Cargando…
Fission yeast neddylation ligase Dcn1 facilitates cohesin cleavage and chromosome segregation at anaphase
Post-translational protein modification such as phosphorylation and ubiquitination are critical during mitosis to ensure proper timing and progression of chromosome segregation. It has been recently recognized that another type of protein modification – neddylation – may also regulate mitosis and ch...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Company of Biologists Ltd
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5483010/ https://www.ncbi.nlm.nih.gov/pubmed/28455357 http://dx.doi.org/10.1242/bio.021238 |
_version_ | 1783245675056594944 |
---|---|
author | Lin, Lan Chen, Li Tran, Phong T. |
author_facet | Lin, Lan Chen, Li Tran, Phong T. |
author_sort | Lin, Lan |
collection | PubMed |
description | Post-translational protein modification such as phosphorylation and ubiquitination are critical during mitosis to ensure proper timing and progression of chromosome segregation. It has been recently recognized that another type of protein modification – neddylation – may also regulate mitosis and chromosome segregation. The conserved protein DCN1 (defective cullin neddylation 1) has been shown, when knocked-down by RNAi, to result in multinucleated cells and/or blockage of cell proliferation. However, how DCN1 functions in mitosis and chromosome segregation is not known. We report here the fission yeast dcn1(+) and its role in mitosis and chromosome segregation. Dcn1-GFP localizes to the nucleus throughout the cell cycle. dcn1-deletion (dcn1Δ) leads to chromosome and kinetochore lagging at anaphase, resulting from delayed and attenuated cohesin cleavage and sister chromatids separation. These results put Dcn1 upstream of the anaphase promoting complex/cyclosome (APC/C) pathway. We propose a mechanism for Dcn1 function at mitosis. |
format | Online Article Text |
id | pubmed-5483010 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | The Company of Biologists Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-54830102017-06-28 Fission yeast neddylation ligase Dcn1 facilitates cohesin cleavage and chromosome segregation at anaphase Lin, Lan Chen, Li Tran, Phong T. Biol Open Research Article Post-translational protein modification such as phosphorylation and ubiquitination are critical during mitosis to ensure proper timing and progression of chromosome segregation. It has been recently recognized that another type of protein modification – neddylation – may also regulate mitosis and chromosome segregation. The conserved protein DCN1 (defective cullin neddylation 1) has been shown, when knocked-down by RNAi, to result in multinucleated cells and/or blockage of cell proliferation. However, how DCN1 functions in mitosis and chromosome segregation is not known. We report here the fission yeast dcn1(+) and its role in mitosis and chromosome segregation. Dcn1-GFP localizes to the nucleus throughout the cell cycle. dcn1-deletion (dcn1Δ) leads to chromosome and kinetochore lagging at anaphase, resulting from delayed and attenuated cohesin cleavage and sister chromatids separation. These results put Dcn1 upstream of the anaphase promoting complex/cyclosome (APC/C) pathway. We propose a mechanism for Dcn1 function at mitosis. The Company of Biologists Ltd 2017-04-28 /pmc/articles/PMC5483010/ /pubmed/28455357 http://dx.doi.org/10.1242/bio.021238 Text en © 2017. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/3.0This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed. |
spellingShingle | Research Article Lin, Lan Chen, Li Tran, Phong T. Fission yeast neddylation ligase Dcn1 facilitates cohesin cleavage and chromosome segregation at anaphase |
title | Fission yeast neddylation ligase Dcn1 facilitates cohesin cleavage and chromosome segregation at anaphase |
title_full | Fission yeast neddylation ligase Dcn1 facilitates cohesin cleavage and chromosome segregation at anaphase |
title_fullStr | Fission yeast neddylation ligase Dcn1 facilitates cohesin cleavage and chromosome segregation at anaphase |
title_full_unstemmed | Fission yeast neddylation ligase Dcn1 facilitates cohesin cleavage and chromosome segregation at anaphase |
title_short | Fission yeast neddylation ligase Dcn1 facilitates cohesin cleavage and chromosome segregation at anaphase |
title_sort | fission yeast neddylation ligase dcn1 facilitates cohesin cleavage and chromosome segregation at anaphase |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5483010/ https://www.ncbi.nlm.nih.gov/pubmed/28455357 http://dx.doi.org/10.1242/bio.021238 |
work_keys_str_mv | AT linlan fissionyeastneddylationligasedcn1facilitatescohesincleavageandchromosomesegregationatanaphase AT chenli fissionyeastneddylationligasedcn1facilitatescohesincleavageandchromosomesegregationatanaphase AT tranphongt fissionyeastneddylationligasedcn1facilitatescohesincleavageandchromosomesegregationatanaphase |