The Missing Piece in Biosynthesis of Amphidinols: First Evidence of Glycolate as a Starter Unit in New Polyketides from Amphidinium carterae

Two new members of the amphidinol family, amphidinol A (1) and its 7-sulfate derivative amphidinol B (2), were isolated from a strain of Amphidinium carterae of Lake Fusaro, near Naples (Italy), and chemically identified by spectroscopic and spectrometric methods. Amphidinol A showed antifungal acti...

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Detalles Bibliográficos
Autores principales: Cutignano, Adele, Nuzzo, Genoveffa, Sardo, Angela, Fontana, Angelo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5484107/
https://www.ncbi.nlm.nih.gov/pubmed/28561749
http://dx.doi.org/10.3390/md15060157
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author Cutignano, Adele
Nuzzo, Genoveffa
Sardo, Angela
Fontana, Angelo
author_facet Cutignano, Adele
Nuzzo, Genoveffa
Sardo, Angela
Fontana, Angelo
author_sort Cutignano, Adele
collection PubMed
description Two new members of the amphidinol family, amphidinol A (1) and its 7-sulfate derivative amphidinol B (2), were isolated from a strain of Amphidinium carterae of Lake Fusaro, near Naples (Italy), and chemically identified by spectroscopic and spectrometric methods. Amphidinol A showed antifungal activity against Candida albicans (MIC = 19 µg/mL). Biosynthetic experiments with stable isotope-labelled acetate allowed defining the elongation process in 1. For the first time the use of glycolate as a starter unit in the polyketide biosynthesis of amphidinol metabolites was unambiguously demonstrated.
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spelling pubmed-54841072017-06-29 The Missing Piece in Biosynthesis of Amphidinols: First Evidence of Glycolate as a Starter Unit in New Polyketides from Amphidinium carterae Cutignano, Adele Nuzzo, Genoveffa Sardo, Angela Fontana, Angelo Mar Drugs Article Two new members of the amphidinol family, amphidinol A (1) and its 7-sulfate derivative amphidinol B (2), were isolated from a strain of Amphidinium carterae of Lake Fusaro, near Naples (Italy), and chemically identified by spectroscopic and spectrometric methods. Amphidinol A showed antifungal activity against Candida albicans (MIC = 19 µg/mL). Biosynthetic experiments with stable isotope-labelled acetate allowed defining the elongation process in 1. For the first time the use of glycolate as a starter unit in the polyketide biosynthesis of amphidinol metabolites was unambiguously demonstrated. MDPI 2017-05-31 /pmc/articles/PMC5484107/ /pubmed/28561749 http://dx.doi.org/10.3390/md15060157 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cutignano, Adele
Nuzzo, Genoveffa
Sardo, Angela
Fontana, Angelo
The Missing Piece in Biosynthesis of Amphidinols: First Evidence of Glycolate as a Starter Unit in New Polyketides from Amphidinium carterae
title The Missing Piece in Biosynthesis of Amphidinols: First Evidence of Glycolate as a Starter Unit in New Polyketides from Amphidinium carterae
title_full The Missing Piece in Biosynthesis of Amphidinols: First Evidence of Glycolate as a Starter Unit in New Polyketides from Amphidinium carterae
title_fullStr The Missing Piece in Biosynthesis of Amphidinols: First Evidence of Glycolate as a Starter Unit in New Polyketides from Amphidinium carterae
title_full_unstemmed The Missing Piece in Biosynthesis of Amphidinols: First Evidence of Glycolate as a Starter Unit in New Polyketides from Amphidinium carterae
title_short The Missing Piece in Biosynthesis of Amphidinols: First Evidence of Glycolate as a Starter Unit in New Polyketides from Amphidinium carterae
title_sort missing piece in biosynthesis of amphidinols: first evidence of glycolate as a starter unit in new polyketides from amphidinium carterae
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5484107/
https://www.ncbi.nlm.nih.gov/pubmed/28561749
http://dx.doi.org/10.3390/md15060157
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