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Protein ubiquitination and formation of polyubiquitin chains without ATP, E1 and E2 enzymes

Studying protein ubiquitination is difficult due to the complexity of the E1–E2–E3 ubiquitination cascade. Here we report the discovery that C-terminal ubiquitin thioesters can undergo direct transthiolation with the catalytic cysteine of the model HECT E3 ubiquitin ligase Rsp5 to form a catalytical...

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Detalles Bibliográficos
Autores principales:	Park, Sungjin, Krist, David T., Statsyuk, Alexander V.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Royal Society of Chemistry 2015
Materias:	Chemistry
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5485889/ https://www.ncbi.nlm.nih.gov/pubmed/28706640 http://dx.doi.org/10.1039/c4sc02340d

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