Mutagenesis of threonine to serine in the active site of Mycobacterium tuberculosis fructose-1,6-bisphosphatase (Class II) retains partial enzyme activity

The glpX gene encodes for the Class II fructose-1,6-bisphosphatase enzyme in Mycobacterium tuberculosis (Mt), an essential enzyme for pathogenesis. We have performed site directed mutagenesis to introduce two mutations at residue Thr84, T84A and T84S, to explore the binding affinity of the substrate...

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Detalles Bibliográficos
Autores principales: Bondoc, Jasper Marc G., Wolf, Nina M., Ndichuck, Michael, Abad-Zapatero, Celerino, Movahedzadeh, Farahnaz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2017
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5485559/
https://www.ncbi.nlm.nih.gov/pubmed/28702369
http://dx.doi.org/10.1016/j.btre.2017.06.004

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