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Role of ARPC2 in Human Gastric Cancer

Gastric cancer continues to be the second most frequent cause of cancer deaths worldwide. However, the exact molecular mechanisms are still unclear. Further research to find potential targets for therapy is critical and urgent. In this study, we found that ARPC2 promoted cell proliferation and invas...

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Autores principales: Zhang, Jun, Liu, Yi, Yu, Chang-Jun, Dai, Fu, Xiong, Jie, Li, Hong-Jun, Wu, Zheng-Sheng, Ding, Rui, Wang, Hong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5485321/
https://www.ncbi.nlm.nih.gov/pubmed/28694563
http://dx.doi.org/10.1155/2017/5432818
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author Zhang, Jun
Liu, Yi
Yu, Chang-Jun
Dai, Fu
Xiong, Jie
Li, Hong-Jun
Wu, Zheng-Sheng
Ding, Rui
Wang, Hong
author_facet Zhang, Jun
Liu, Yi
Yu, Chang-Jun
Dai, Fu
Xiong, Jie
Li, Hong-Jun
Wu, Zheng-Sheng
Ding, Rui
Wang, Hong
author_sort Zhang, Jun
collection PubMed
description Gastric cancer continues to be the second most frequent cause of cancer deaths worldwide. However, the exact molecular mechanisms are still unclear. Further research to find potential targets for therapy is critical and urgent. In this study, we found that ARPC2 promoted cell proliferation and invasion in the human cancer cell line MKN-28 using a cell total number assay, MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide) assay, cell colony formation assay, migration assay, invasion assay, and wound healing assay. For downstream pathways, CTNND1, EZH2, BCL2L2, CDH2, VIM, and EGFR were upregulated by ARPC2, whereas PTEN, BAK, and CDH1 were downregulated by ARPC2. In a clinical study, we examined the expression of ARPC2 in 110 cases of normal human gastric tissues and 110 cases of human gastric cancer tissues. ARPC2 showed higher expression in gastric cancer tissues than in normal gastric tissues. In the association analysis of 110 gastric cancer tissues, ARPC2 showed significant associations with large tumor size, lymph node invasion, and high tumor stage. In addition, ARPC2-positive patients exhibited lower RFS and OS rates compared with ARPC2-negative patients. We thus identify that ARPC2 plays an aneretic role in human gastric cancer and provided a new target for gastric cancer therapy.
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spelling pubmed-54853212017-07-10 Role of ARPC2 in Human Gastric Cancer Zhang, Jun Liu, Yi Yu, Chang-Jun Dai, Fu Xiong, Jie Li, Hong-Jun Wu, Zheng-Sheng Ding, Rui Wang, Hong Mediators Inflamm Research Article Gastric cancer continues to be the second most frequent cause of cancer deaths worldwide. However, the exact molecular mechanisms are still unclear. Further research to find potential targets for therapy is critical and urgent. In this study, we found that ARPC2 promoted cell proliferation and invasion in the human cancer cell line MKN-28 using a cell total number assay, MTT (3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide) assay, cell colony formation assay, migration assay, invasion assay, and wound healing assay. For downstream pathways, CTNND1, EZH2, BCL2L2, CDH2, VIM, and EGFR were upregulated by ARPC2, whereas PTEN, BAK, and CDH1 were downregulated by ARPC2. In a clinical study, we examined the expression of ARPC2 in 110 cases of normal human gastric tissues and 110 cases of human gastric cancer tissues. ARPC2 showed higher expression in gastric cancer tissues than in normal gastric tissues. In the association analysis of 110 gastric cancer tissues, ARPC2 showed significant associations with large tumor size, lymph node invasion, and high tumor stage. In addition, ARPC2-positive patients exhibited lower RFS and OS rates compared with ARPC2-negative patients. We thus identify that ARPC2 plays an aneretic role in human gastric cancer and provided a new target for gastric cancer therapy. Hindawi 2017 2017-06-13 /pmc/articles/PMC5485321/ /pubmed/28694563 http://dx.doi.org/10.1155/2017/5432818 Text en Copyright © 2017 Jun Zhang et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zhang, Jun
Liu, Yi
Yu, Chang-Jun
Dai, Fu
Xiong, Jie
Li, Hong-Jun
Wu, Zheng-Sheng
Ding, Rui
Wang, Hong
Role of ARPC2 in Human Gastric Cancer
title Role of ARPC2 in Human Gastric Cancer
title_full Role of ARPC2 in Human Gastric Cancer
title_fullStr Role of ARPC2 in Human Gastric Cancer
title_full_unstemmed Role of ARPC2 in Human Gastric Cancer
title_short Role of ARPC2 in Human Gastric Cancer
title_sort role of arpc2 in human gastric cancer
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5485321/
https://www.ncbi.nlm.nih.gov/pubmed/28694563
http://dx.doi.org/10.1155/2017/5432818
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