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CRISPR/Cas9-mediated gene knockout screens and target identification via whole-genome sequencing uncover host genes required for picornavirus infection

Several groups have used genome-wide libraries of lentiviruses encoding small guide RNAs (sgRNAs) for genetic screens. In most cases, sgRNA expression cassettes are integrated into cells by using lentiviruses, and target genes are statistically estimated by the readout of sgRNA sequences after targe...

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Detalles Bibliográficos
Autores principales:	Kim, Heon Seok, Lee, Kyungjin, Bae, Sangsu, Park, Jeongbin, Lee, Chong-Kyo, Kim, Meehyein, Kim, Eunji, Kim, Minju, Kim, Seokjoong, Kim, Chonsaeng, Kim, Jin-Soo
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Society for Biochemistry and Molecular Biology 2017
Materias:	Cell Biology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5481571/ https://www.ncbi.nlm.nih.gov/pubmed/28446605 http://dx.doi.org/10.1074/jbc.M117.782425

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