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High expression of PTBP1 promote invasion of colorectal cancer by alternative splicing of cortactin
Polypyrimidine tract-binding protein 1 (PTBP1) involving in almost all steps of mRNA regulation including alternative splicing metabolism during tumorigenesis due to its RNA-binding activity. Initially, we found that high expressed PTBP1 and poor prognosis was interrelated in colorectal cancer (CRC)...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5482648/ https://www.ncbi.nlm.nih.gov/pubmed/28404950 http://dx.doi.org/10.18632/oncotarget.15873 |
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author | Wang, Zhi-na Liu, Dan Yin, Bin Ju, Wen-yi Qiu, Hui-zhong Xiao, Yi Chen, Yuan-jia Peng, Xiao-zhong Lu, Chong-mei |
author_facet | Wang, Zhi-na Liu, Dan Yin, Bin Ju, Wen-yi Qiu, Hui-zhong Xiao, Yi Chen, Yuan-jia Peng, Xiao-zhong Lu, Chong-mei |
author_sort | Wang, Zhi-na |
collection | PubMed |
description | Polypyrimidine tract-binding protein 1 (PTBP1) involving in almost all steps of mRNA regulation including alternative splicing metabolism during tumorigenesis due to its RNA-binding activity. Initially, we found that high expressed PTBP1 and poor prognosis was interrelated in colorectal cancer (CRC) patients with stages II and III CRC, which widely different in prognosis and treatment, by immunohistochemistry. PTBP1 was also upregulated in colon cancer cell lines. In our study, knockdown of PTBP1 by siRNA transfection decreased cell proliferation and invasion in vitro. Denovirus shRNA knockdown of PTBP1 inhibited colorectal cancer growth in vivo. Furthermore, PTBP1 regulates alternative splicing of many target genes involving in tumorgenesis in colon cancer cells. We confirmed that the splicing of cortactin exon 11 which was only contained in cortactin isoform-a, as a PTBP1 target. Knockdown of PTBP1 decreased the expression of cortactin isoform-a by exclusion of exon 11. Also the mRNA levels of PTBP1 and cortactin isoform-a were cooperatively expressed in colorectal cancer tissues. Knocking down cortactin isoform-a significantly decreased cell migration and invasion in colorectal cancer cells. Overexpression of cortactin isoform-a could rescue PTBP1-knockdown effect of cell motility. In summary the study revealed that PTBP1 facilitates colorectal cancer migration and invasion activities by inclusion of cortactin exon 11. |
format | Online Article Text |
id | pubmed-5482648 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-54826482017-06-27 High expression of PTBP1 promote invasion of colorectal cancer by alternative splicing of cortactin Wang, Zhi-na Liu, Dan Yin, Bin Ju, Wen-yi Qiu, Hui-zhong Xiao, Yi Chen, Yuan-jia Peng, Xiao-zhong Lu, Chong-mei Oncotarget Research Paper Polypyrimidine tract-binding protein 1 (PTBP1) involving in almost all steps of mRNA regulation including alternative splicing metabolism during tumorigenesis due to its RNA-binding activity. Initially, we found that high expressed PTBP1 and poor prognosis was interrelated in colorectal cancer (CRC) patients with stages II and III CRC, which widely different in prognosis and treatment, by immunohistochemistry. PTBP1 was also upregulated in colon cancer cell lines. In our study, knockdown of PTBP1 by siRNA transfection decreased cell proliferation and invasion in vitro. Denovirus shRNA knockdown of PTBP1 inhibited colorectal cancer growth in vivo. Furthermore, PTBP1 regulates alternative splicing of many target genes involving in tumorgenesis in colon cancer cells. We confirmed that the splicing of cortactin exon 11 which was only contained in cortactin isoform-a, as a PTBP1 target. Knockdown of PTBP1 decreased the expression of cortactin isoform-a by exclusion of exon 11. Also the mRNA levels of PTBP1 and cortactin isoform-a were cooperatively expressed in colorectal cancer tissues. Knocking down cortactin isoform-a significantly decreased cell migration and invasion in colorectal cancer cells. Overexpression of cortactin isoform-a could rescue PTBP1-knockdown effect of cell motility. In summary the study revealed that PTBP1 facilitates colorectal cancer migration and invasion activities by inclusion of cortactin exon 11. Impact Journals LLC 2017-03-03 /pmc/articles/PMC5482648/ /pubmed/28404950 http://dx.doi.org/10.18632/oncotarget.15873 Text en Copyright: © 2017 Wang et al. http://creativecommons.org/licenses/by/3.0/ This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/) (CC-BY), which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Research Paper Wang, Zhi-na Liu, Dan Yin, Bin Ju, Wen-yi Qiu, Hui-zhong Xiao, Yi Chen, Yuan-jia Peng, Xiao-zhong Lu, Chong-mei High expression of PTBP1 promote invasion of colorectal cancer by alternative splicing of cortactin |
title | High expression of PTBP1 promote invasion of colorectal cancer by alternative splicing of cortactin |
title_full | High expression of PTBP1 promote invasion of colorectal cancer by alternative splicing of cortactin |
title_fullStr | High expression of PTBP1 promote invasion of colorectal cancer by alternative splicing of cortactin |
title_full_unstemmed | High expression of PTBP1 promote invasion of colorectal cancer by alternative splicing of cortactin |
title_short | High expression of PTBP1 promote invasion of colorectal cancer by alternative splicing of cortactin |
title_sort | high expression of ptbp1 promote invasion of colorectal cancer by alternative splicing of cortactin |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5482648/ https://www.ncbi.nlm.nih.gov/pubmed/28404950 http://dx.doi.org/10.18632/oncotarget.15873 |
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