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Pre-treatment with Chrysanthemum indicum Linné extract protects pyramidal neurons from transient cerebral ischemia via increasing antioxidants in the gerbil hippocampal CA1 region

Chrysanthemum indicum Linné extract (CIL) is used in herbal medicine in East Asia. In the present study, gerbils were orally pre-treated with CIL, and changes of antioxidant enzymes including superoxide dismutase (SOD) 1 and SOD2, catalase (CAT) and glutathione peroxidase (GPX) in the hippocampal CA...

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Autores principales: Kim, In Hye, Lee, Tae-Kyeong, Cho, Jeong Hwi, Lee, Jae-Chul, Park, Joon Ha, Ahn, Ji Hyeon, Shin, Bich-Na, Chen, Bai Hui, Tae, Hyun-Jin, Kim, Yang Hee, Kim, Jong-Dai, Kim, Young-Myeong, Won, Moo-Ho, Kang, Il Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5482094/
https://www.ncbi.nlm.nih.gov/pubmed/28534982
http://dx.doi.org/10.3892/mmr.2017.6591
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author Kim, In Hye
Lee, Tae-Kyeong
Cho, Jeong Hwi
Lee, Jae-Chul
Park, Joon Ha
Ahn, Ji Hyeon
Shin, Bich-Na
Chen, Bai Hui
Tae, Hyun-Jin
Kim, Yang Hee
Kim, Jong-Dai
Kim, Young-Myeong
Won, Moo-Ho
Kang, Il Jun
author_facet Kim, In Hye
Lee, Tae-Kyeong
Cho, Jeong Hwi
Lee, Jae-Chul
Park, Joon Ha
Ahn, Ji Hyeon
Shin, Bich-Na
Chen, Bai Hui
Tae, Hyun-Jin
Kim, Yang Hee
Kim, Jong-Dai
Kim, Young-Myeong
Won, Moo-Ho
Kang, Il Jun
author_sort Kim, In Hye
collection PubMed
description Chrysanthemum indicum Linné extract (CIL) is used in herbal medicine in East Asia. In the present study, gerbils were orally pre-treated with CIL, and changes of antioxidant enzymes including superoxide dismutase (SOD) 1 and SOD2, catalase (CAT) and glutathione peroxidase (GPX) in the hippocampal CA1 region following 5 min of transient cerebral ischemia were investigated and the neuroprotective effect of CIL in the ischemic CA1 region was examined. SOD1, SOD2, CAT and GPX immunoreactivities were observed in the pyramidal cells of the CA1 region and their immunoreactivities were gradually decreased following ischemia-reperfusion and barely detectable at 5 days post-ischemia. CIL pre-treatment significantly increased immunoreactivities of SOD1, CAT and GPX, but not SOD2, in the CA1 pyramidal cells of the sham-operated animals. In addition, SOD1, SOD2, CAT and GPX immunoreactivities in the CA1 pyramidal cells were significantly higher compared with the ischemia-operated animals. Furthermore, it was identified that pre-treatment with CIL protected the CA1 pyramidal cells in the CA1 region using neuronal nuclei immunohistochemistry and Fluoro-Jade B histofluorescence staining; the protected CA1 pyramidal cells were 67.5% compared with the sham-operated animals. In conclusion, oral CIL pre-treatment increased endogenous antioxidant enzymes in CA1 pyramidal cells in the gerbil hippocampus and protected the cells from transient cerebral ischemic insult. This finding suggested that CIL is promising for the prevention of ischemia-induced neuronal damage.
