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Bridging Plant and Human Radiation Response and DNA Repair through an In Silico Approach

The mechanisms of response to radiation exposure are conserved in plants and animals. The DNA damage response (DDR) pathways are the predominant molecular pathways activated upon exposure to radiation, both in plants and animals. The conserved features of DDR in plants and animals might facilitate i...

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Autores principales: Nikitaki, Zacharenia, Pavlopoulou, Athanasia, Holá, Marcela, Donà, Mattia, Michalopoulos, Ioannis, Balestrazzi, Alma, Angelis, Karel J., Georgakilas, Alexandros G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5483884/
https://www.ncbi.nlm.nih.gov/pubmed/28587301
http://dx.doi.org/10.3390/cancers9060065
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author Nikitaki, Zacharenia
Pavlopoulou, Athanasia
Holá, Marcela
Donà, Mattia
Michalopoulos, Ioannis
Balestrazzi, Alma
Angelis, Karel J.
Georgakilas, Alexandros G.
author_facet Nikitaki, Zacharenia
Pavlopoulou, Athanasia
Holá, Marcela
Donà, Mattia
Michalopoulos, Ioannis
Balestrazzi, Alma
Angelis, Karel J.
Georgakilas, Alexandros G.
author_sort Nikitaki, Zacharenia
collection PubMed
description The mechanisms of response to radiation exposure are conserved in plants and animals. The DNA damage response (DDR) pathways are the predominant molecular pathways activated upon exposure to radiation, both in plants and animals. The conserved features of DDR in plants and animals might facilitate interdisciplinary studies that cross traditional boundaries between animal and plant biology in order to expand the collection of biomarkers currently used for radiation exposure monitoring (REM) in environmental and biomedical settings. Genes implicated in trans-kingdom conserved DDR networks often triggered by ionizing radiation (IR) and UV light are deposited into biological databases. In this study, we have applied an innovative approach utilizing data pertinent to plant and human genes from publicly available databases towards the design of a ‘plant radiation biodosimeter’, that is, a plant and DDR gene-based platform that could serve as a REM reliable biomarker for assessing environmental radiation exposure and associated risk. From our analysis, in addition to REM biomarkers, a significant number of genes, both in human and Arabidopsis thaliana, not yet characterized as DDR, are suggested as possible DNA repair players. Last but not least, we provide an example on the applicability of an Arabidopsis thaliana—based plant system monitoring the role of cancer-related DNA repair genes BRCA1, BARD1 and PARP1 in processing DNA lesions.
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spelling pubmed-54838842017-06-28 Bridging Plant and Human Radiation Response and DNA Repair through an In Silico Approach Nikitaki, Zacharenia Pavlopoulou, Athanasia Holá, Marcela Donà, Mattia Michalopoulos, Ioannis Balestrazzi, Alma Angelis, Karel J. Georgakilas, Alexandros G. Cancers (Basel) Article The mechanisms of response to radiation exposure are conserved in plants and animals. The DNA damage response (DDR) pathways are the predominant molecular pathways activated upon exposure to radiation, both in plants and animals. The conserved features of DDR in plants and animals might facilitate interdisciplinary studies that cross traditional boundaries between animal and plant biology in order to expand the collection of biomarkers currently used for radiation exposure monitoring (REM) in environmental and biomedical settings. Genes implicated in trans-kingdom conserved DDR networks often triggered by ionizing radiation (IR) and UV light are deposited into biological databases. In this study, we have applied an innovative approach utilizing data pertinent to plant and human genes from publicly available databases towards the design of a ‘plant radiation biodosimeter’, that is, a plant and DDR gene-based platform that could serve as a REM reliable biomarker for assessing environmental radiation exposure and associated risk. From our analysis, in addition to REM biomarkers, a significant number of genes, both in human and Arabidopsis thaliana, not yet characterized as DDR, are suggested as possible DNA repair players. Last but not least, we provide an example on the applicability of an Arabidopsis thaliana—based plant system monitoring the role of cancer-related DNA repair genes BRCA1, BARD1 and PARP1 in processing DNA lesions. MDPI 2017-06-06 /pmc/articles/PMC5483884/ /pubmed/28587301 http://dx.doi.org/10.3390/cancers9060065 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nikitaki, Zacharenia
Pavlopoulou, Athanasia
Holá, Marcela
Donà, Mattia
Michalopoulos, Ioannis
Balestrazzi, Alma
Angelis, Karel J.
Georgakilas, Alexandros G.
Bridging Plant and Human Radiation Response and DNA Repair through an In Silico Approach
title Bridging Plant and Human Radiation Response and DNA Repair through an In Silico Approach
title_full Bridging Plant and Human Radiation Response and DNA Repair through an In Silico Approach
title_fullStr Bridging Plant and Human Radiation Response and DNA Repair through an In Silico Approach
title_full_unstemmed Bridging Plant and Human Radiation Response and DNA Repair through an In Silico Approach
title_short Bridging Plant and Human Radiation Response and DNA Repair through an In Silico Approach
title_sort bridging plant and human radiation response and dna repair through an in silico approach
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5483884/
https://www.ncbi.nlm.nih.gov/pubmed/28587301
http://dx.doi.org/10.3390/cancers9060065
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