The Methods of Choice for Extracellular Vesicles (EVs) Characterization

In recent years, extracellular vesicles (EVs) have become a subject of intense study. These membrane-enclosed spherical structures are secreted by almost every cell type and are engaged in the transport of cellular content (cargo) from parental to target cells. The impact of EVs transfer has been ob...

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Autores principales: Szatanek, Rafał, Baj-Krzyworzeka, Monika, Zimoch, Jakub, Lekka, Małgorzata, Siedlar, Maciej, Baran, Jarek
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5485977/
https://www.ncbi.nlm.nih.gov/pubmed/28555055
http://dx.doi.org/10.3390/ijms18061153
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author Szatanek, Rafał
Baj-Krzyworzeka, Monika
Zimoch, Jakub
Lekka, Małgorzata
Siedlar, Maciej
Baran, Jarek
author_facet Szatanek, Rafał
Baj-Krzyworzeka, Monika
Zimoch, Jakub
Lekka, Małgorzata
Siedlar, Maciej
Baran, Jarek
author_sort Szatanek, Rafał
collection PubMed
description In recent years, extracellular vesicles (EVs) have become a subject of intense study. These membrane-enclosed spherical structures are secreted by almost every cell type and are engaged in the transport of cellular content (cargo) from parental to target cells. The impact of EVs transfer has been observed in many vital cellular processes including cell-to-cell communication and immune response modulation; thus, a fast and precise characterization of EVs may be relevant for both scientific and diagnostic purposes. In this review, the most popular analytical techniques used in EVs studies are presented with the emphasis on exosomes and microvesicles characterization.
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spelling pubmed-54859772017-06-29 The Methods of Choice for Extracellular Vesicles (EVs) Characterization Szatanek, Rafał Baj-Krzyworzeka, Monika Zimoch, Jakub Lekka, Małgorzata Siedlar, Maciej Baran, Jarek Int J Mol Sci Review In recent years, extracellular vesicles (EVs) have become a subject of intense study. These membrane-enclosed spherical structures are secreted by almost every cell type and are engaged in the transport of cellular content (cargo) from parental to target cells. The impact of EVs transfer has been observed in many vital cellular processes including cell-to-cell communication and immune response modulation; thus, a fast and precise characterization of EVs may be relevant for both scientific and diagnostic purposes. In this review, the most popular analytical techniques used in EVs studies are presented with the emphasis on exosomes and microvesicles characterization. MDPI 2017-05-29 /pmc/articles/PMC5485977/ /pubmed/28555055 http://dx.doi.org/10.3390/ijms18061153 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Szatanek, Rafał
Baj-Krzyworzeka, Monika
Zimoch, Jakub
Lekka, Małgorzata
Siedlar, Maciej
Baran, Jarek
The Methods of Choice for Extracellular Vesicles (EVs) Characterization
title The Methods of Choice for Extracellular Vesicles (EVs) Characterization
title_full The Methods of Choice for Extracellular Vesicles (EVs) Characterization
title_fullStr The Methods of Choice for Extracellular Vesicles (EVs) Characterization
title_full_unstemmed The Methods of Choice for Extracellular Vesicles (EVs) Characterization
title_short The Methods of Choice for Extracellular Vesicles (EVs) Characterization
title_sort methods of choice for extracellular vesicles (evs) characterization
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5485977/
https://www.ncbi.nlm.nih.gov/pubmed/28555055
http://dx.doi.org/10.3390/ijms18061153
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