Using Fractional Intensities of Time-resolved Fluorescence to Sensitively Quantify NADH/NAD(+) with Genetically Encoded Fluorescent Biosensors

In this paper, we propose a novel and sensitive ratiometric analysis method that uses the fractional intensities of time-resolved fluorescence of genetically encoded fluorescent NADH/NAD(+) biosensors, Peredox, SoNar, and Frex. When the conformations of the biosensors change upon NADH/NAD(+) binding...

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Detalles Bibliográficos
Autores principales: Chang, Mengfang, Li, Lei, Hu, Hanyang, Hu, Qingxun, Wang, Aoxue, Cao, Xiaodan, Yu, Xiantong, Zhang, Sanjun, Zhao, Yuzheng, Chen, Jinquan, Yang, Yi, Xu, Jianhua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5482812/
https://www.ncbi.nlm.nih.gov/pubmed/28646144
http://dx.doi.org/10.1038/s41598-017-04051-7

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