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An isolated cellulolytic Escherichia coli from bovine rumen produces ethanol and hydrogen from corn straw
BACKGROUND: Lignocellulosic biomass is the most abundant resource on earth. Lignocellulose is mainly composed of cellulose, hemicelluloses, and lignin. The special construction of three kinds of constituents led to the prevention of effective degradation. The goal of this work was to investigate the...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5483281/ https://www.ncbi.nlm.nih.gov/pubmed/28652866 http://dx.doi.org/10.1186/s13068-017-0852-7 |
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author | Pang, Jian Liu, Zhan-Ying Hao, Min Zhang, Yong-Feng Qi, Qing-Sheng |
author_facet | Pang, Jian Liu, Zhan-Ying Hao, Min Zhang, Yong-Feng Qi, Qing-Sheng |
author_sort | Pang, Jian |
collection | PubMed |
description | BACKGROUND: Lignocellulosic biomass is the most abundant resource on earth. Lignocellulose is mainly composed of cellulose, hemicelluloses, and lignin. The special construction of three kinds of constituents led to the prevention of effective degradation. The goal of this work was to investigate the great potentials of bovine rumen for novel cellulolytic bacterial isolation, which may be used for chemicals and biofuel production from lignocellulose. RESULTS: A cellulolytic strain, ZH-4, was isolated from Inner Mongolia bovine rumen. This strain was identified as Escherichia coli by morphological, physiological, and biochemical characteristics and 16S rDNA gene sequencing. The extracellular enzyme activity analysis showed that this strain produces extracellular cellulases with an exoglucanase activity of 9.13 IU, an endoglucanase activity of 5.31 IU, and a β-glucosidase activity of 7.27 IU at the pH 6.8. This strain was found to produce 0.36 g/L ethanol and 4.71 mL/g hydrogen from corn straw with cellulose degradation ratio of 14.30% and hemicellulose degradation ratio of 11.39%. CONCLUSIONS: It is the first time that a cellulolytic E. coli was isolated and characterized form the bovine rumen. This provided a great opportunity for researchers to investigate the evolution mechanisms of the microorganisms in the rumen and provided great chance to produce biofuels and chemicals directly from engineered E. coli using consolidated bioprocess. |
format | Online Article Text |
id | pubmed-5483281 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-54832812017-06-26 An isolated cellulolytic Escherichia coli from bovine rumen produces ethanol and hydrogen from corn straw Pang, Jian Liu, Zhan-Ying Hao, Min Zhang, Yong-Feng Qi, Qing-Sheng Biotechnol Biofuels Research BACKGROUND: Lignocellulosic biomass is the most abundant resource on earth. Lignocellulose is mainly composed of cellulose, hemicelluloses, and lignin. The special construction of three kinds of constituents led to the prevention of effective degradation. The goal of this work was to investigate the great potentials of bovine rumen for novel cellulolytic bacterial isolation, which may be used for chemicals and biofuel production from lignocellulose. RESULTS: A cellulolytic strain, ZH-4, was isolated from Inner Mongolia bovine rumen. This strain was identified as Escherichia coli by morphological, physiological, and biochemical characteristics and 16S rDNA gene sequencing. The extracellular enzyme activity analysis showed that this strain produces extracellular cellulases with an exoglucanase activity of 9.13 IU, an endoglucanase activity of 5.31 IU, and a β-glucosidase activity of 7.27 IU at the pH 6.8. This strain was found to produce 0.36 g/L ethanol and 4.71 mL/g hydrogen from corn straw with cellulose degradation ratio of 14.30% and hemicellulose degradation ratio of 11.39%. CONCLUSIONS: It is the first time that a cellulolytic E. coli was isolated and characterized form the bovine rumen. This provided a great opportunity for researchers to investigate the evolution mechanisms of the microorganisms in the rumen and provided great chance to produce biofuels and chemicals directly from engineered E. coli using consolidated bioprocess. BioMed Central 2017-06-24 /pmc/articles/PMC5483281/ /pubmed/28652866 http://dx.doi.org/10.1186/s13068-017-0852-7 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Pang, Jian Liu, Zhan-Ying Hao, Min Zhang, Yong-Feng Qi, Qing-Sheng An isolated cellulolytic Escherichia coli from bovine rumen produces ethanol and hydrogen from corn straw |
title | An isolated cellulolytic Escherichia coli from bovine rumen produces ethanol and hydrogen from corn straw |
title_full | An isolated cellulolytic Escherichia coli from bovine rumen produces ethanol and hydrogen from corn straw |
title_fullStr | An isolated cellulolytic Escherichia coli from bovine rumen produces ethanol and hydrogen from corn straw |
title_full_unstemmed | An isolated cellulolytic Escherichia coli from bovine rumen produces ethanol and hydrogen from corn straw |
title_short | An isolated cellulolytic Escherichia coli from bovine rumen produces ethanol and hydrogen from corn straw |
title_sort | isolated cellulolytic escherichia coli from bovine rumen produces ethanol and hydrogen from corn straw |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5483281/ https://www.ncbi.nlm.nih.gov/pubmed/28652866 http://dx.doi.org/10.1186/s13068-017-0852-7 |
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