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A Highly Efficient CRISPR-Cas9-Mediated Large Genomic Deletion in Bacillus subtilis

In Bacillus subtilis, large genomic deletions have been carried out for genome reduction, antibiotic overproduction, and heterologous protein overexpression. In view of the eco-friendliness of B. subtilis, it is critical that engineering preserves its food-grade status and avoids leaving foreign DNA...

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Detalles Bibliográficos
Autores principales:	So, Younju, Park, Soo-Young, Park, Eun-Hye, Park, Seung-Hwan, Kim, Eui-Joong, Pan, Jae-Gu, Choi, Soo-Keun
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2017
Materias:	Microbiology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5481315/ https://www.ncbi.nlm.nih.gov/pubmed/28690606 http://dx.doi.org/10.3389/fmicb.2017.01167

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