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Identification of Highly Specific scFvs against Total Adiponectin for Diagnostic Purposes
Adiponectin is one of the most abundant adipokines secreted from adipose tissue. It acts as an endogenous insulin sensitizer and plasma concentrations are inversely correlated with obesity and metabolic syndrome. A decrease in plasma adiponectin levels normally indicates increased hormonal activity...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5485473/ https://www.ncbi.nlm.nih.gov/pubmed/28445413 http://dx.doi.org/10.3390/biology6020026 |
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author | Wilton, Peter Steidel, Michael Krczal, Gabriele Hermanns, Iris Pfützner, Andreas Konnerth, Alisa Boonrod, Kajohn |
author_facet | Wilton, Peter Steidel, Michael Krczal, Gabriele Hermanns, Iris Pfützner, Andreas Konnerth, Alisa Boonrod, Kajohn |
author_sort | Wilton, Peter |
collection | PubMed |
description | Adiponectin is one of the most abundant adipokines secreted from adipose tissue. It acts as an endogenous insulin sensitizer and plasma concentrations are inversely correlated with obesity and metabolic syndrome. A decrease in plasma adiponectin levels normally indicates increased hormonal activity of the visceral lipid tissue, which is associated with decreased insulin sensitivity. It may therefore be considered a valuable biomarker for elucidating the underlying deteriorations resulting in type 2 diabetes and macrovascular disease. Here we present the use of phage display technology to identify highly specific antibody fragments (scFvs) against adiponectin. The selected scFvs showed highly specific binding to globular and native adiponectin in ELISA tests. By using our phage display technology, we were able to obtain monoclonal antibodies with specific high affinity binding to the target protein in an effective and easy to upscale manner. The selected scFvs against adiponectin can be used for developing immunoassays suitable for use in metabolic syndrome diagnosis and monitoring. |
format | Online Article Text |
id | pubmed-5485473 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-54854732017-06-29 Identification of Highly Specific scFvs against Total Adiponectin for Diagnostic Purposes Wilton, Peter Steidel, Michael Krczal, Gabriele Hermanns, Iris Pfützner, Andreas Konnerth, Alisa Boonrod, Kajohn Biology (Basel) Article Adiponectin is one of the most abundant adipokines secreted from adipose tissue. It acts as an endogenous insulin sensitizer and plasma concentrations are inversely correlated with obesity and metabolic syndrome. A decrease in plasma adiponectin levels normally indicates increased hormonal activity of the visceral lipid tissue, which is associated with decreased insulin sensitivity. It may therefore be considered a valuable biomarker for elucidating the underlying deteriorations resulting in type 2 diabetes and macrovascular disease. Here we present the use of phage display technology to identify highly specific antibody fragments (scFvs) against adiponectin. The selected scFvs showed highly specific binding to globular and native adiponectin in ELISA tests. By using our phage display technology, we were able to obtain monoclonal antibodies with specific high affinity binding to the target protein in an effective and easy to upscale manner. The selected scFvs against adiponectin can be used for developing immunoassays suitable for use in metabolic syndrome diagnosis and monitoring. MDPI 2017-04-26 /pmc/articles/PMC5485473/ /pubmed/28445413 http://dx.doi.org/10.3390/biology6020026 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wilton, Peter Steidel, Michael Krczal, Gabriele Hermanns, Iris Pfützner, Andreas Konnerth, Alisa Boonrod, Kajohn Identification of Highly Specific scFvs against Total Adiponectin for Diagnostic Purposes |
title | Identification of Highly Specific scFvs against Total Adiponectin for Diagnostic Purposes |
title_full | Identification of Highly Specific scFvs against Total Adiponectin for Diagnostic Purposes |
title_fullStr | Identification of Highly Specific scFvs against Total Adiponectin for Diagnostic Purposes |
title_full_unstemmed | Identification of Highly Specific scFvs against Total Adiponectin for Diagnostic Purposes |
title_short | Identification of Highly Specific scFvs against Total Adiponectin for Diagnostic Purposes |
title_sort | identification of highly specific scfvs against total adiponectin for diagnostic purposes |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5485473/ https://www.ncbi.nlm.nih.gov/pubmed/28445413 http://dx.doi.org/10.3390/biology6020026 |
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