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Inhibitory effects of Taraxacum mongolicum with phreatic water on melanin synthesis

BACKGROUND: Recently, people have begun showing heightened interest in skin whitening. Melanin is an important factor that determines skin color. The purpose of this study is to investigate the inhibitory effect of Taraxacum mongolicum (TAM) with phreatic water (PW) from Dogo Hot Springs on melanin...

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Autores principales: Jang, Moon-Hee, Ahn, Taek-Won
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5481772/
https://www.ncbi.nlm.nih.gov/pubmed/28664113
http://dx.doi.org/10.1016/j.imr.2014.08.001
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author Jang, Moon-Hee
Ahn, Taek-Won
author_facet Jang, Moon-Hee
Ahn, Taek-Won
author_sort Jang, Moon-Hee
collection PubMed
description BACKGROUND: Recently, people have begun showing heightened interest in skin whitening. Melanin is an important factor that determines skin color. The purpose of this study is to investigate the inhibitory effect of Taraxacum mongolicum (TAM) with phreatic water (PW) from Dogo Hot Springs on melanin synthesis. METHODS: We assessed the inhibitory effects of TAM on melanin synthesis in B16F10 mouse melanoma cells. The mRNA levels of tyrosinase related protein (TRP)-1, TRP-2, tyrosinase, MITF, ERK, and PKA protein were analyzed with reverse transcription polymerase chain reaction and Western blot analysis. We also assessed the inhibitory effects of TAM with PW on melanin synthesis in HRM-2 melanin-possessing hairless mice. After UVB irradiation, differences in melanin were analyzed with an image analysis software between the left dorsal skin (untreated part) and the right dorsal skin (treated part). The mRNA levels of TRP-1, TRP-2, and matrix metalloproteinase (MMP)-9 were analyzed with real-time quantitative polymerase chain reaction. The dorsal skins were analyzed with histological test by hematoxylin and eosin staining. RESULTS: TAM inhibited the TRP-1, TRP-2, tyrosinase, MITF mRNA gene expression, and PKA protein expression on the concentration-dependent B16F10 cell. Moreover, TAM increased the ERK mRNA gene expression in the B16F10 cell. After UVB irradiation, TAM with PW increased the differences in melanin between the left dorsal skin (untreated part) and the right dorsal skin (treated part) in HRM-2 mice. TAM with PW inhibited the TRP-1, TRP-2, and MMP-9 mRNA gene expression in HRM-2 mice. TAM with PW decreased the epidermal thickness, around the cell deformation, keratinization, and infiltration in HRM-2 mice. CONCLUSION: These results indicate that TAM with PW has the inhibitory effect of decreasing the melanin synthesis.
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spelling pubmed-54817722017-06-29 Inhibitory effects of Taraxacum mongolicum with phreatic water on melanin synthesis Jang, Moon-Hee Ahn, Taek-Won Integr Med Res Original Article BACKGROUND: Recently, people have begun showing heightened interest in skin whitening. Melanin is an important factor that determines skin color. The purpose of this study is to investigate the inhibitory effect of Taraxacum mongolicum (TAM) with phreatic water (PW) from Dogo Hot Springs on melanin synthesis. METHODS: We assessed the inhibitory effects of TAM on melanin synthesis in B16F10 mouse melanoma cells. The mRNA levels of tyrosinase related protein (TRP)-1, TRP-2, tyrosinase, MITF, ERK, and PKA protein were analyzed with reverse transcription polymerase chain reaction and Western blot analysis. We also assessed the inhibitory effects of TAM with PW on melanin synthesis in HRM-2 melanin-possessing hairless mice. After UVB irradiation, differences in melanin were analyzed with an image analysis software between the left dorsal skin (untreated part) and the right dorsal skin (treated part). The mRNA levels of TRP-1, TRP-2, and matrix metalloproteinase (MMP)-9 were analyzed with real-time quantitative polymerase chain reaction. The dorsal skins were analyzed with histological test by hematoxylin and eosin staining. RESULTS: TAM inhibited the TRP-1, TRP-2, tyrosinase, MITF mRNA gene expression, and PKA protein expression on the concentration-dependent B16F10 cell. Moreover, TAM increased the ERK mRNA gene expression in the B16F10 cell. After UVB irradiation, TAM with PW increased the differences in melanin between the left dorsal skin (untreated part) and the right dorsal skin (treated part) in HRM-2 mice. TAM with PW inhibited the TRP-1, TRP-2, and MMP-9 mRNA gene expression in HRM-2 mice. TAM with PW decreased the epidermal thickness, around the cell deformation, keratinization, and infiltration in HRM-2 mice. CONCLUSION: These results indicate that TAM with PW has the inhibitory effect of decreasing the melanin synthesis. Elsevier 2015-06 2014-08-30 /pmc/articles/PMC5481772/ /pubmed/28664113 http://dx.doi.org/10.1016/j.imr.2014.08.001 Text en © 2014 Korea Institute of Oriental Medicine. Published by Elsevier. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Jang, Moon-Hee
Ahn, Taek-Won
Inhibitory effects of Taraxacum mongolicum with phreatic water on melanin synthesis
title Inhibitory effects of Taraxacum mongolicum with phreatic water on melanin synthesis
title_full Inhibitory effects of Taraxacum mongolicum with phreatic water on melanin synthesis
title_fullStr Inhibitory effects of Taraxacum mongolicum with phreatic water on melanin synthesis
title_full_unstemmed Inhibitory effects of Taraxacum mongolicum with phreatic water on melanin synthesis
title_short Inhibitory effects of Taraxacum mongolicum with phreatic water on melanin synthesis
title_sort inhibitory effects of taraxacum mongolicum with phreatic water on melanin synthesis
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5481772/
https://www.ncbi.nlm.nih.gov/pubmed/28664113
http://dx.doi.org/10.1016/j.imr.2014.08.001
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