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Chloride intracellular channel 1 regulates the antineoplastic effects of metformin in gallbladder cancer cells
Metformin is the most commonly used drug for type 2 diabetes and has potential benefit in treating and preventing cancer. Previous studies indicated that membrane proteins can affect the antineoplastic effects of metformin and may be crucial in the field of cancer research. However, the antineoplast...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5480064/ https://www.ncbi.nlm.nih.gov/pubmed/28378944 http://dx.doi.org/10.1111/cas.13248 |
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author | Liu, Yongchen Wang, Zheng Li, Maolan Ye, Yuanyuan Xu, Yi Zhang, Yichi Yuan, Ruiyan Jin, Yunpeng Hao, Yajuan Jiang, Lin Hu, Yunping Chen, Shili Liu, Fatao Zhang, Yijian Wu, Wenguang Liu, Yingbin |
author_facet | Liu, Yongchen Wang, Zheng Li, Maolan Ye, Yuanyuan Xu, Yi Zhang, Yichi Yuan, Ruiyan Jin, Yunpeng Hao, Yajuan Jiang, Lin Hu, Yunping Chen, Shili Liu, Fatao Zhang, Yijian Wu, Wenguang Liu, Yingbin |
author_sort | Liu, Yongchen |
collection | PubMed |
description | Metformin is the most commonly used drug for type 2 diabetes and has potential benefit in treating and preventing cancer. Previous studies indicated that membrane proteins can affect the antineoplastic effects of metformin and may be crucial in the field of cancer research. However, the antineoplastic effects of metformin and its mechanism in gallbladder cancer (GBC) remain largely unknown. In this study, the effects of metformin on GBC cell proliferation and viability were evaluated using the Cell Counting Kit‐8 (CCK‐8) assay and an apoptosis assay. Western blotting was performed to investigate related signaling pathways. Of note, inhibition, knockdown and upregulation of the membrane protein Chloride intracellular channel 1 (CLIC1) can affect GBC resistance in the presence of metformin. Our data demonstrated that metformin apparently inhibits the proliferation and viability of GBC cells. Metformin promoted cell apoptosis and increased the number of early apoptotic cells. We found that metformin can exert growth‐suppressive effects on these cell lines via inhibition of p‐Akt activity and the Bcl‐2 family. Notably, either dysfunction or downregulation of CLIC1 can partially decrease the antineoplastic effects of metformin while upregulation of CLIC1 can increase drug sensitivity. Our findings provide experimental evidence for using metformin as an antitumor treatment for gallbladder carcinoma. |
format | Online Article Text |
id | pubmed-5480064 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-54800642017-06-23 Chloride intracellular channel 1 regulates the antineoplastic effects of metformin in gallbladder cancer cells Liu, Yongchen Wang, Zheng Li, Maolan Ye, Yuanyuan Xu, Yi Zhang, Yichi Yuan, Ruiyan Jin, Yunpeng Hao, Yajuan Jiang, Lin Hu, Yunping Chen, Shili Liu, Fatao Zhang, Yijian Wu, Wenguang Liu, Yingbin Cancer Sci Original Articles Metformin is the most commonly used drug for type 2 diabetes and has potential benefit in treating and preventing cancer. Previous studies indicated that membrane proteins can affect the antineoplastic effects of metformin and may be crucial in the field of cancer research. However, the antineoplastic effects of metformin and its mechanism in gallbladder cancer (GBC) remain largely unknown. In this study, the effects of metformin on GBC cell proliferation and viability were evaluated using the Cell Counting Kit‐8 (CCK‐8) assay and an apoptosis assay. Western blotting was performed to investigate related signaling pathways. Of note, inhibition, knockdown and upregulation of the membrane protein Chloride intracellular channel 1 (CLIC1) can affect GBC resistance in the presence of metformin. Our data demonstrated that metformin apparently inhibits the proliferation and viability of GBC cells. Metformin promoted cell apoptosis and increased the number of early apoptotic cells. We found that metformin can exert growth‐suppressive effects on these cell lines via inhibition of p‐Akt activity and the Bcl‐2 family. Notably, either dysfunction or downregulation of CLIC1 can partially decrease the antineoplastic effects of metformin while upregulation of CLIC1 can increase drug sensitivity. Our findings provide experimental evidence for using metformin as an antitumor treatment for gallbladder carcinoma. John Wiley and Sons Inc. 2017-05-22 2017-06 /pmc/articles/PMC5480064/ /pubmed/28378944 http://dx.doi.org/10.1111/cas.13248 Text en © 2017 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association. This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial (http://creativecommons.org/licenses/by-nc/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. |
spellingShingle | Original Articles Liu, Yongchen Wang, Zheng Li, Maolan Ye, Yuanyuan Xu, Yi Zhang, Yichi Yuan, Ruiyan Jin, Yunpeng Hao, Yajuan Jiang, Lin Hu, Yunping Chen, Shili Liu, Fatao Zhang, Yijian Wu, Wenguang Liu, Yingbin Chloride intracellular channel 1 regulates the antineoplastic effects of metformin in gallbladder cancer cells |
title | Chloride intracellular channel 1 regulates the antineoplastic effects of metformin in gallbladder cancer cells |
title_full | Chloride intracellular channel 1 regulates the antineoplastic effects of metformin in gallbladder cancer cells |
title_fullStr | Chloride intracellular channel 1 regulates the antineoplastic effects of metformin in gallbladder cancer cells |
title_full_unstemmed | Chloride intracellular channel 1 regulates the antineoplastic effects of metformin in gallbladder cancer cells |
title_short | Chloride intracellular channel 1 regulates the antineoplastic effects of metformin in gallbladder cancer cells |
title_sort | chloride intracellular channel 1 regulates the antineoplastic effects of metformin in gallbladder cancer cells |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5480064/ https://www.ncbi.nlm.nih.gov/pubmed/28378944 http://dx.doi.org/10.1111/cas.13248 |
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