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Dioscin Induces Gallbladder Cancer Apoptosis by Inhibiting ROS-Mediated PI3K/AKT Signalling

Gallbladder cancer (GBC), highly aggressive form of cancer with an extremely poor prognosis, is the most common malignancy of the biliary tract. In this study, we investigated the effects of dioscin (DSN) on human GBC and the potential mechanisms underlying these effects. The results showed that DSN...

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Autores principales: Song, Xiaoling, Wang, Zheng, Liang, Haibin, Zhang, Wenjie, Ye, Yuanyuan, Li, HuaiFeng, Hu, Yunping, Zhang, Yijian, Weng, Hao, Lu, Jianhua, Wang, Xuefeng, Li, Maolan, Liu, Yingbin, Gu, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5485633/
https://www.ncbi.nlm.nih.gov/pubmed/28656003
http://dx.doi.org/10.7150/ijbs.18732
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author Song, Xiaoling
Wang, Zheng
Liang, Haibin
Zhang, Wenjie
Ye, Yuanyuan
Li, HuaiFeng
Hu, Yunping
Zhang, Yijian
Weng, Hao
Lu, Jianhua
Wang, Xuefeng
Li, Maolan
Liu, Yingbin
Gu, Jun
author_facet Song, Xiaoling
Wang, Zheng
Liang, Haibin
Zhang, Wenjie
Ye, Yuanyuan
Li, HuaiFeng
Hu, Yunping
Zhang, Yijian
Weng, Hao
Lu, Jianhua
Wang, Xuefeng
Li, Maolan
Liu, Yingbin
Gu, Jun
author_sort Song, Xiaoling
collection PubMed
description Gallbladder cancer (GBC), highly aggressive form of cancer with an extremely poor prognosis, is the most common malignancy of the biliary tract. In this study, we investigated the effects of dioscin (DSN) on human GBC and the potential mechanisms underlying these effects. The results showed that DSN significantly inhibited GBC cell proliferation and migration. Moreover, DSN induced GBC cell apoptosis via mitochondrial dependent apoptotic signalling. Reactive oxygen species (ROS) and glutathione (GSH) levels were measured, and ROS scavengers completely inhibited DSN-induced apoptosis and migration, indicating that ROS play an essential role in GBC progression. Western blot analysis showed that AKT activity was significantly downregulated after DSN treatment, and that inhibition/ectopic expression of AKT enhanced/abolished DSN-induced apoptosis but not migration. Furthermore, we confirmed the relationship between ROS and the PI3K/AKT pathway and found that DSN induced apoptosis by regulating ROS-mediated PI3K/AKT signaling. Taken together, these findings indicate that DSN induces GBC apoptosis through inhibiting ROS-mediated PI3K/AKT signalling.
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spelling pubmed-54856332017-06-27 Dioscin Induces Gallbladder Cancer Apoptosis by Inhibiting ROS-Mediated PI3K/AKT Signalling Song, Xiaoling Wang, Zheng Liang, Haibin Zhang, Wenjie Ye, Yuanyuan Li, HuaiFeng Hu, Yunping Zhang, Yijian Weng, Hao Lu, Jianhua Wang, Xuefeng Li, Maolan Liu, Yingbin Gu, Jun Int J Biol Sci Research Paper Gallbladder cancer (GBC), highly aggressive form of cancer with an extremely poor prognosis, is the most common malignancy of the biliary tract. In this study, we investigated the effects of dioscin (DSN) on human GBC and the potential mechanisms underlying these effects. The results showed that DSN significantly inhibited GBC cell proliferation and migration. Moreover, DSN induced GBC cell apoptosis via mitochondrial dependent apoptotic signalling. Reactive oxygen species (ROS) and glutathione (GSH) levels were measured, and ROS scavengers completely inhibited DSN-induced apoptosis and migration, indicating that ROS play an essential role in GBC progression. Western blot analysis showed that AKT activity was significantly downregulated after DSN treatment, and that inhibition/ectopic expression of AKT enhanced/abolished DSN-induced apoptosis but not migration. Furthermore, we confirmed the relationship between ROS and the PI3K/AKT pathway and found that DSN induced apoptosis by regulating ROS-mediated PI3K/AKT signaling. Taken together, these findings indicate that DSN induces GBC apoptosis through inhibiting ROS-mediated PI3K/AKT signalling. Ivyspring International Publisher 2017-06-01 /pmc/articles/PMC5485633/ /pubmed/28656003 http://dx.doi.org/10.7150/ijbs.18732 Text en © Ivyspring International Publisher This is an open access article distributed under the terms of the Creative Commons Attribution (CC BY-NC) license (https://creativecommons.org/licenses/by-nc/4.0/). See http://ivyspring.com/terms for full terms and conditions.
spellingShingle Research Paper
Song, Xiaoling
Wang, Zheng
Liang, Haibin
Zhang, Wenjie
Ye, Yuanyuan
Li, HuaiFeng
Hu, Yunping
Zhang, Yijian
Weng, Hao
Lu, Jianhua
Wang, Xuefeng
Li, Maolan
Liu, Yingbin
Gu, Jun
Dioscin Induces Gallbladder Cancer Apoptosis by Inhibiting ROS-Mediated PI3K/AKT Signalling
title Dioscin Induces Gallbladder Cancer Apoptosis by Inhibiting ROS-Mediated PI3K/AKT Signalling
title_full Dioscin Induces Gallbladder Cancer Apoptosis by Inhibiting ROS-Mediated PI3K/AKT Signalling
title_fullStr Dioscin Induces Gallbladder Cancer Apoptosis by Inhibiting ROS-Mediated PI3K/AKT Signalling
title_full_unstemmed Dioscin Induces Gallbladder Cancer Apoptosis by Inhibiting ROS-Mediated PI3K/AKT Signalling
title_short Dioscin Induces Gallbladder Cancer Apoptosis by Inhibiting ROS-Mediated PI3K/AKT Signalling
title_sort dioscin induces gallbladder cancer apoptosis by inhibiting ros-mediated pi3k/akt signalling
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5485633/
https://www.ncbi.nlm.nih.gov/pubmed/28656003
http://dx.doi.org/10.7150/ijbs.18732
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