Inhibition of casein kinase 2 blocks G(2)/M transition in early embryo mitosis but not in oocyte meiosis in mouse

Casein kinase 2 (CK2) is a highly conserved, ubiquitously expressed serine/threonine protein kinase with hundreds of substrates. The role of CK2 in the G(2)/M transition of oocytes, zygotes, and 2-cell embryos was studied in mouse by enzyme activity inhibition using the specific inhibitor 4, 5, 6, 7-tetrabromobenzotriazole (TBB). Zygotes and 2-cell embryos were arrested at G(2) phase by TBB treatment, and DNA damage was increased in the female pronucleus of arrested zygotes. Further developmental ability of arrested zygotes was reduced, but that of arrested 2-cell embryos was not affected after releasing from inhibition. By contrast, the G(2)/M transition in oocytes was not affected by TBB. These results indicate that CK2 activity is essential for mitotic G(2)/M transition in early embryos but not for meiotic G(2)/M transition in oocytes.