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Iron homeostasis in neuronal cells: a role for IREG1
BACKGROUND: Iron is necessary for neuronal function but in excess generates neurodegeneration. Although most of the components of the iron homeostasis machinery have been described in neurons, little is known about the particulars of their iron homeostasis. In this work we characterized the response...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2005
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC548319/ https://www.ncbi.nlm.nih.gov/pubmed/15667655 http://dx.doi.org/10.1186/1471-2202-6-3 |
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author | Aguirre, Pabla Mena, Natalia Tapia, Victoria Arredondo, Miguel Núñez, Marco T |
author_facet | Aguirre, Pabla Mena, Natalia Tapia, Victoria Arredondo, Miguel Núñez, Marco T |
author_sort | Aguirre, Pabla |
collection | PubMed |
description | BACKGROUND: Iron is necessary for neuronal function but in excess generates neurodegeneration. Although most of the components of the iron homeostasis machinery have been described in neurons, little is known about the particulars of their iron homeostasis. In this work we characterized the response of SH-SY5Y neuroblastoma cells and hippocampal neurons to a model of progressive iron accumulation. RESULTS: We found that iron accumulation killed a large proportion of cells, but a sub-population became resistant to iron. The surviving cells evoked an adaptative response consisting of increased synthesis of the iron-storage protein ferritin and the iron export transporter IREG1, and decreased synthesis of the iron import transporter DMT1. Increased expression of IREG1 was further substantiated by immunocytochemistry and iron efflux experiments. IREG1 expression directly correlated with iron content in SH-SY5Y and hippocampal cells. Similarly, a high correlation was found between IREG1 expression and the rate of iron efflux from SH-SY5Y cells. CONCLUSIONS: Neuronal survival of iron accumulation associates with increased expression of the efflux transporter IREG1. Thus, the capacity of neurons to express IREG1 may be one of the clues to iron accumulation survival. |
format | Text |
id | pubmed-548319 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2005 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-5483192005-02-09 Iron homeostasis in neuronal cells: a role for IREG1 Aguirre, Pabla Mena, Natalia Tapia, Victoria Arredondo, Miguel Núñez, Marco T BMC Neurosci Research Article BACKGROUND: Iron is necessary for neuronal function but in excess generates neurodegeneration. Although most of the components of the iron homeostasis machinery have been described in neurons, little is known about the particulars of their iron homeostasis. In this work we characterized the response of SH-SY5Y neuroblastoma cells and hippocampal neurons to a model of progressive iron accumulation. RESULTS: We found that iron accumulation killed a large proportion of cells, but a sub-population became resistant to iron. The surviving cells evoked an adaptative response consisting of increased synthesis of the iron-storage protein ferritin and the iron export transporter IREG1, and decreased synthesis of the iron import transporter DMT1. Increased expression of IREG1 was further substantiated by immunocytochemistry and iron efflux experiments. IREG1 expression directly correlated with iron content in SH-SY5Y and hippocampal cells. Similarly, a high correlation was found between IREG1 expression and the rate of iron efflux from SH-SY5Y cells. CONCLUSIONS: Neuronal survival of iron accumulation associates with increased expression of the efflux transporter IREG1. Thus, the capacity of neurons to express IREG1 may be one of the clues to iron accumulation survival. BioMed Central 2005-01-24 /pmc/articles/PMC548319/ /pubmed/15667655 http://dx.doi.org/10.1186/1471-2202-6-3 Text en Copyright © 2005 Aguirre et al; licensee BioMed Central Ltd. |
spellingShingle | Research Article Aguirre, Pabla Mena, Natalia Tapia, Victoria Arredondo, Miguel Núñez, Marco T Iron homeostasis in neuronal cells: a role for IREG1 |
title | Iron homeostasis in neuronal cells: a role for IREG1 |
title_full | Iron homeostasis in neuronal cells: a role for IREG1 |
title_fullStr | Iron homeostasis in neuronal cells: a role for IREG1 |
title_full_unstemmed | Iron homeostasis in neuronal cells: a role for IREG1 |
title_short | Iron homeostasis in neuronal cells: a role for IREG1 |
title_sort | iron homeostasis in neuronal cells: a role for ireg1 |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC548319/ https://www.ncbi.nlm.nih.gov/pubmed/15667655 http://dx.doi.org/10.1186/1471-2202-6-3 |
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