Cargando…
Novel loop-mediated isothermal amplification (LAMP) assay with a universal QProbe can detect SNPs determining races in plant pathogenic fungi
Tomato wilt pathogen Fusarium oxysporum f. sp. lycopersici (Fol) is grouped into three races based on their pathogenicity to different host cultivars. Rapid detection and discrimination of Fol races in field soils is important to prevent tomato wilt disease. Although five types of point mutations in...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2017
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5484703/ https://www.ncbi.nlm.nih.gov/pubmed/28652587 http://dx.doi.org/10.1038/s41598-017-04084-y |
_version_ | 1783245928489025536 |
---|---|
author | Ayukawa, Yu Hanyuda, Saeri Fujita, Naoko Komatsu, Ken Arie, Tsutomu |
author_facet | Ayukawa, Yu Hanyuda, Saeri Fujita, Naoko Komatsu, Ken Arie, Tsutomu |
author_sort | Ayukawa, Yu |
collection | PubMed |
description | Tomato wilt pathogen Fusarium oxysporum f. sp. lycopersici (Fol) is grouped into three races based on their pathogenicity to different host cultivars. Rapid detection and discrimination of Fol races in field soils is important to prevent tomato wilt disease. Although five types of point mutations in secreted in xylem 3 (SIX3) gene, which are characteristic of race 3, have been reported as a molecular marker for the race, detection of these point mutations is laborious. The aim of this study is to develop a rapid and accurate method for the detection of point mutations in SIX3 of Fol. Loop-mediated isothermal amplification (LAMP) of SIX3 gene with the universal QProbe as well as two joint DNAs followed by annealing curve analysis allowed us to specifically detect Fol and discriminate race 3 among other races in about one hour. Our developed method is applicable for detection of races of other plant pathogenic fungi as well as their pesticide-resistant mutants that arise through point mutations in a particular gene. |
format | Online Article Text |
id | pubmed-5484703 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-54847032017-06-30 Novel loop-mediated isothermal amplification (LAMP) assay with a universal QProbe can detect SNPs determining races in plant pathogenic fungi Ayukawa, Yu Hanyuda, Saeri Fujita, Naoko Komatsu, Ken Arie, Tsutomu Sci Rep Article Tomato wilt pathogen Fusarium oxysporum f. sp. lycopersici (Fol) is grouped into three races based on their pathogenicity to different host cultivars. Rapid detection and discrimination of Fol races in field soils is important to prevent tomato wilt disease. Although five types of point mutations in secreted in xylem 3 (SIX3) gene, which are characteristic of race 3, have been reported as a molecular marker for the race, detection of these point mutations is laborious. The aim of this study is to develop a rapid and accurate method for the detection of point mutations in SIX3 of Fol. Loop-mediated isothermal amplification (LAMP) of SIX3 gene with the universal QProbe as well as two joint DNAs followed by annealing curve analysis allowed us to specifically detect Fol and discriminate race 3 among other races in about one hour. Our developed method is applicable for detection of races of other plant pathogenic fungi as well as their pesticide-resistant mutants that arise through point mutations in a particular gene. Nature Publishing Group UK 2017-06-26 /pmc/articles/PMC5484703/ /pubmed/28652587 http://dx.doi.org/10.1038/s41598-017-04084-y Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Ayukawa, Yu Hanyuda, Saeri Fujita, Naoko Komatsu, Ken Arie, Tsutomu Novel loop-mediated isothermal amplification (LAMP) assay with a universal QProbe can detect SNPs determining races in plant pathogenic fungi |
title | Novel loop-mediated isothermal amplification (LAMP) assay with a universal QProbe can detect SNPs determining races in plant pathogenic fungi |
title_full | Novel loop-mediated isothermal amplification (LAMP) assay with a universal QProbe can detect SNPs determining races in plant pathogenic fungi |
title_fullStr | Novel loop-mediated isothermal amplification (LAMP) assay with a universal QProbe can detect SNPs determining races in plant pathogenic fungi |
title_full_unstemmed | Novel loop-mediated isothermal amplification (LAMP) assay with a universal QProbe can detect SNPs determining races in plant pathogenic fungi |
title_short | Novel loop-mediated isothermal amplification (LAMP) assay with a universal QProbe can detect SNPs determining races in plant pathogenic fungi |
title_sort | novel loop-mediated isothermal amplification (lamp) assay with a universal qprobe can detect snps determining races in plant pathogenic fungi |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5484703/ https://www.ncbi.nlm.nih.gov/pubmed/28652587 http://dx.doi.org/10.1038/s41598-017-04084-y |
work_keys_str_mv | AT ayukawayu novelloopmediatedisothermalamplificationlampassaywithauniversalqprobecandetectsnpsdeterminingracesinplantpathogenicfungi AT hanyudasaeri novelloopmediatedisothermalamplificationlampassaywithauniversalqprobecandetectsnpsdeterminingracesinplantpathogenicfungi AT fujitanaoko novelloopmediatedisothermalamplificationlampassaywithauniversalqprobecandetectsnpsdeterminingracesinplantpathogenicfungi AT komatsuken novelloopmediatedisothermalamplificationlampassaywithauniversalqprobecandetectsnpsdeterminingracesinplantpathogenicfungi AT arietsutomu novelloopmediatedisothermalamplificationlampassaywithauniversalqprobecandetectsnpsdeterminingracesinplantpathogenicfungi |