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Purification and Characterization of an ATPase GsiA from Salmonella enterica

The coding sequence of Salmonella enterica gsiA was cloned and expressed in E. coli. The protein was purified and ATPase activity was characterized by NADH oxidation method. GsiA exhibited optimum activity at 30°C and at pH 8 in Tris/HCl buffer. GsiA protein was stable at 20°C. 66% and 44% activity...

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Detalles Bibliográficos
Autores principales: Wang, Zhongshan, Zhang, Meng, Shi, Xiaodong, Xiang, Quanju
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5485302/
https://www.ncbi.nlm.nih.gov/pubmed/28691022
http://dx.doi.org/10.1155/2017/3076091

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