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Investigations of the prevalence and virulence of Candida albicans in periodontal and endodontic lesions in diabetic and normoglycemic patients

Pulpal and periodontal tissues have similar microbiota that allows cross-contamination between the pulp and periodontal tissues. OBJECTIVE: The aim of this study was to investigate the prevalence of isolated Candida albicans from periodontal endodontic lesions in diabetic and normoglycemic patients,...

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Autores principales: GOMES, Cinthya Cristina, GUIMARÃES, Ludmila Silva, PINTO, Larissa Christina Costa, CAMARGO, Gabriela Alessandra da Cruz Galhardo, VALENTE, Maria Isabel Bastos, SARQUIS, Maria Inêz de Moura
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Faculdade De Odontologia De Bauru - USP 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5482250/
https://www.ncbi.nlm.nih.gov/pubmed/28678946
http://dx.doi.org/10.1590/1678-7757-2016-0432
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author GOMES, Cinthya Cristina
GUIMARÃES, Ludmila Silva
PINTO, Larissa Christina Costa
CAMARGO, Gabriela Alessandra da Cruz Galhardo
VALENTE, Maria Isabel Bastos
SARQUIS, Maria Inêz de Moura
author_facet GOMES, Cinthya Cristina
GUIMARÃES, Ludmila Silva
PINTO, Larissa Christina Costa
CAMARGO, Gabriela Alessandra da Cruz Galhardo
VALENTE, Maria Isabel Bastos
SARQUIS, Maria Inêz de Moura
author_sort GOMES, Cinthya Cristina
collection PubMed
description Pulpal and periodontal tissues have similar microbiota that allows cross-contamination between the pulp and periodontal tissues. OBJECTIVE: The aim of this study was to investigate the prevalence of isolated Candida albicans from periodontal endodontic lesions in diabetic and normoglycemic patients, and the fungi's virulence in different atmospheric conditions. MATERIAL AND METHODS: A case-control study was conducted on 15 patients with type 2 diabetes mellitus (G1) and 15 non-diabetics (G2) with periodontal endodontic lesions. Samples of root canals and periodontal pockets were plated on CHROMagar for later identification by polymerase chain reaction (PCR) and virulence test. RESULTS: C. albicans was identified in 79.2% and 20.8% of the 60 samples collected from diabetic and normoglycemic patients, respectively. Of the 30 samples collected from periodontal pockets, 13 showed a positive culture for C. albicans, with 77% belonging to G1 and 23% to G2. Of the 11 positive samples from root canals, 82% were from G1 and 18% from G2. Production of proteinase presented a precipitation zone Pz<0.63 of 100% in G1 and 72% in G2, in redox and negative (Pz=1), under anaerobic conditions in both groups. Hydrophobicity of the strains from G1 indicated 16.4% with low, 19.3% with moderate, and 64.3% with high hydrophobicity in redox. In G2, 42.2% had low, 39.8% had moderate, 18% had high hydrophobicity in redox. In anaerobic conditions, G1 showed 15.2% with low, 12.8% with moderate, and 72% with high hydrophobicity; in G2, 33.6% had low, 28.8% had moderate, and 37.6% had high hydrophobicity. There was statistical difference in the number of positive cultures between G1 and G2 (p<0.05) with predominance in G1. There was statistical difference for all virulence factors, except hemolysis (p=0.001). CONCLUSIONS: Candida albicans was isolated more frequently and had higher virulence in diabetic patients.
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spelling pubmed-54822502017-06-28 Investigations of the prevalence and virulence of Candida albicans in periodontal and endodontic lesions in diabetic and normoglycemic patients GOMES, Cinthya Cristina GUIMARÃES, Ludmila Silva PINTO, Larissa Christina Costa CAMARGO, Gabriela Alessandra da Cruz Galhardo VALENTE, Maria Isabel Bastos SARQUIS, Maria Inêz de Moura J Appl Oral Sci Original Articles Pulpal and periodontal tissues have similar microbiota that allows cross-contamination between the pulp and periodontal tissues. OBJECTIVE: The aim of this study was to investigate the prevalence of isolated Candida albicans from periodontal endodontic lesions in diabetic and normoglycemic patients, and the fungi's virulence in different atmospheric conditions. MATERIAL AND METHODS: A case-control study was conducted on 15 patients with type 2 diabetes mellitus (G1) and 15 non-diabetics (G2) with periodontal endodontic lesions. Samples of root canals and periodontal pockets were plated on CHROMagar for later identification by polymerase chain reaction (PCR) and virulence test. RESULTS: C. albicans was identified in 79.2% and 20.8% of the 60 samples collected from diabetic and normoglycemic patients, respectively. Of the 30 samples collected from periodontal pockets, 13 showed a positive culture for C. albicans, with 77% belonging to G1 and 23% to G2. Of the 11 positive samples from root canals, 82% were from G1 and 18% from G2. Production of proteinase presented a precipitation zone Pz<0.63 of 100% in G1 and 72% in G2, in redox and negative (Pz=1), under anaerobic conditions in both groups. Hydrophobicity of the strains from G1 indicated 16.4% with low, 19.3% with moderate, and 64.3% with high hydrophobicity in redox. In G2, 42.2% had low, 39.8% had moderate, 18% had high hydrophobicity in redox. In anaerobic conditions, G1 showed 15.2% with low, 12.8% with moderate, and 72% with high hydrophobicity; in G2, 33.6% had low, 28.8% had moderate, and 37.6% had high hydrophobicity. There was statistical difference in the number of positive cultures between G1 and G2 (p<0.05) with predominance in G1. There was statistical difference for all virulence factors, except hemolysis (p=0.001). CONCLUSIONS: Candida albicans was isolated more frequently and had higher virulence in diabetic patients. Faculdade De Odontologia De Bauru - USP 2017 /pmc/articles/PMC5482250/ /pubmed/28678946 http://dx.doi.org/10.1590/1678-7757-2016-0432 Text en http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
GOMES, Cinthya Cristina
GUIMARÃES, Ludmila Silva
PINTO, Larissa Christina Costa
CAMARGO, Gabriela Alessandra da Cruz Galhardo
VALENTE, Maria Isabel Bastos
SARQUIS, Maria Inêz de Moura
Investigations of the prevalence and virulence of Candida albicans in periodontal and endodontic lesions in diabetic and normoglycemic patients
title Investigations of the prevalence and virulence of Candida albicans in periodontal and endodontic lesions in diabetic and normoglycemic patients
title_full Investigations of the prevalence and virulence of Candida albicans in periodontal and endodontic lesions in diabetic and normoglycemic patients
title_fullStr Investigations of the prevalence and virulence of Candida albicans in periodontal and endodontic lesions in diabetic and normoglycemic patients
title_full_unstemmed Investigations of the prevalence and virulence of Candida albicans in periodontal and endodontic lesions in diabetic and normoglycemic patients
title_short Investigations of the prevalence and virulence of Candida albicans in periodontal and endodontic lesions in diabetic and normoglycemic patients
title_sort investigations of the prevalence and virulence of candida albicans in periodontal and endodontic lesions in diabetic and normoglycemic patients
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5482250/
https://www.ncbi.nlm.nih.gov/pubmed/28678946
http://dx.doi.org/10.1590/1678-7757-2016-0432
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