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NANOG Plays a Hierarchical Role in the Transcription Network Regulating the Pluripotency and Plasticity of Adipose Tissue-Derived Stem Cells

The stromal vascular cell fraction (SVF) of visceral and subcutaneous adipose tissue (VAT and SAT) has increasingly come into focus in stem cell research, since these compartments represent a rich source of multipotent adipose-derived stem cells (ASCs). ASCs exhibit a self-renewal potential and diff...

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Autores principales: Pitrone, Maria, Pizzolanti, Giuseppe, Tomasello, Laura, Coppola, Antonina, Morini, Lorenzo, Pantuso, Gianni, Ficarella, Romina, Guarnotta, Valentina, Perrini, Sebastio, Giorgino, Francesco, Giordano, Carla
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5485931/
https://www.ncbi.nlm.nih.gov/pubmed/28545230
http://dx.doi.org/10.3390/ijms18061107
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author Pitrone, Maria
Pizzolanti, Giuseppe
Tomasello, Laura
Coppola, Antonina
Morini, Lorenzo
Pantuso, Gianni
Ficarella, Romina
Guarnotta, Valentina
Perrini, Sebastio
Giorgino, Francesco
Giordano, Carla
author_facet Pitrone, Maria
Pizzolanti, Giuseppe
Tomasello, Laura
Coppola, Antonina
Morini, Lorenzo
Pantuso, Gianni
Ficarella, Romina
Guarnotta, Valentina
Perrini, Sebastio
Giorgino, Francesco
Giordano, Carla
author_sort Pitrone, Maria
collection PubMed
description The stromal vascular cell fraction (SVF) of visceral and subcutaneous adipose tissue (VAT and SAT) has increasingly come into focus in stem cell research, since these compartments represent a rich source of multipotent adipose-derived stem cells (ASCs). ASCs exhibit a self-renewal potential and differentiation capacity. Our aim was to study the different expression of the embryonic stem cell markers NANOG (homeobox protein NANOG), SOX2 (SRY (sex determining region Y)-box 2) and OCT4 (octamer-binding transcription factor 4) and to evaluate if there exists a hierarchal role in this network in ASCs derived from both SAT and VAT. ASCs were isolated from SAT and VAT biopsies of 72 consenting patients (23 men, 47 women; age 45 ± 10; BMI between 25 ± 5 and 30 ± 5 range) undergoing elective open-abdominal surgery. Sphere-forming capability was evaluated by plating cells in low adhesion plastic. Stem cell markers CD90, CD105, CD29, CD31, CD45 and CD146 were analyzed by flow cytometry, and the stem cell transcription factors NANOG, SOX2 and OCT4 were detected by immunoblotting and real-time PCR. NANOG, SOX2 and OCT4 interplay was explored by gene silencing. ASCs from VAT and SAT confirmed their mesenchymal stem cell (MSC) phenotype expressing the specific MSC markers CD90, CD105, NANOG, SOX2 and OCT4. NANOG silencing induced a significant OCT4 (70 ± 0.05%) and SOX2 (75 ± 0.03%) downregulation, whereas SOX2 silencing did not affect NANOG gene expression. Adipose tissue is an important source of MSC, and siRNA experiments endorse a hierarchical role of NANOG in the complex transcription network that regulates pluripotency.
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spelling pubmed-54859312017-06-29 NANOG Plays a Hierarchical Role in the Transcription Network Regulating the Pluripotency and Plasticity of Adipose Tissue-Derived Stem Cells Pitrone, Maria Pizzolanti, Giuseppe Tomasello, Laura Coppola, Antonina Morini, Lorenzo Pantuso, Gianni Ficarella, Romina Guarnotta, Valentina Perrini, Sebastio Giorgino, Francesco Giordano, Carla Int J Mol Sci Article The stromal vascular cell fraction (SVF) of visceral and subcutaneous adipose tissue (VAT and SAT) has increasingly come into focus in stem cell research, since these compartments represent a rich source of multipotent adipose-derived stem cells (ASCs). ASCs exhibit a self-renewal potential and differentiation capacity. Our aim was to study the different expression of the embryonic stem cell markers NANOG (homeobox protein NANOG), SOX2 (SRY (sex determining region Y)-box 2) and OCT4 (octamer-binding transcription factor 4) and to evaluate if there exists a hierarchal role in this network in ASCs derived from both SAT and VAT. ASCs were isolated from SAT and VAT biopsies of 72 consenting patients (23 men, 47 women; age 45 ± 10; BMI between 25 ± 5 and 30 ± 5 range) undergoing elective open-abdominal surgery. Sphere-forming capability was evaluated by plating cells in low adhesion plastic. Stem cell markers CD90, CD105, CD29, CD31, CD45 and CD146 were analyzed by flow cytometry, and the stem cell transcription factors NANOG, SOX2 and OCT4 were detected by immunoblotting and real-time PCR. NANOG, SOX2 and OCT4 interplay was explored by gene silencing. ASCs from VAT and SAT confirmed their mesenchymal stem cell (MSC) phenotype expressing the specific MSC markers CD90, CD105, NANOG, SOX2 and OCT4. NANOG silencing induced a significant OCT4 (70 ± 0.05%) and SOX2 (75 ± 0.03%) downregulation, whereas SOX2 silencing did not affect NANOG gene expression. Adipose tissue is an important source of MSC, and siRNA experiments endorse a hierarchical role of NANOG in the complex transcription network that regulates pluripotency. MDPI 2017-05-23 /pmc/articles/PMC5485931/ /pubmed/28545230 http://dx.doi.org/10.3390/ijms18061107 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pitrone, Maria
Pizzolanti, Giuseppe
Tomasello, Laura
Coppola, Antonina
Morini, Lorenzo
Pantuso, Gianni
Ficarella, Romina
Guarnotta, Valentina
Perrini, Sebastio
Giorgino, Francesco
Giordano, Carla
NANOG Plays a Hierarchical Role in the Transcription Network Regulating the Pluripotency and Plasticity of Adipose Tissue-Derived Stem Cells
title NANOG Plays a Hierarchical Role in the Transcription Network Regulating the Pluripotency and Plasticity of Adipose Tissue-Derived Stem Cells
title_full NANOG Plays a Hierarchical Role in the Transcription Network Regulating the Pluripotency and Plasticity of Adipose Tissue-Derived Stem Cells
title_fullStr NANOG Plays a Hierarchical Role in the Transcription Network Regulating the Pluripotency and Plasticity of Adipose Tissue-Derived Stem Cells
title_full_unstemmed NANOG Plays a Hierarchical Role in the Transcription Network Regulating the Pluripotency and Plasticity of Adipose Tissue-Derived Stem Cells
title_short NANOG Plays a Hierarchical Role in the Transcription Network Regulating the Pluripotency and Plasticity of Adipose Tissue-Derived Stem Cells
title_sort nanog plays a hierarchical role in the transcription network regulating the pluripotency and plasticity of adipose tissue-derived stem cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5485931/
https://www.ncbi.nlm.nih.gov/pubmed/28545230
http://dx.doi.org/10.3390/ijms18061107
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