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spelling pubmed-54820942017-06-28 Pre-treatment with Chrysanthemum indicum Linné extract protects pyramidal neurons from transient cerebral ischemia via increasing antioxidants in the gerbil hippocampal CA1 region Kim, In Hye Lee, Tae-Kyeong Cho, Jeong Hwi Lee, Jae-Chul Park, Joon Ha Ahn, Ji Hyeon Shin, Bich-Na Chen, Bai Hui Tae, Hyun-Jin Kim, Yang Hee Kim, Jong-Dai Kim, Young-Myeong Won, Moo-Ho Kang, Il Jun Mol Med Rep Articles Chrysanthemum indicum Linné extract (CIL) is used in herbal medicine in East Asia. In the present study, gerbils were orally pre-treated with CIL, and changes of antioxidant enzymes including superoxide dismutase (SOD) 1 and SOD2, catalase (CAT) and glutathione peroxidase (GPX) in the hippocampal CA1 region following 5 min of transient cerebral ischemia were investigated and the neuroprotective effect of CIL in the ischemic CA1 region was examined. SOD1, SOD2, CAT and GPX immunoreactivities were observed in the pyramidal cells of the CA1 region and their immunoreactivities were gradually decreased following ischemia-reperfusion and barely detectable at 5 days post-ischemia. CIL pre-treatment significantly increased immunoreactivities of SOD1, CAT and GPX, but not SOD2, in the CA1 pyramidal cells of the sham-operated animals. In addition, SOD1, SOD2, CAT and GPX immunoreactivities in the CA1 pyramidal cells were significantly higher compared with the ischemia-operated animals. Furthermore, it was identified that pre-treatment with CIL protected the CA1 pyramidal cells in the CA1 region using neuronal nuclei immunohistochemistry and Fluoro-Jade B histofluorescence staining; the protected CA1 pyramidal cells were 67.5% compared with the sham-operated animals. In conclusion, oral CIL pre-treatment increased endogenous antioxidant enzymes in CA1 pyramidal cells in the gerbil hippocampus and protected the cells from transient cerebral ischemic insult. This finding suggested that CIL is promising for the prevention of ischemia-induced neuronal damage. D.A. Spandidos 2017-07 2017-05-17 /pmc/articles/PMC5482094/ /pubmed/28534982 http://dx.doi.org/10.3892/mmr.2017.6591 Text en Copyright: © Kim et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Kim, In Hye
Lee, Tae-Kyeong
Cho, Jeong Hwi
Lee, Jae-Chul
Park, Joon Ha
Ahn, Ji Hyeon
Shin, Bich-Na
Chen, Bai Hui
Tae, Hyun-Jin
Kim, Yang Hee
Kim, Jong-Dai
Kim, Young-Myeong
Won, Moo-Ho
Kang, Il Jun
Pre-treatment with Chrysanthemum indicum Linné extract protects pyramidal neurons from transient cerebral ischemia via increasing antioxidants in the gerbil hippocampal CA1 region
title Pre-treatment with Chrysanthemum indicum Linné extract protects pyramidal neurons from transient cerebral ischemia via increasing antioxidants in the gerbil hippocampal CA1 region
title_full Pre-treatment with Chrysanthemum indicum Linné extract protects pyramidal neurons from transient cerebral ischemia via increasing antioxidants in the gerbil hippocampal CA1 region
title_fullStr Pre-treatment with Chrysanthemum indicum Linné extract protects pyramidal neurons from transient cerebral ischemia via increasing antioxidants in the gerbil hippocampal CA1 region
title_full_unstemmed Pre-treatment with Chrysanthemum indicum Linné extract protects pyramidal neurons from transient cerebral ischemia via increasing antioxidants in the gerbil hippocampal CA1 region
title_short Pre-treatment with Chrysanthemum indicum Linné extract protects pyramidal neurons from transient cerebral ischemia via increasing antioxidants in the gerbil hippocampal CA1 region
title_sort pre-treatment with chrysanthemum indicum linné extract protects pyramidal neurons from transient cerebral ischemia via increasing antioxidants in the gerbil hippocampal ca1 region
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5482094/
https://www.ncbi.nlm.nih.gov/pubmed/28534982
http://dx.doi.org/10.3892/mmr.2017.6591
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