Cargando…
In vivo characterization of abnormalities in small-bowel diseases using probe-based confocal laser endomicroscopy
BACKGROUND AND STUDY AIMS: Probe-based confocal laser endomicroscopy (pCLE) enables real-time optical biopsy. Little is known about pCLE imaging deep inside the small bowel, therefore the aim of this study was to determine its usefulness. PATIENTS AND METHODS: Between April 2014 and January 2016,...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
© Georg Thieme Verlag KG
2017
|
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5482742/ https://www.ncbi.nlm.nih.gov/pubmed/28670610 http://dx.doi.org/10.1055/s-0043-106184 |
_version_ | 1783245624950390784 |
---|---|
author | Ohmiya, Naoki Horiguchi, Noriyuki Tahara, Tomomitsu Nagasaka, Mitsuo Nakagawa, Yoshihito Shibata, Tomoyuki Tsukamoto, Tetsuya Kuroda, Makoto |
author_facet | Ohmiya, Naoki Horiguchi, Noriyuki Tahara, Tomomitsu Nagasaka, Mitsuo Nakagawa, Yoshihito Shibata, Tomoyuki Tsukamoto, Tetsuya Kuroda, Makoto |
author_sort | Ohmiya, Naoki |
collection | PubMed |
description | BACKGROUND AND STUDY AIMS: Probe-based confocal laser endomicroscopy (pCLE) enables real-time optical biopsy. Little is known about pCLE imaging deep inside the small bowel, therefore the aim of this study was to determine its usefulness. PATIENTS AND METHODS: Between April 2014 and January 2016, we performed 38 pCLE examinations during double-balloon enteroscopy with intravenous fluorescein in 37 patients with: tumors (n = 10), vascular disorders (n = 6), inflammatory diseases and drug injuries (n = 13), other disorders (n = 4), and normal findings (n = 4). We measured the calibers of capillary and lymphatic vessels at 15 different sites and compared the calibers between pCLE images and histopathology. We also compared pCLE findings with pathologic diagnosis. RESULTS: The inner diameters of capillary vessels beneath the epithelium and in the middle of villi were 16.2 ± 3.0 µm and 14.5 ± 3.1 µm, respectively, in the pCLE images, but these were not consistent with formalin-fixed paraffin-embedded histology. In tumors, larger capillary vessels were observed in aggressive malignant lymphoma and metastasis, and a “soccer ball-like pattern” constituting homogenous dark cells packed with polygonal, narrower capillary vessels was characteristic in pCLE images of a malignant lymphoma follicle. Hereditary hemorrhagic telangiectasia and angiodysplasia contained anastomosis of capillary vessels of different calibers. In IgA vasculitis, segmental capillary strictures were observed. Intestinal lymphangiectasia with protein-losing enteropathy contained a reticular network of lymphatic vessels and dilated lymphatic ducts accompanied by numerous cell gaps. pCLE findings corresponded to pathologic diagnosis in 32 examinations (91 %). CONCLUSIONS: pCLE is useful for in vivo analysis of abnormalities of the capillary and lymphatic vessels and epithelium, and for diagnosis in various small-bowel diseases. |
format | Online Article Text |
id | pubmed-5482742 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | © Georg Thieme Verlag KG |
record_format | MEDLINE/PubMed |
spelling | pubmed-54827422017-07-01 In vivo characterization of abnormalities in small-bowel diseases using probe-based confocal laser endomicroscopy Ohmiya, Naoki Horiguchi, Noriyuki Tahara, Tomomitsu Nagasaka, Mitsuo Nakagawa, Yoshihito Shibata, Tomoyuki Tsukamoto, Tetsuya Kuroda, Makoto Endosc Int Open BACKGROUND AND STUDY AIMS: Probe-based confocal laser endomicroscopy (pCLE) enables real-time optical biopsy. Little is known about pCLE imaging deep inside the small bowel, therefore the aim of this study was to determine its usefulness. PATIENTS AND METHODS: Between April 2014 and January 2016, we performed 38 pCLE examinations during double-balloon enteroscopy with intravenous fluorescein