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The C. elegans VAPB homolog VPR-1 is a permissive signal for gonad development

VAMP/synaptobrevin-associated proteins (VAPs) contain an N-terminal major sperm protein domain (MSPd) that is associated with amyotrophic lateral sclerosis. VAPs have an intracellular housekeeping function, as well as an extracellular signaling function mediated by the secreted MSPd. Here we show th...

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Autores principales: Cottee, Pauline A., Cole, Tim, Schultz, Jessica, Hoang, Hieu D., Vibbert, Jack, Han, Sung Min, Miller, Michael A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Ltd 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5482997/
https://www.ncbi.nlm.nih.gov/pubmed/28634273
http://dx.doi.org/10.1242/dev.152207
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author Cottee, Pauline A.
Cole, Tim
Schultz, Jessica
Hoang, Hieu D.
Vibbert, Jack
Han, Sung Min
Miller, Michael A.
author_facet Cottee, Pauline A.
Cole, Tim
Schultz, Jessica
Hoang, Hieu D.
Vibbert, Jack
Han, Sung Min
Miller, Michael A.
author_sort Cottee, Pauline A.
collection PubMed
description VAMP/synaptobrevin-associated proteins (VAPs) contain an N-terminal major sperm protein domain (MSPd) that is associated with amyotrophic lateral sclerosis. VAPs have an intracellular housekeeping function, as well as an extracellular signaling function mediated by the secreted MSPd. Here we show that the C. elegans VAP homolog VPR-1 is essential for gonad development. vpr-1 null mutants are maternal effect sterile due to arrested gonadogenesis following embryo hatching. Somatic gonadal precursor cells and germ cells fail to proliferate fully and complete their respective differentiation programs. Maternal or zygotic vpr-1 expression is sufficient to induce gonadogenesis and fertility. Genetic mosaic and cell type-specific expression studies indicate that vpr-1 activity is important in the nervous system, germ line and intestine. VPR-1 acts in parallel to Notch signaling, a key regulator of germline stem cell proliferation and differentiation. Neuronal vpr-1 expression is sufficient for gonadogenesis induction during a limited time period shortly after hatching. These results support the model that the secreted VPR-1 MSPd acts at least in part on gonadal sheath cell precursors in L1 to early L2 stage hermaphrodites to permit gonadogenesis.
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spelling pubmed-54829972017-07-11 The C. elegans VAPB homolog VPR-1 is a permissive signal for gonad development Cottee, Pauline A. Cole, Tim Schultz, Jessica Hoang, Hieu D. Vibbert, Jack Han, Sung Min Miller, Michael A. Development Research Article VAMP/synaptobrevin-associated proteins (VAPs) contain an N-terminal major sperm protein domain (MSPd) that is associated with amyotrophic lateral sclerosis. VAPs have an intracellular housekeeping function, as well as an extracellular signaling function mediated by the secreted MSPd. Here we show that the C. elegans VAP homolog VPR-1 is essential for gonad development. vpr-1 null mutants are maternal effect sterile due to arrested gonadogenesis following embryo hatching. Somatic gonadal precursor cells and germ cells fail to proliferate fully and complete their respective differentiation programs. Maternal or zygotic vpr-1 expression is sufficient to induce gonadogenesis and fertility. Genetic mosaic and cell type-specific expression studies indicate that vpr-1 activity is important in the nervous system, germ line and intestine. VPR-1 acts in parallel to Notch signaling, a key regulator of germline stem cell proliferation and differentiation. Neuronal vpr-1 expression is sufficient for gonadogenesis induction during a limited time period shortly after hatching. These results support the model that the secreted VPR-1 MSPd acts at least in part on gonadal sheath cell precursors in L1 to early L2 stage hermaphrodites to permit gonadogenesis. The Company of Biologists Ltd 2017-06-15 /pmc/articles/PMC5482997/ /pubmed/28634273 http://dx.doi.org/10.1242/dev.152207 Text en © 2017. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/3.0This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
spellingShingle Research Article
Cottee, Pauline A.
Cole, Tim
Schultz, Jessica
Hoang, Hieu D.
Vibbert, Jack
Han, Sung Min
Miller, Michael A.
The C. elegans VAPB homolog VPR-1 is a permissive signal for gonad development
title The C. elegans VAPB homolog VPR-1 is a permissive signal for gonad development
title_full The C. elegans VAPB homolog VPR-1 is a permissive signal for gonad development
title_fullStr The C. elegans VAPB homolog VPR-1 is a permissive signal for gonad development
title_full_unstemmed The C. elegans VAPB homolog VPR-1 is a permissive signal for gonad development
title_short The C. elegans VAPB homolog VPR-1 is a permissive signal for gonad development
title_sort c. elegans vapb homolog vpr-1 is a permissive signal for gonad development
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5482997/
https://www.ncbi.nlm.nih.gov/pubmed/28634273
http://dx.doi.org/10.1242/dev.152207
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