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Pu-18-N-butylimide-NMGA-GNP conjugate is effective against hepatocellular carcinoma
BACKGROUND: Photodynamic therapy (PDT) is a new modality in the treatment of cancer. This study thus aims to examine whether the PDT is effective against in vivo hepatocellular carcinoma. METHODS: In vivo efficacy of PDT on hepatocellular carcinoma was tested in xenografted mice with human hepatocel...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5481715/ https://www.ncbi.nlm.nih.gov/pubmed/28664061 http://dx.doi.org/10.1016/j.imr.2013.05.001 |
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author | Kwon, Jin-Geun Song, In-Sung Kim, Min-Soo Lee, Beom Hee Kim, Jung Hwa Yoon, Il Shim, Young Key Kim, Nari Han, Jin Youm, Jae Boum |
author_facet | Kwon, Jin-Geun Song, In-Sung Kim, Min-Soo Lee, Beom Hee Kim, Jung Hwa Yoon, Il Shim, Young Key Kim, Nari Han, Jin Youm, Jae Boum |
author_sort | Kwon, Jin-Geun |
collection | PubMed |
description | BACKGROUND: Photodynamic therapy (PDT) is a new modality in the treatment of cancer. This study thus aims to examine whether the PDT is effective against in vivo hepatocellular carcinoma. METHODS: In vivo efficacy of PDT on hepatocellular carcinoma was tested in xenografted mice with human hepatocellular carcinoma cell lines (Huh7) by utilizing a gold nanoparticles (GNPs) conjugate of new photosensitizer (PS), purpurin-18-N-butylimide-N-methyl-D-glucamine (Pu-18-N-butylimide-NMGA). The conjugate (PS-GNPs) was synthesized from the reaction between Pu-18-N-butylimide-NMGA and chloroauric acid (HAuCl4). Mice were arbitrarily assigned into one of three groups. First group received saline alone, second group received PS-GNPs alone, and the last group received both PS-GNPs and irradiation. PS-GNPs was injected directly into the tumor mass and irradiations were performed 24 hours after injection of PS-GNPs. RESULTS: Tumor volume was significantly smaller in the group which received both PS-GNPs and irradiation compared with other two groups. Western blot and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay revealed that the group which received both PS-GNPs and irradiation showed larger amount of apoptotic protein and DNA fragmentation compared with other two groups. CONCLUSION: This study suggests that Pu-18-N-butylimide-NMGA-GNP conjugate is an effective agent for PDT in the treatment of hepatocellular carcinoma. |
format | Online Article Text |
id | pubmed-5481715 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-54817152017-06-29 Pu-18-N-butylimide-NMGA-GNP conjugate is effective against hepatocellular carcinoma Kwon, Jin-Geun Song, In-Sung Kim, Min-Soo Lee, Beom Hee Kim, Jung Hwa Yoon, Il Shim, Young Key Kim, Nari Han, Jin Youm, Jae Boum Integr Med Res Original Article BACKGROUND: Photodynamic therapy (PDT) is a new modality in the treatment of cancer. This study thus aims to examine whether the PDT is effective against in vivo hepatocellular carcinoma. METHODS: In vivo efficacy of PDT on hepatocellular carcinoma was tested in xenografted mice with human hepatocellular carcinoma cell lines (Huh7) by utilizing a gold nanoparticles (GNPs) conjugate of new photosensitizer (PS), purpurin-18-N-butylimide-N-methyl-D-glucamine (Pu-18-N-butylimide-NMGA). The conjugate (PS-GNPs) was synthesized from the reaction between Pu-18-N-butylimide-NMGA and chloroauric acid (HAuCl4). Mice were arbitrarily assigned into one of three groups. First group received saline alone, second group received PS-GNPs alone, and the last group received both PS-GNPs and irradiation. PS-GNPs was injected directly into the tumor mass and irradiations were performed 24 hours after injection of PS-GNPs. RESULTS: Tumor volume was significantly smaller in the group which received both PS-GNPs and irradiation compared with other two groups. Western blot and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay revealed that the group which received both PS-GNPs and irradiation showed larger amount of apoptotic protein and DNA fragmentation compared with other two groups. CONCLUSION: This study suggests that Pu-18-N-butylimide-NMGA-GNP conjugate is an effective agent for PDT in the treatment of hepatocellular carcinoma. Elsevier 2013-09 2013-06-13 /pmc/articles/PMC5481715/ /pubmed/28664061 http://dx.doi.org/10.1016/j.imr.2013.05.001 Text en © 2013 Korea Institute of Oriental Medicine. Published by Elsevier. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Original Article Kwon, Jin-Geun Song, In-Sung Kim, Min-Soo Lee, Beom Hee Kim, Jung Hwa Yoon, Il Shim, Young Key Kim, Nari Han, Jin Youm, Jae Boum Pu-18-N-butylimide-NMGA-GNP conjugate is effective against hepatocellular carcinoma |
title | Pu-18-N-butylimide-NMGA-GNP conjugate is effective against hepatocellular carcinoma |
title_full | Pu-18-N-butylimide-NMGA-GNP conjugate is effective against hepatocellular carcinoma |
title_fullStr | Pu-18-N-butylimide-NMGA-GNP conjugate is effective against hepatocellular carcinoma |
title_full_unstemmed | Pu-18-N-butylimide-NMGA-GNP conjugate is effective against hepatocellular carcinoma |
title_short | Pu-18-N-butylimide-NMGA-GNP conjugate is effective against hepatocellular carcinoma |
title_sort | pu-18-n-butylimide-nmga-gnp conjugate is effective against hepatocellular carcinoma |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5481715/ https://www.ncbi.nlm.nih.gov/pubmed/28664061 http://dx.doi.org/10.1016/j.imr.2013.05.001 |
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