in 37 patients with: tumors (n = 10), vascular disorders (n = 6), inflammatory diseases and drug injuries (n = 13), other disorders (n = 4), and normal findings (n = 4). We measured the calibers of capillary and lymphatic vessels at 15 different sites and compared the calibers between pCLE images and histopathology. We also compared pCLE findings with pathologic diagnosis. RESULTS: The inner diameters of capillary vessels beneath the epithelium and in the middle of villi were 16.2 ± 3.0 µm and 14.5 ± 3.1 µm, respectively, in the pCLE images, but these were not consistent with formalin-fixed paraffin-embedded histology. In tumors, larger capillary vessels were observed in aggressive malignant lymphoma and metastasis, and a “soccer ball-like pattern” constituting homogenous dark cells packed with polygonal, narrower capillary vessels was characteristic in pCLE images of a malignant lymphoma follicle. Hereditary hemorrhagic telangiectasia and angiodysplasia contained anastomosis of capillary vessels of different calibers. In IgA vasculitis, segmental capillary strictures were observed. Intestinal lymphangiectasia with protein-losing enteropathy contained a reticular network of lymphatic vessels and dilated lymphatic ducts accompanied by numerous cell gaps. pCLE findings corresponded to pathologic diagnosis in 32 examinations (91 %). CONCLUSIONS: pCLE is useful for in vivo analysis of abnormalities of the capillary and lymphatic vessels and epithelium, and for diagnosis in various small-bowel diseases. © Georg Thieme Verlag KG 2017-07 2017-06-23 /pmc/articles/PMC5482742/ /pubmed/28670610 http://dx.doi.org/10.1055/s-0043-106184 Text en © Thieme Medical Publishers |
spellingShingle | Ohmiya, Naoki Horiguchi, Noriyuki Tahara, Tomomitsu Nagasaka, Mitsuo Nakagawa, Yoshihito Shibata, Tomoyuki Tsukamoto, Tetsuya Kuroda, Makoto In vivo characterization of abnormalities in small-bowel diseases using probe-based confocal laser endomicroscopy |
title |
In vivo characterization of abnormalities in small-bowel diseases using probe-based confocal laser endomicroscopy
|
title_full |
In vivo characterization of abnormalities in small-bowel diseases using probe-based confocal laser endomicroscopy
|
title_fullStr |
In vivo characterization of abnormalities in small-bowel diseases using probe-based confocal laser endomicroscopy
|
title_full_unstemmed |
In vivo characterization of abnormalities in small-bowel diseases using probe-based confocal laser endomicroscopy
|
title_short |
In vivo characterization of abnormalities in small-bowel diseases using probe-based confocal laser endomicroscopy
|
title_sort | in vivo characterization of abnormalities in small-bowel diseases using probe-based confocal laser endomicroscopy |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5482742/ https://www.ncbi.nlm.nih.gov/pubmed/28670610 http://dx.doi.org/10.1055/s-0043-106184 |
work_keys_str_mv | AT ohmiyanaoki invivocharacterizationofabnormalitiesinsmallboweldiseasesusingprobebasedconfocallaserendomicroscopy AT horiguchinoriyuki invivocharacterizationofabnormalitiesinsmallboweldiseasesusingprobebasedconfocallaserendomicroscopy AT taharatomomitsu invivocharacterizationofabnormalitiesinsmallboweldiseasesusingprobebasedconfocallaserendomicroscopy AT nagasakamitsuo invivocharacterizationofabnormalitiesinsmallboweldiseasesusingprobebasedconfocallaserendomicroscopy AT nakagawayoshihito invivocharacterizationofabnormalitiesinsmallboweldiseasesusingprobebasedconfocallaserendomicroscopy AT shibatatomoyuki invivocharacterizationofabnormalitiesinsmallboweldiseasesusingprobebasedconfocallaserendomicroscopy AT tsukamototetsuya invivocharacterizationofabnormalitiesinsmallboweldiseasesusingprobebasedconfocallaserendomicroscopy AT kurodamakoto invivocharacterizationofabnormalitiesinsmallboweldiseasesusingprobebasedconfocallaserendomicroscopy |