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Collagen from the Marine Sponges Axinella cannabina and Suberites carnosus: Isolation and Morphological, Biochemical, and Biophysical Characterization
In search of alternative and safer sources of collagen for biomedical applications, the marine demosponges Axinella cannabina and Suberites carnosus, collected from the Aegean and the Ionian Seas, respectively, were comparatively studied for their insoluble collagen, intercellular collagen, and spon...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5484102/ https://www.ncbi.nlm.nih.gov/pubmed/28555046 http://dx.doi.org/10.3390/md15060152 |
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author | Tziveleka, Leto-Aikaterini Ioannou, Efstathia Tsiourvas, Dimitris Berillis, Panagiotis Foufa, Evangelia Roussis, Vassilios |
author_facet | Tziveleka, Leto-Aikaterini Ioannou, Efstathia Tsiourvas, Dimitris Berillis, Panagiotis Foufa, Evangelia Roussis, Vassilios |
author_sort | Tziveleka, Leto-Aikaterini |
collection | PubMed |
description | In search of alternative and safer sources of collagen for biomedical applications, the marine demosponges Axinella cannabina and Suberites carnosus, collected from the Aegean and the Ionian Seas, respectively, were comparatively studied for their insoluble collagen, intercellular collagen, and spongin-like collagen content. The isolated collagenous materials were morphologically, physicochemically, and biophysically characterized. Using scanning electron microscopy and transmission electron microscopy the fibrous morphology of the isolated collagens was confirmed, whereas the amino acid analysis, in conjunction with infrared spectroscopy studies, verified the characteristic for the collagen amino acid profile and its secondary structure. Furthermore, the isoelectric point and thermal behavior were determined by titration and differential scanning calorimetry, in combination with circular dichroism spectroscopic studies, respectively. |
format | Online Article Text |
id | pubmed-5484102 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-54841022017-06-29 Collagen from the Marine Sponges Axinella cannabina and Suberites carnosus: Isolation and Morphological, Biochemical, and Biophysical Characterization Tziveleka, Leto-Aikaterini Ioannou, Efstathia Tsiourvas, Dimitris Berillis, Panagiotis Foufa, Evangelia Roussis, Vassilios Mar Drugs Article In search of alternative and safer sources of collagen for biomedical applications, the marine demosponges Axinella cannabina and Suberites carnosus, collected from the Aegean and the Ionian Seas, respectively, were comparatively studied for their insoluble collagen, intercellular collagen, and spongin-like collagen content. The isolated collagenous materials were morphologically, physicochemically, and biophysically characterized. Using scanning electron microscopy and transmission electron microscopy the fibrous morphology of the isolated collagens was confirmed, whereas the amino acid analysis, in conjunction with infrared spectroscopy studies, verified the characteristic for the collagen amino acid profile and its secondary structure. Furthermore, the isoelectric point and thermal behavior were determined by titration and differential scanning calorimetry, in combination with circular dichroism spectroscopic studies, respectively. MDPI 2017-05-29 /pmc/articles/PMC5484102/ /pubmed/28555046 http://dx.doi.org/10.3390/md15060152 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Tziveleka, Leto-Aikaterini Ioannou, Efstathia Tsiourvas, Dimitris Berillis, Panagiotis Foufa, Evangelia Roussis, Vassilios Collagen from the Marine Sponges Axinella cannabina and Suberites carnosus: Isolation and Morphological, Biochemical, and Biophysical Characterization |
title | Collagen from the Marine Sponges Axinella cannabina and Suberites carnosus: Isolation and Morphological, Biochemical, and Biophysical Characterization |
title_full | Collagen from the Marine Sponges Axinella cannabina and Suberites carnosus: Isolation and Morphological, Biochemical, and Biophysical Characterization |
title_fullStr | Collagen from the Marine Sponges Axinella cannabina and Suberites carnosus: Isolation and Morphological, Biochemical, and Biophysical Characterization |
title_full_unstemmed | Collagen from the Marine Sponges Axinella cannabina and Suberites carnosus: Isolation and Morphological, Biochemical, and Biophysical Characterization |
title_short | Collagen from the Marine Sponges Axinella cannabina and Suberites carnosus: Isolation and Morphological, Biochemical, and Biophysical Characterization |
title_sort | collagen from the marine sponges axinella cannabina and suberites carnosus: isolation and morphological, biochemical, and biophysical characterization |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5484102/ https://www.ncbi.nlm.nih.gov/pubmed/28555046 http://dx.doi.org/10.3390/md15060152 |
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