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An isolated cellulolytic Escherichia coli from bovine rumen produces ethanol and hydrogen from corn straw

BACKGROUND: Lignocellulosic biomass is the most abundant resource on earth. Lignocellulose is mainly composed of cellulose, hemicelluloses, and lignin. The special construction of three kinds of constituents led to the prevention of effective degradation. The goal of this work was to investigate the...

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Autores principales: Pang, Jian, Liu, Zhan-Ying, Hao, Min, Zhang, Yong-Feng, Qi, Qing-Sheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5483281/
https://www.ncbi.nlm.nih.gov/pubmed/28652866
http://dx.doi.org/10.1186/s13068-017-0852-7
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author Pang, Jian
Liu, Zhan-Ying
Hao, Min
Zhang, Yong-Feng
Qi, Qing-Sheng
author_facet Pang, Jian
Liu, Zhan-Ying
Hao, Min
Zhang, Yong-Feng
Qi, Qing-Sheng
author_sort Pang, Jian
collection PubMed
description BACKGROUND: Lignocellulosic biomass is the most abundant resource on earth. Lignocellulose is mainly composed of cellulose, hemicelluloses, and lignin. The special construction of three kinds of constituents led to the prevention of effective degradation. The goal of this work was to investigate the great potentials of bovine rumen for novel cellulolytic bacterial isolation, which may be used for chemicals and biofuel production from lignocellulose. RESULTS: A cellulolytic strain, ZH-4, was isolated from Inner Mongolia bovine rumen. This strain was identified as Escherichia coli by morphological, physiological, and biochemical characteristics and 16S rDNA gene sequencing. The extracellular enzyme activity analysis showed that this strain produces extracellular cellulases with an exoglucanase activity of 9.13 IU, an endoglucanase activity of 5.31 IU, and a β-glucosidase activity of 7.27 IU at the pH 6.8. This strain was found to produce 0.36 g/L ethanol and 4.71 mL/g hydrogen from corn straw with cellulose degradation ratio of 14.30% and hemicellulose degradation ratio of 11.39%. CONCLUSIONS: It is the first time that a cellulolytic E. coli was isolated and characterized form the bovine rumen. This provided a great opportunity for researchers to investigate the evolution mechanisms of the microorganisms in the rumen and provided great chance to produce biofuels and chemicals directly from engineered E. coli using consolidated bioprocess.
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spelling pubmed-54832812017-06-26 An isolated cellulolytic Escherichia coli from bovine rumen produces ethanol and hydrogen from corn straw Pang, Jian Liu, Zhan-Ying Hao, Min Zhang, Yong-Feng Qi, Qing-Sheng Biotechnol Biofuels Research BACKGROUND: Lignocellulosic biomass is the most abundant resource on earth. Lignocellulose is mainly composed of cellulose, hemicelluloses, and lignin. The special construction of three kinds of constituents led to the prevention of effective degradation. The goal of this work was to investigate the great potentials of bovine rumen for novel cellulolytic bacterial isolation, which may be used for chemicals and biofuel production from lignocellulose. RESULTS: A cellulolytic strain, ZH-4, was isolated from Inner Mongolia bovine rumen. This strain was identified as Escherichia coli by morphological, physiological, and biochemical characteristics and 16S rDNA gene sequencing. The extracellular enzyme activity analysis showed that this strain produces extracellular cellulases with an exoglucanase activity of 9.13 IU, an endoglucanase activity of 5.31 IU, and a β-glucosidase activity of 7.27 IU at the pH 6.8. This strain was found to produce 0.36 g/L ethanol and 4.71 mL/g hydrogen from corn straw with cellulose degradation ratio of 14.30% and hemicellulose degradation ratio of 11.39%. CONCLUSIONS: It is the first time that a cellulolytic E. coli was isolated and characterized form the bovine rumen. This provided a great opportunity for researchers to investigate the evolution mechanisms of the microorganisms in the rumen and provided great chance to produce biofuels and chemicals directly from engineered E. coli using consolidated bioprocess. BioMed Central 2017-06-24 /pmc/articles/PMC5483281/ /pubmed/28652866 http://dx.doi.org/10.1186/s13068-017-0852-7 Text en © The Author(s) 2017 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Pang, Jian
Liu, Zhan-Ying
Hao, Min
Zhang, Yong-Feng
Qi, Qing-Sheng
An isolated cellulolytic Escherichia coli from bovine rumen produces ethanol and hydrogen from corn straw
title An isolated cellulolytic Escherichia coli from bovine rumen produces ethanol and hydrogen from corn straw
title_full An isolated cellulolytic Escherichia coli from bovine rumen produces ethanol and hydrogen from corn straw
title_fullStr An isolated cellulolytic Escherichia coli from bovine rumen produces ethanol and hydrogen from corn straw
title_full_unstemmed An isolated cellulolytic Escherichia coli from bovine rumen produces ethanol and hydrogen from corn straw
title_short An isolated cellulolytic Escherichia coli from bovine rumen produces ethanol and hydrogen from corn straw
title_sort isolated cellulolytic escherichia coli from bovine rumen produces ethanol and hydrogen from corn straw
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5483281/
https://www.ncbi.nlm.nih.gov/pubmed/28652866
http://dx.doi.org/10.1186/s13068-017-0852-7
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