mice were higher than those in the jejunal PPs of SPF mice and terminal ileal PPs of ABX-treated mice. The reduced number of NKp46(+) ILC3s in terminal ileal PPs resulted in a decrease in Csf2 expression and, in turn, resulted in reduced regulatory T cells and enhanced antigen-specific T-cell proliferation. Thus, we suggest that NKp46(+) ILC3s are negatively regulated by microbiota-derived butyrate in terminal ileal PPs and the reduced ILC3 frequency is closely associated with antigen-specific immune induction in terminal ileal PPs. |
format | Online Article Text |
id | pubmed-5479798 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-54797982017-06-23 Microbiota-derived butyrate suppresses group 3 innate lymphoid cells in terminal ileal Peyer’s patches Kim, Sae-Hae Cho, Byeol-Hee Kiyono, Hiroshi Jang, Yong-Suk Sci Rep Article The regional specialization of intestinal immune cells is affected by the longitudinal heterogeneity of environmental factors. Although the distribution of group 3 innate lymphoid cells (ILC3s) is well characterized in the lamina propria, it is poorly defined in Peyer’s patches (PPs) along the intestine. Given that PP ILC3s are closely associated with mucosal immune regulation, it is important to characterize the regulatory mechanism of ILC3s. Here, we found that terminal ileal PPs of specific pathogen-free (SPF) mice have fewer NKp46(+) ILC3s than jejunal PPs, while there was no difference in NKp46(+) ILC3 numbers between terminal ileal and jejunal PPs in antibiotics (ABX)-treated mice. We also found that butyrate levels in the terminal ileal PPs of SPF mice were higher than those in the jejunal PPs of SPF mice and terminal ileal PPs of ABX-treated mice. The reduced number of NKp46(+) ILC3s in terminal ileal PPs resulted in a decrease in Csf2 expression and, in turn, resulted in reduced regulatory T cells and enhanced antigen-specific T-cell proliferation. Thus, we suggest that NKp46(+) ILC3s are negatively regulated by microbiota-derived butyrate in terminal ileal PPs and the reduced ILC3 frequency is closely associated with antigen-specific immune induction in terminal ileal PPs. Nature Publishing Group UK 2017-06-21 /pmc/articles/PMC5479798/ /pubmed/28638068 http://dx.doi.org/10.1038/s41598-017-02729-6 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Kim, Sae-Hae Cho, Byeol-Hee Kiyono, Hiroshi Jang, Yong-Suk Microbiota-derived butyrate suppresses group 3 innate lymphoid cells in terminal ileal Peyer’s patches |
title | Microbiota-derived butyrate suppresses group 3 innate lymphoid cells in terminal ileal Peyer’s patches |
title_full | Microbiota-derived butyrate suppresses group 3 innate lymphoid cells in terminal ileal Peyer’s patches |
title_fullStr | Microbiota-derived butyrate suppresses group 3 innate lymphoid cells in terminal ileal Peyer’s patches |
title_full_unstemmed | Microbiota-derived butyrate suppresses group 3 innate lymphoid cells in terminal ileal Peyer’s patches |
title_short | Microbiota-derived butyrate suppresses group 3 innate lymphoid cells in terminal ileal Peyer’s patches |
title_sort | microbiota-derived butyrate suppresses group 3 innate lymphoid cells in terminal ileal peyer’s patches |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5479798/ https://www.ncbi.nlm.nih.gov/pubmed/28638068 http://dx.doi.org/10.1038/s41598-017-02729-6 |
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