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Fine Mapping and Transcriptome Analysis Reveal Candidate Genes Associated with Hybrid Lethality in Cabbage (Brassica Oleracea)

Hybrid lethality is a deleterious phenotype that is vital to species evolution. We previously reported hybrid lethality in cabbage (Brassica oleracea) and performed preliminary mapping of related genes. In the present study, the fine mapping of hybrid lethal genes revealed that BoHL1 was located on...

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Autores principales: Xiao, Zhiliang, Hu, Yang, Zhang, Xiaoli, Xue, Yuqian, Fang, Zhiyuan, Yang, Limei, Zhang, Yangyong, Liu, Yumei, Li, Zhansheng, Liu, Xing, Liu, Zezhou, Lv, Honghao, Zhuang, Mu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5485511/
https://www.ncbi.nlm.nih.gov/pubmed/28587228
http://dx.doi.org/10.3390/genes8060147
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author Xiao, Zhiliang
Hu, Yang
Zhang, Xiaoli
Xue, Yuqian
Fang, Zhiyuan
Yang, Limei
Zhang, Yangyong
Liu, Yumei
Li, Zhansheng
Liu, Xing
Liu, Zezhou
Lv, Honghao
Zhuang, Mu
author_facet Xiao, Zhiliang
Hu, Yang
Zhang, Xiaoli
Xue, Yuqian
Fang, Zhiyuan
Yang, Limei
Zhang, Yangyong
Liu, Yumei
Li, Zhansheng
Liu, Xing
Liu, Zezhou
Lv, Honghao
Zhuang, Mu
author_sort Xiao, Zhiliang
collection PubMed
description Hybrid lethality is a deleterious phenotype that is vital to species evolution. We previously reported hybrid lethality in cabbage (Brassica oleracea) and performed preliminary mapping of related genes. In the present study, the fine mapping of hybrid lethal genes revealed that BoHL1 was located on chromosome C1 between BoHLTO124 and BoHLTO130, with an interval of 101 kb. BoHL2 was confirmed to be between insertion-deletion (InDels) markers HL234 and HL235 on C4, with a marker interval of 70 kb. Twenty-eight and nine annotated genes were found within the two intervals of BoHL1 and BoHL2, respectively. We also applied RNA-Seq to analyze hybrid lethality in cabbage. In the region of BoHL1, seven differentially expressed genes (DEGs) and five resistance (R)-related genes (two in common, i.e., Bo1g153320 and Bo1g153380) were found, whereas in the region of BoHL2, two DEGs and four R-related genes (two in common, i.e., Bo4g173780 and Bo4g173810) were found. Along with studies in which R genes were frequently involved in hybrid lethality in other plants, these interesting R-DEGs may be good candidates associated with hybrid lethality. We also used SNP/InDel analyses and quantitative real-time PCR to confirm the results. This work provides new insight into the mechanisms of hybrid lethality in cabbage.
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spelling pubmed-54855112017-06-29 Fine Mapping and Transcriptome Analysis Reveal Candidate Genes Associated with Hybrid Lethality in Cabbage (Brassica Oleracea) Xiao, Zhiliang Hu, Yang Zhang, Xiaoli Xue, Yuqian Fang, Zhiyuan Yang, Limei Zhang, Yangyong Liu, Yumei Li, Zhansheng Liu, Xing Liu, Zezhou Lv, Honghao Zhuang, Mu Genes (Basel) Article Hybrid lethality is a deleterious phenotype that is vital to species evolution. We previously reported hybrid lethality in cabbage (Brassica oleracea) and performed preliminary mapping of related genes. In the present study, the fine mapping of hybrid lethal genes revealed that BoHL1 was located on chromosome C1 between BoHLTO124 and BoHLTO130, with an interval of 101 kb. BoHL2 was confirmed to be between insertion-deletion (InDels) markers HL234 and HL235 on C4, with a marker interval of 70 kb. Twenty-eight and nine annotated genes were found within the two intervals of BoHL1 and BoHL2, respectively. We also applied RNA-Seq to analyze hybrid lethality in cabbage. In the region of BoHL1, seven differentially expressed genes (DEGs) and five resistance (R)-related genes (two in common, i.e., Bo1g153320 and Bo1g153380) were found, whereas in the region of BoHL2, two DEGs and four R-related genes (two in common, i.e., Bo4g173780 and Bo4g173810) were found. Along with studies in which R genes were frequently involved in hybrid lethality in other plants, these interesting R-DEGs may be good candidates associated with hybrid lethality. We also used SNP/InDel analyses and quantitative real-time PCR to confirm the results. This work provides new insight into the mechanisms of hybrid lethality in cabbage. MDPI 2017-06-05 /pmc/articles/PMC5485511/ /pubmed/28587228 http://dx.doi.org/10.3390/genes8060147 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Xiao, Zhiliang
Hu, Yang
Zhang, Xiaoli
Xue, Yuqian
Fang, Zhiyuan
Yang, Limei
Zhang, Yangyong
Liu, Yumei
Li, Zhansheng
Liu, Xing
Liu, Zezhou
Lv, Honghao
Zhuang, Mu
Fine Mapping and Transcriptome Analysis Reveal Candidate Genes Associated with Hybrid Lethality in Cabbage (Brassica Oleracea)
title Fine Mapping and Transcriptome Analysis Reveal Candidate Genes Associated with Hybrid Lethality in Cabbage (Brassica Oleracea)
title_full Fine Mapping and Transcriptome Analysis Reveal Candidate Genes Associated with Hybrid Lethality in Cabbage (Brassica Oleracea)
title_fullStr Fine Mapping and Transcriptome Analysis Reveal Candidate Genes Associated with Hybrid Lethality in Cabbage (Brassica Oleracea)
title_full_unstemmed Fine Mapping and Transcriptome Analysis Reveal Candidate Genes Associated with Hybrid Lethality in Cabbage (Brassica Oleracea)
title_short Fine Mapping and Transcriptome Analysis Reveal Candidate Genes Associated with Hybrid Lethality in Cabbage (Brassica Oleracea)
title_sort fine mapping and transcriptome analysis reveal candidate genes associated with hybrid lethality in cabbage (brassica oleracea)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5485511/
https://www.ncbi.nlm.nih.gov/pubmed/28587228
http://dx.doi.org/10.3390/genes8060147
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