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Transcriptome analyses of taste organoids reveal multiple pathways involved in taste cell generation

Taste cells undergo constant turnover throughout life; however, the molecular mechanisms governing taste cell generation are not well understood. Using RNA-Seq, we systematically surveyed the transcriptome landscape of taste organoids at different stages of growth. Our data show the staged expressio...

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Autores principales: Ren, Wenwen, Aihara, Eitaro, Lei, Weiwei, Gheewala, Nishi, Uchiyama, Hironobu, Margolskee, Robert F., Iwatsuki, Ken, Jiang, Peihua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5479815/
https://www.ncbi.nlm.nih.gov/pubmed/28638111
http://dx.doi.org/10.1038/s41598-017-04099-5
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author Ren, Wenwen
Aihara, Eitaro
Lei, Weiwei
Gheewala, Nishi
Uchiyama, Hironobu
Margolskee, Robert F.
Iwatsuki, Ken
Jiang, Peihua
author_facet Ren, Wenwen
Aihara, Eitaro
Lei, Weiwei
Gheewala, Nishi
Uchiyama, Hironobu
Margolskee, Robert F.
Iwatsuki, Ken
Jiang, Peihua
author_sort Ren, Wenwen
collection PubMed
description Taste cells undergo constant turnover throughout life; however, the molecular mechanisms governing taste cell generation are not well understood. Using RNA-Seq, we systematically surveyed the transcriptome landscape of taste organoids at different stages of growth. Our data show the staged expression of a variety of genes and identify multiple signaling pathways underlying taste cell differentiation and taste stem/progenitor cell proliferation. For example, transcripts of taste receptors appear only or predominantly in late-stage organoids. Prior to that, transcription factors and other signaling elements are upregulated. RNA-Seq identified a number of well-characterized signaling pathways in taste organoid cultures, such as those involving Wnt, bone morphogenetic proteins (BMPs), Notch, and Hedgehog (Hh). By pharmacological manipulation, we demonstrate that Wnt, BMPs, Notch, and Hh signaling pathways are necessary for taste cell proliferation, differentiation and cell fate determination. The temporal expression profiles displayed by taste organoids may also lead to the identification of currently unknown transducer elements underlying sour, salt, and other taste qualities, given the staged expression of taste receptor genes and taste transduction elements in cultured organoids.
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spelling pubmed-54798152017-06-23 Transcriptome analyses of taste organoids reveal multiple pathways involved in taste cell generation Ren, Wenwen Aihara, Eitaro Lei, Weiwei Gheewala, Nishi Uchiyama, Hironobu Margolskee, Robert F. Iwatsuki, Ken Jiang, Peihua Sci Rep Article Taste cells undergo constant turnover throughout life; however, the molecular mechanisms governing taste cell generation are not well understood. Using RNA-Seq, we systematically surveyed the transcriptome landscape of taste organoids at different stages of growth. Our data show the staged expression of a variety of genes and identify multiple signaling pathways underlying taste cell differentiation and taste stem/progenitor cell proliferation. For example, transcripts of taste receptors appear only or predominantly in late-stage organoids. Prior to that, transcription factors and other signaling elements are upregulated. RNA-Seq identified a number of well-characterized signaling pathways in taste organoid cultures, such as those involving Wnt, bone morphogenetic proteins (BMPs), Notch, and Hedgehog (Hh). By pharmacological manipulation, we demonstrate that Wnt, BMPs, Notch, and Hh signaling pathways are necessary for taste cell proliferation, differentiation and cell fate determination. The temporal expression profiles displayed by taste organoids may also lead to the identification of currently unknown transducer elements underlying sour, salt, and other taste qualities, given the staged expression of taste receptor genes and taste transduction elements in cultured organoids. Nature Publishing Group UK 2017-06-21 /pmc/articles/PMC5479815/ /pubmed/28638111 http://dx.doi.org/10.1038/s41598-017-04099-5 Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Ren, Wenwen
Aihara, Eitaro
Lei, Weiwei
Gheewala, Nishi
Uchiyama, Hironobu
Margolskee, Robert F.
Iwatsuki, Ken
Jiang, Peihua
Transcriptome analyses of taste organoids reveal multiple pathways involved in taste cell generation
title Transcriptome analyses of taste organoids reveal multiple pathways involved in taste cell generation
title_full Transcriptome analyses of taste organoids reveal multiple pathways involved in taste cell generation
title_fullStr Transcriptome analyses of taste organoids reveal multiple pathways involved in taste cell generation
title_full_unstemmed Transcriptome analyses of taste organoids reveal multiple pathways involved in taste cell generation
title_short Transcriptome analyses of taste organoids reveal multiple pathways involved in taste cell generation
title_sort transcriptome analyses of taste organoids reveal multiple pathways involved in taste cell generation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5479815/
https://www.ncbi.nlm.nih.gov/pubmed/28638111
http://dx.doi.org/10.1038/s41598-017-04099-5
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