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Reticulate Evolution of the Rock Lizards: Meiotic Chromosome Dynamics and Spermatogenesis in Diploid and Triploid Males of the Genus Darevskia

Knowing whether triploid hybrids resulting from natural hybridization of parthenogenetic and bisexual species are fertile is crucial for understanding the mechanisms of reticulate evolution in rock lizards. Here, using males of the bisexual diploid rock lizard species Darevskia raddei nairensis and...

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Autores principales: Spangenberg, Victor, Arakelyan, Marine, Galoyan, Eduard, Matveevsky, Sergey, Petrosyan, Ruzanna, Bogdanov, Yuri, Danielyan, Felix, Kolomiets, Oxana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5485513/
https://www.ncbi.nlm.nih.gov/pubmed/28538689
http://dx.doi.org/10.3390/genes8060149
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author Spangenberg, Victor
Arakelyan, Marine
Galoyan, Eduard
Matveevsky, Sergey
Petrosyan, Ruzanna
Bogdanov, Yuri
Danielyan, Felix
Kolomiets, Oxana
author_facet Spangenberg, Victor
Arakelyan, Marine
Galoyan, Eduard
Matveevsky, Sergey
Petrosyan, Ruzanna
Bogdanov, Yuri
Danielyan, Felix
Kolomiets, Oxana
author_sort Spangenberg, Victor
collection PubMed
description Knowing whether triploid hybrids resulting from natural hybridization of parthenogenetic and bisexual species are fertile is crucial for understanding the mechanisms of reticulate evolution in rock lizards. Here, using males of the bisexual diploid rock lizard species Darevskia raddei nairensis and Darevskia valentini and a triploid hybrid male Darevskia unisexualis × Darevskia valentini, we performed karyotyping and comparative immunocytochemistry of chromosome synapsis and investigated the distribution of RAD51 and MLH1 foci in spread spermatocyte nuclei in meiotic prophase I. Three chromosome sets were found to occur in cell nuclei in the D. unisexualis × D. valentini hybrid, two originating from a parthenogenetic D. unisexualis female and one from the D. valentini male. Despite this distorted chromosome synapsis and incomplete double-strand breaks repair in meiotic prophase I, the number of mismatch repair foci in the triploid hybrid was enough to pass through both meiotic divisions. The defects in synapsis and repair did not arrest meiosis or spermatogenesis. Numerous abnormal mature spermatids were observed in the testes of the studied hybrid.
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spelling pubmed-54855132017-06-29 Reticulate Evolution of the Rock Lizards: Meiotic Chromosome Dynamics and Spermatogenesis in Diploid and Triploid Males of the Genus Darevskia Spangenberg, Victor Arakelyan, Marine Galoyan, Eduard Matveevsky, Sergey Petrosyan, Ruzanna Bogdanov, Yuri Danielyan, Felix Kolomiets, Oxana Genes (Basel) Article Knowing whether triploid hybrids resulting from natural hybridization of parthenogenetic and bisexual species are fertile is crucial for understanding the mechanisms of reticulate evolution in rock lizards. Here, using males of the bisexual diploid rock lizard species Darevskia raddei nairensis and Darevskia valentini and a triploid hybrid male Darevskia unisexualis × Darevskia valentini, we performed karyotyping and comparative immunocytochemistry of chromosome synapsis and investigated the distribution of RAD51 and MLH1 foci in spread spermatocyte nuclei in meiotic prophase I. Three chromosome sets were found to occur in cell nuclei in the D. unisexualis × D. valentini hybrid, two originating from a parthenogenetic D. unisexualis female and one from the D. valentini male. Despite this distorted chromosome synapsis and incomplete double-strand breaks repair in meiotic prophase I, the number of mismatch repair foci in the triploid hybrid was enough to pass through both meiotic divisions. The defects in synapsis and repair did not arrest meiosis or spermatogenesis. Numerous abnormal mature spermatids were observed in the testes of the studied hybrid. MDPI 2017-05-24 /pmc/articles/PMC5485513/ /pubmed/28538689 http://dx.doi.org/10.3390/genes8060149 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Spangenberg, Victor
Arakelyan, Marine
Galoyan, Eduard
Matveevsky, Sergey
Petrosyan, Ruzanna
Bogdanov, Yuri
Danielyan, Felix
Kolomiets, Oxana
Reticulate Evolution of the Rock Lizards: Meiotic Chromosome Dynamics and Spermatogenesis in Diploid and Triploid Males of the Genus Darevskia
title Reticulate Evolution of the Rock Lizards: Meiotic Chromosome Dynamics and Spermatogenesis in Diploid and Triploid Males of the Genus Darevskia
title_full Reticulate Evolution of the Rock Lizards: Meiotic Chromosome Dynamics and Spermatogenesis in Diploid and Triploid Males of the Genus Darevskia
title_fullStr Reticulate Evolution of the Rock Lizards: Meiotic Chromosome Dynamics and Spermatogenesis in Diploid and Triploid Males of the Genus Darevskia
title_full_unstemmed Reticulate Evolution of the Rock Lizards: Meiotic Chromosome Dynamics and Spermatogenesis in Diploid and Triploid Males of the Genus Darevskia
title_short Reticulate Evolution of the Rock Lizards: Meiotic Chromosome Dynamics and Spermatogenesis in Diploid and Triploid Males of the Genus Darevskia
title_sort reticulate evolution of the rock lizards: meiotic chromosome dynamics and spermatogenesis in diploid and triploid males of the genus darevskia
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5485513/
https://www.ncbi.nlm.nih.gov/pubmed/28538689
http://dx.doi.org/10.3390/genes8060149
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