Cargando…

Novel loop-mediated isothermal amplification (LAMP) assay with a universal QProbe can detect SNPs determining races in plant pathogenic fungi

Tomato wilt pathogen Fusarium oxysporum f. sp. lycopersici (Fol) is grouped into three races based on their pathogenicity to different host cultivars. Rapid detection and discrimination of Fol races in field soils is important to prevent tomato wilt disease. Although five types of point mutations in...

Descripción completa

Detalles Bibliográficos
Autores principales: Ayukawa, Yu, Hanyuda, Saeri, Fujita, Naoko, Komatsu, Ken, Arie, Tsutomu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5484703/
https://www.ncbi.nlm.nih.gov/pubmed/28652587
http://dx.doi.org/10.1038/s41598-017-04084-y
_version_ 1783245928489025536
author Ayukawa, Yu
Hanyuda, Saeri
Fujita, Naoko
Komatsu, Ken
Arie, Tsutomu
author_facet Ayukawa, Yu
Hanyuda, Saeri
Fujita, Naoko
Komatsu, Ken
Arie, Tsutomu
author_sort Ayukawa, Yu
collection PubMed
description Tomato wilt pathogen Fusarium oxysporum f. sp. lycopersici (Fol) is grouped into three races based on their pathogenicity to different host cultivars. Rapid detection and discrimination of Fol races in field soils is important to prevent tomato wilt disease. Although five types of point mutations in secreted in xylem 3 (SIX3) gene, which are characteristic of race 3, have been reported as a molecular marker for the race, detection of these point mutations is laborious. The aim of this study is to develop a rapid and accurate method for the detection of point mutations in SIX3 of Fol. Loop-mediated isothermal amplification (LAMP) of SIX3 gene with the universal QProbe as well as two joint DNAs followed by annealing curve analysis allowed us to specifically detect Fol and discriminate race 3 among other races in about one hour. Our developed method is applicable for detection of races of other plant pathogenic fungi as well as their pesticide-resistant mutants that arise through point mutations in a particular gene.
format Online
Article
Text
id pubmed-5484703
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-54847032017-06-30 Novel loop-mediated isothermal amplification (LAMP) assay with a universal QProbe can detect SNPs determining races in plant pathogenic fungi Ayukawa, Yu Hanyuda, Saeri Fujita, Naoko Komatsu, Ken Arie, Tsutomu Sci Rep Article Tomato wilt pathogen Fusarium oxysporum f. sp. lycopersici (Fol) is grouped into three races based on their pathogenicity to different host cultivars. Rapid detection and discrimination of Fol races in field soils is important to prevent tomato wilt disease. Although five types of point mutations in secreted in xylem 3 (SIX3) gene, which are characteristic of race 3, have been reported as a molecular marker for the race, detection of these point mutations is laborious. The aim of this study is to develop a rapid and accurate method for the detection of point mutations in SIX3 of Fol. Loop-mediated isothermal amplification (LAMP) of SIX3 gene with the universal QProbe as well as two joint DNAs followed by annealing curve analysis allowed us to specifically detect Fol and discriminate race 3 among other races in about one hour. Our developed method is applicable for detection of races of other plant pathogenic fungi as well as their pesticide-resistant mutants that arise through point mutations in a particular gene. Nature Publishing Group UK 2017-06-26 /pmc/articles/PMC5484703/ /pubmed/28652587 http://dx.doi.org/10.1038/s41598-017-04084-y Text en © The Author(s) 2017 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Ayukawa, Yu
Hanyuda, Saeri
Fujita, Naoko
Komatsu, Ken
Arie, Tsutomu
Novel loop-mediated isothermal amplification (LAMP) assay with a universal QProbe can detect SNPs determining races in plant pathogenic fungi
title Novel loop-mediated isothermal amplification (LAMP) assay with a universal QProbe can detect SNPs determining races in plant pathogenic fungi
title_full Novel loop-mediated isothermal amplification (LAMP) assay with a universal QProbe can detect SNPs determining races in plant pathogenic fungi
title_fullStr Novel loop-mediated isothermal amplification (LAMP) assay with a universal QProbe can detect SNPs determining races in plant pathogenic fungi
title_full_unstemmed Novel loop-mediated isothermal amplification (LAMP) assay with a universal QProbe can detect SNPs determining races in plant pathogenic fungi
title_short Novel loop-mediated isothermal amplification (LAMP) assay with a universal QProbe can detect SNPs determining races in plant pathogenic fungi
title_sort novel loop-mediated isothermal amplification (lamp) assay with a universal qprobe can detect snps determining races in plant pathogenic fungi
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5484703/
https://www.ncbi.nlm.nih.gov/pubmed/28652587
http://dx.doi.org/10.1038/s41598-017-04084-y
work_keys_str_mv AT ayukawayu novelloopmediatedisothermalamplificationlampassaywithauniversalqprobecandetectsnpsdeterminingracesinplantpathogenicfungi
AT hanyudasaeri novelloopmediatedisothermalamplificationlampassaywithauniversalqprobecandetectsnpsdeterminingracesinplantpathogenicfungi
AT fujitanaoko novelloopmediatedisothermalamplificationlampassaywithauniversalqprobecandetectsnpsdeterminingracesinplantpathogenicfungi
AT komatsuken novelloopmediatedisothermalamplificationlampassaywithauniversalqprobecandetectsnpsdeterminingracesinplantpathogenicfungi
AT arietsutomu novelloopmediatedisothermalamplificationlampassaywithauniversalqprobecandetectsnpsdeterminingracesinplantpathogenicfungi