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Fission yeast neddylation ligase Dcn1 facilitates cohesin cleavage and chromosome segregation at anaphase

Post-translational protein modification such as phosphorylation and ubiquitination are critical during mitosis to ensure proper timing and progression of chromosome segregation. It has been recently recognized that another type of protein modification – neddylation – may also regulate mitosis and ch...

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Autores principales: Lin, Lan, Chen, Li, Tran, Phong T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Company of Biologists Ltd 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5483010/
https://www.ncbi.nlm.nih.gov/pubmed/28455357
http://dx.doi.org/10.1242/bio.021238
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author Lin, Lan
Chen, Li
Tran, Phong T.
author_facet Lin, Lan
Chen, Li
Tran, Phong T.
author_sort Lin, Lan
collection PubMed
description Post-translational protein modification such as phosphorylation and ubiquitination are critical during mitosis to ensure proper timing and progression of chromosome segregation. It has been recently recognized that another type of protein modification – neddylation – may also regulate mitosis and chromosome segregation. The conserved protein DCN1 (defective cullin neddylation 1) has been shown, when knocked-down by RNAi, to result in multinucleated cells and/or blockage of cell proliferation. However, how DCN1 functions in mitosis and chromosome segregation is not known. We report here the fission yeast dcn1(+) and its role in mitosis and chromosome segregation. Dcn1-GFP localizes to the nucleus throughout the cell cycle. dcn1-deletion (dcn1Δ) leads to chromosome and kinetochore lagging at anaphase, resulting from delayed and attenuated cohesin cleavage and sister chromatids separation. These results put Dcn1 upstream of the anaphase promoting complex/cyclosome (APC/C) pathway. We propose a mechanism for Dcn1 function at mitosis.
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spelling pubmed-54830102017-06-28 Fission yeast neddylation ligase Dcn1 facilitates cohesin cleavage and chromosome segregation at anaphase Lin, Lan Chen, Li Tran, Phong T. Biol Open Research Article Post-translational protein modification such as phosphorylation and ubiquitination are critical during mitosis to ensure proper timing and progression of chromosome segregation. It has been recently recognized that another type of protein modification – neddylation – may also regulate mitosis and chromosome segregation. The conserved protein DCN1 (defective cullin neddylation 1) has been shown, when knocked-down by RNAi, to result in multinucleated cells and/or blockage of cell proliferation. However, how DCN1 functions in mitosis and chromosome segregation is not known. We report here the fission yeast dcn1(+) and its role in mitosis and chromosome segregation. Dcn1-GFP localizes to the nucleus throughout the cell cycle. dcn1-deletion (dcn1Δ) leads to chromosome and kinetochore lagging at anaphase, resulting from delayed and attenuated cohesin cleavage and sister chromatids separation. These results put Dcn1 upstream of the anaphase promoting complex/cyclosome (APC/C) pathway. We propose a mechanism for Dcn1 function at mitosis. The Company of Biologists Ltd 2017-04-28 /pmc/articles/PMC5483010/ /pubmed/28455357 http://dx.doi.org/10.1242/bio.021238 Text en © 2017. Published by The Company of Biologists Ltd http://creativecommons.org/licenses/by/3.0This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
spellingShingle Research Article
Lin, Lan
Chen, Li
Tran, Phong T.
Fission yeast neddylation ligase Dcn1 facilitates cohesin cleavage and chromosome segregation at anaphase
title Fission yeast neddylation ligase Dcn1 facilitates cohesin cleavage and chromosome segregation at anaphase
title_full Fission yeast neddylation ligase Dcn1 facilitates cohesin cleavage and chromosome segregation at anaphase
title_fullStr Fission yeast neddylation ligase Dcn1 facilitates cohesin cleavage and chromosome segregation at anaphase
title_full_unstemmed Fission yeast neddylation ligase Dcn1 facilitates cohesin cleavage and chromosome segregation at anaphase
title_short Fission yeast neddylation ligase Dcn1 facilitates cohesin cleavage and chromosome segregation at anaphase
title_sort fission yeast neddylation ligase dcn1 facilitates cohesin cleavage and chromosome segregation at anaphase
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5483010/
https://www.ncbi.nlm.nih.gov/pubmed/28455357
http://dx.doi.org/10.1242/bio.021238
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