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De Novo Transcriptome Sequencing and the Hypothetical Cold Response Mode of Saussurea involucrata in Extreme Cold Environments

Saussurea involucrata grows in high mountain areas covered by snow throughout the year. The temperature of this habitat can change drastically in one day. To gain a better understanding of the cold response signaling pathways and molecular metabolic reactions involved in cold stress tolerance, genom...

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Autores principales: Li, Jin, Liu, Hailiang, Xia, Wenwen, Mu, Jianqiang, Feng, Yujie, Liu, Ruina, Yan, Panyao, Wang, Aiying, Lin, Zhongping, Guo, Yong, Zhu, Jianbo, Chen, Xianfeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5485979/
https://www.ncbi.nlm.nih.gov/pubmed/28590406
http://dx.doi.org/10.3390/ijms18061155
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author Li, Jin
Liu, Hailiang
Xia, Wenwen
Mu, Jianqiang
Feng, Yujie
Liu, Ruina
Yan, Panyao
Wang, Aiying
Lin, Zhongping
Guo, Yong
Zhu, Jianbo
Chen, Xianfeng
author_facet Li, Jin
Liu, Hailiang
Xia, Wenwen
Mu, Jianqiang
Feng, Yujie
Liu, Ruina
Yan, Panyao
Wang, Aiying
Lin, Zhongping
Guo, Yong
Zhu, Jianbo
Chen, Xianfeng
author_sort Li, Jin
collection PubMed
description Saussurea involucrata grows in high mountain areas covered by snow throughout the year. The temperature of this habitat can change drastically in one day. To gain a better understanding of the cold response signaling pathways and molecular metabolic reactions involved in cold stress tolerance, genome-wide transcriptional analyses were performed using RNA-Seq technologies. A total of 199,758 transcripts were assembled, producing 138,540 unigenes with 46.8 Gb clean data. Overall, 184,416 (92.32%) transcripts were successfully annotated. The 365 transcription factors identified (292 unigenes) belonged to 49 transcription factor families associated with cold stress responses. A total of 343 transcripts on the signal transduction (132 upregulated and 212 downregulated in at least any one of the conditions) were strongly affected by cold temperature, such as the CBL-interacting serine/threonine-protein kinase (CIPKs), receptor-like protein kinases, and protein kinases. The circadian rhythm pathway was activated by cold adaptation, which was necessary to endure the severe temperature changes within a day. There were 346 differentially expressed genes (DEGs) related to transport, of which 138 were upregulated and 22 were downregulated in at least any one of the conditions. Under cold stress conditions, transcriptional regulation, molecular transport, and signal transduction were involved in the adaptation to low temperature in S. involucrata. These findings contribute to our understanding of the adaptation of plants to harsh environments and the survival traits of S. involucrata. In addition, the present study provides insight into the molecular mechanisms of chilling and freezing tolerance.
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spelling pubmed-54859792017-06-29 De Novo Transcriptome Sequencing and the Hypothetical Cold Response Mode of Saussurea involucrata in Extreme Cold Environments Li, Jin Liu, Hailiang Xia, Wenwen Mu, Jianqiang Feng, Yujie Liu, Ruina Yan, Panyao Wang, Aiying Lin, Zhongping Guo, Yong Zhu, Jianbo Chen, Xianfeng Int J Mol Sci Article Saussurea involucrata grows in high mountain areas covered by snow throughout the year. The temperature of this habitat can change drastically in one day. To gain a better understanding of the cold response signaling pathways and molecular metabolic reactions involved in cold stress tolerance, genome-wide transcriptional analyses were performed using RNA-Seq technologies. A total of 199,758 transcripts were assembled, producing 138,540 unigenes with 46.8 Gb clean data. Overall, 184,416 (92.32%) transcripts were successfully annotated. The 365 transcription factors identified (292 unigenes) belonged to 49 transcription factor families associated with cold stress responses. A total of 343 transcripts on the signal transduction (132 upregulated and 212 downregulated in at least any one of the conditions) were strongly affected by cold temperature, such as the CBL-interacting serine/threonine-protein kinase (CIPKs), receptor-like protein kinases, and protein kinases. The circadian rhythm pathway was activated by cold adaptation, which was necessary to endure the severe temperature changes within a day. There were 346 differentially expressed genes (DEGs) related to transport, of which 138 were upregulated and 22 were downregulated in at least any one of the conditions. Under cold stress conditions, transcriptional regulation, molecular transport, and signal transduction were involved in the adaptation to low temperature in S. involucrata. These findings contribute to our understanding of the adaptation of plants to harsh environments and the survival traits of S. involucrata. In addition, the present study provides insight into the molecular mechanisms of chilling and freezing tolerance. MDPI 2017-06-07 /pmc/articles/PMC5485979/ /pubmed/28590406 http://dx.doi.org/10.3390/ijms18061155 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Jin
Liu, Hailiang
Xia, Wenwen
Mu, Jianqiang
Feng, Yujie
Liu, Ruina
Yan, Panyao
Wang, Aiying
Lin, Zhongping
Guo, Yong
Zhu, Jianbo
Chen, Xianfeng
De Novo Transcriptome Sequencing and the Hypothetical Cold Response Mode of Saussurea involucrata in Extreme Cold Environments
title De Novo Transcriptome Sequencing and the Hypothetical Cold Response Mode of Saussurea involucrata in Extreme Cold Environments
title_full De Novo Transcriptome Sequencing and the Hypothetical Cold Response Mode of Saussurea involucrata in Extreme Cold Environments
title_fullStr De Novo Transcriptome Sequencing and the Hypothetical Cold Response Mode of Saussurea involucrata in Extreme Cold Environments
title_full_unstemmed De Novo Transcriptome Sequencing and the Hypothetical Cold Response Mode of Saussurea involucrata in Extreme Cold Environments
title_short De Novo Transcriptome Sequencing and the Hypothetical Cold Response Mode of Saussurea involucrata in Extreme Cold Environments
title_sort de novo transcriptome sequencing and the hypothetical cold response mode of saussurea involucrata in extreme cold environments
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5485979/
https://www.ncbi.nlm.nih.gov/pubmed/28590406
http://dx.doi.org/10.3390/ijms18061155
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