Cargando…

Iron homeostasis in neuronal cells: a role for IREG1

BACKGROUND: Iron is necessary for neuronal function but in excess generates neurodegeneration. Although most of the components of the iron homeostasis machinery have been described in neurons, little is known about the particulars of their iron homeostasis. In this work we characterized the response...

Descripción completa

Detalles Bibliográficos
Autores principales: Aguirre, Pabla, Mena, Natalia, Tapia, Victoria, Arredondo, Miguel, Núñez, Marco T
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC548319/
https://www.ncbi.nlm.nih.gov/pubmed/15667655
http://dx.doi.org/10.1186/1471-2202-6-3
_version_ 1782122342870155264
author Aguirre, Pabla
Mena, Natalia
Tapia, Victoria
Arredondo, Miguel
Núñez, Marco T
author_facet Aguirre, Pabla
Mena, Natalia
Tapia, Victoria
Arredondo, Miguel
Núñez, Marco T
author_sort Aguirre, Pabla
collection PubMed
description BACKGROUND: Iron is necessary for neuronal function but in excess generates neurodegeneration. Although most of the components of the iron homeostasis machinery have been described in neurons, little is known about the particulars of their iron homeostasis. In this work we characterized the response of SH-SY5Y neuroblastoma cells and hippocampal neurons to a model of progressive iron accumulation. RESULTS: We found that iron accumulation killed a large proportion of cells, but a sub-population became resistant to iron. The surviving cells evoked an adaptative response consisting of increased synthesis of the iron-storage protein ferritin and the iron export transporter IREG1, and decreased synthesis of the iron import transporter DMT1. Increased expression of IREG1 was further substantiated by immunocytochemistry and iron efflux experiments. IREG1 expression directly correlated with iron content in SH-SY5Y and hippocampal cells. Similarly, a high correlation was found between IREG1 expression and the rate of iron efflux from SH-SY5Y cells. CONCLUSIONS: Neuronal survival of iron accumulation associates with increased expression of the efflux transporter IREG1. Thus, the capacity of neurons to express IREG1 may be one of the clues to iron accumulation survival.
format Text
id pubmed-548319
institution National Center for Biotechnology Information
language English
publishDate 2005
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-5483192005-02-09 Iron homeostasis in neuronal cells: a role for IREG1 Aguirre, Pabla Mena, Natalia Tapia, Victoria Arredondo, Miguel Núñez, Marco T BMC Neurosci Research Article BACKGROUND: Iron is necessary for neuronal function but in excess generates neurodegeneration. Although most of the components of the iron homeostasis machinery have been described in neurons, little is known about the particulars of their iron homeostasis. In this work we characterized the response of SH-SY5Y neuroblastoma cells and hippocampal neurons to a model of progressive iron accumulation. RESULTS: We found that iron accumulation killed a large proportion of cells, but a sub-population became resistant to iron. The surviving cells evoked an adaptative response consisting of increased synthesis of the iron-storage protein ferritin and the iron export transporter IREG1, and decreased synthesis of the iron import transporter DMT1. Increased expression of IREG1 was further substantiated by immunocytochemistry and iron efflux experiments. IREG1 expression directly correlated with iron content in SH-SY5Y and hippocampal cells. Similarly, a high correlation was found between IREG1 expression and the rate of iron efflux from SH-SY5Y cells. CONCLUSIONS: Neuronal survival of iron accumulation associates with increased expression of the efflux transporter IREG1. Thus, the capacity of neurons to express IREG1 may be one of the clues to iron accumulation survival. BioMed Central 2005-01-24 /pmc/articles/PMC548319/ /pubmed/15667655 http://dx.doi.org/10.1186/1471-2202-6-3 Text en Copyright © 2005 Aguirre et al; licensee BioMed Central Ltd.
spellingShingle Research Article
Aguirre, Pabla
Mena, Natalia
Tapia, Victoria
Arredondo, Miguel
Núñez, Marco T
Iron homeostasis in neuronal cells: a role for IREG1
title Iron homeostasis in neuronal cells: a role for IREG1
title_full Iron homeostasis in neuronal cells: a role for IREG1
title_fullStr Iron homeostasis in neuronal cells: a role for IREG1
title_full_unstemmed Iron homeostasis in neuronal cells: a role for IREG1
title_short Iron homeostasis in neuronal cells: a role for IREG1
title_sort iron homeostasis in neuronal cells: a role for ireg1
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC548319/
https://www.ncbi.nlm.nih.gov/pubmed/15667655
http://dx.doi.org/10.1186/1471-2202-6-3
work_keys_str_mv AT aguirrepabla ironhomeostasisinneuronalcellsaroleforireg1
AT menanatalia ironhomeostasisinneuronalcellsaroleforireg1
AT tapiavictoria ironhomeostasisinneuronalcellsaroleforireg1
AT arredondomiguel ironhomeostasisinneuronalcellsaroleforireg1
AT nunezmarcot ironhomeostasisinneuronalcellsaroleforireg1