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Chondroitin Sulfate-Rich Extract of Skate Cartilage Attenuates Lipopolysaccharide-Induced Liver Damage in Mice

The protective effects of a chondroitin sulfate-rich extract (CSE) from skate cartilage against lipopolysaccharide (LPS)-induced hepatic damage were investigated, and its mechanism of action was compared with that of chondroitin sulfate (CS) from shark cartilage. ICR mice were orally administrated 2...

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Autores principales: Song, Yeong Ok, Kim, Mijeong, Woo, Minji, Baek, Jang-Mi, Kang, Keon-Hee, Kim, Sang-Ho, Roh, Seong-Soo, Park, Chan Hum, Jeong, Kap-Seop, Noh, Jeong-Sook
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5484128/
https://www.ncbi.nlm.nih.gov/pubmed/28617322
http://dx.doi.org/10.3390/md15060178
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author Song, Yeong Ok
Kim, Mijeong
Woo, Minji
Baek, Jang-Mi
Kang, Keon-Hee
Kim, Sang-Ho
Roh, Seong-Soo
Park, Chan Hum
Jeong, Kap-Seop
Noh, Jeong-Sook
author_facet Song, Yeong Ok
Kim, Mijeong
Woo, Minji
Baek, Jang-Mi
Kang, Keon-Hee
Kim, Sang-Ho
Roh, Seong-Soo
Park, Chan Hum
Jeong, Kap-Seop
Noh, Jeong-Sook
author_sort Song, Yeong Ok
collection PubMed
description The protective effects of a chondroitin sulfate-rich extract (CSE) from skate cartilage against lipopolysaccharide (LPS)-induced hepatic damage were investigated, and its mechanism of action was compared with that of chondroitin sulfate (CS) from shark cartilage. ICR mice were orally administrated 200 mg/kg body weight (BW) of CS or 400 mg/kg BW of CSE for 3 consecutive days, followed by a one-time intraperitoneal injection of LPS (20 mg/kg BW). The experimental groups were vehicle treatment without LPS injection (NC group), vehicle treatment with LPS injection (LPS group), CS pretreatment with LPS injection (CS group), and CSE pretreatment with LPS injection (CSE group). Hepatic antioxidant enzyme expression levels in the CS and CSE groups were increased relative to those in the LPS group. In LPS-insulted hepatic tissue, inflammatory factors were augmented relative to those in the NC group, but were significantly suppressed by pretreatment with CS or CSE. Moreover, CS and CSE alleviated the LPS-induced apoptotic factors and mitogen-activated protein kinase (MAPK). In addition, CS and CSE effectively decreased the serum lipid concentrations and downregulated hepatic sterol regulatory element-binding proteins expression. In conclusion, the skate CSE could protect against LPS-induced hepatic dyslipidemia, oxidative stress, inflammation, and apoptosis, probably through the regulation of MAPK signaling.
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spelling pubmed-54841282017-06-29 Chondroitin Sulfate-Rich Extract of Skate Cartilage Attenuates Lipopolysaccharide-Induced Liver Damage in Mice Song, Yeong Ok Kim, Mijeong Woo, Minji Baek, Jang-Mi Kang, Keon-Hee Kim, Sang-Ho Roh, Seong-Soo Park, Chan Hum Jeong, Kap-Seop Noh, Jeong-Sook Mar Drugs Article The protective effects of a chondroitin sulfate-rich extract (CSE) from skate cartilage against lipopolysaccharide (LPS)-induced hepatic damage were investigated, and its mechanism of action was compared with that of chondroitin sulfate (CS) from shark cartilage. ICR mice were orally administrated 200 mg/kg body weight (BW) of CS or 400 mg/kg BW of CSE for 3 consecutive days, followed by a one-time intraperitoneal injection of LPS (20 mg/kg BW). The experimental groups were vehicle treatment without LPS injection (NC group), vehicle treatment with LPS injection (LPS group), CS pretreatment with LPS injection (CS group), and CSE pretreatment with LPS injection (CSE group). Hepatic antioxidant enzyme expression levels in the CS and CSE groups were increased relative to those in the LPS group. In LPS-insulted hepatic tissue, inflammatory factors were augmented relative to those in the NC group, but were significantly suppressed by pretreatment with CS or CSE. Moreover, CS and CSE alleviated the LPS-induced apoptotic factors and mitogen-activated protein kinase (MAPK). In addition, CS and CSE effectively decreased the serum lipid concentrations and downregulated hepatic sterol regulatory element-binding proteins expression. In conclusion, the skate CSE could protect against LPS-induced hepatic dyslipidemia, oxidative stress, inflammation, and apoptosis, probably through the regulation of MAPK signaling. MDPI 2017-06-15 /pmc/articles/PMC5484128/ /pubmed/28617322 http://dx.doi.org/10.3390/md15060178 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Song, Yeong Ok
Kim, Mijeong
Woo, Minji
Baek, Jang-Mi
Kang, Keon-Hee
Kim, Sang-Ho
Roh, Seong-Soo
Park, Chan Hum
Jeong, Kap-Seop
Noh, Jeong-Sook
Chondroitin Sulfate-Rich Extract of Skate Cartilage Attenuates Lipopolysaccharide-Induced Liver Damage in Mice
title Chondroitin Sulfate-Rich Extract of Skate Cartilage Attenuates Lipopolysaccharide-Induced Liver Damage in Mice
title_full Chondroitin Sulfate-Rich Extract of Skate Cartilage Attenuates Lipopolysaccharide-Induced Liver Damage in Mice
title_fullStr Chondroitin Sulfate-Rich Extract of Skate Cartilage Attenuates Lipopolysaccharide-Induced Liver Damage in Mice
title_full_unstemmed Chondroitin Sulfate-Rich Extract of Skate Cartilage Attenuates Lipopolysaccharide-Induced Liver Damage in Mice
title_short Chondroitin Sulfate-Rich Extract of Skate Cartilage Attenuates Lipopolysaccharide-Induced Liver Damage in Mice
title_sort chondroitin sulfate-rich extract of skate cartilage attenuates lipopolysaccharide-induced liver damage in mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5484128/
https://www.ncbi.nlm.nih.gov/pubmed/28617322
http://dx.doi.org/10.3390/md15060178
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