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Stability of BDNF in Human Samples Stored Up to 6 Months and Correlations of Serum and EDTA-Plasma Concentrations
Brain-derived neurotrophic factor (BDNF), an important neural growth factor, has gained growing interest in neuroscience, but many influencing physiological and analytical aspects still remain unclear. In this study we assessed the impact of storage time at room temperature, repeated freeze/thaw cyc...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5486012/ https://www.ncbi.nlm.nih.gov/pubmed/28587193 http://dx.doi.org/10.3390/ijms18061189 |
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author | Polyakova, Maryna Schlögl, Haiko Sacher, Julia Schmidt-Kassow, Maren Kaiser, Jochen Stumvoll, Michael Kratzsch, Jürgen Schroeter, Matthias L. |
author_facet | Polyakova, Maryna Schlögl, Haiko Sacher, Julia Schmidt-Kassow, Maren Kaiser, Jochen Stumvoll, Michael Kratzsch, Jürgen Schroeter, Matthias L. |
author_sort | Polyakova, Maryna |
collection | PubMed |
description | Brain-derived neurotrophic factor (BDNF), an important neural growth factor, has gained growing interest in neuroscience, but many influencing physiological and analytical aspects still remain unclear. In this study we assessed the impact of storage time at room temperature, repeated freeze/thaw cycles, and storage at −80 °C up to 6 months on serum and ethylenediaminetetraacetic acid (EDTA)-plasma BDNF. Furthermore, we assessed correlations of serum and plasma BDNF concentrations in two independent sets of samples. Coefficients of variations (CVs) for serum BDNF concentrations were significantly lower than CVs of plasma concentrations (n = 245, p = 0.006). Mean serum and plasma concentrations at all analyzed time points remained within the acceptable change limit of the inter-assay precision as declared by the manufacturer. Serum and plasma BDNF concentrations correlated positively in both sets of samples and at all analyzed time points of the stability assessment (r = 0.455 to r(s) = 0.596; p < 0.004). In summary, when considering the acceptable change limit, BDNF was stable in serum and in EDTA-plasma up to 6 months. Due to a higher reliability, we suggest favoring serum over EDTA-plasma for future experiments assessing peripheral BDNF concentrations. |
format | Online Article Text |
id | pubmed-5486012 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-54860122017-06-29 Stability of BDNF in Human Samples Stored Up to 6 Months and Correlations of Serum and EDTA-Plasma Concentrations Polyakova, Maryna Schlögl, Haiko Sacher, Julia Schmidt-Kassow, Maren Kaiser, Jochen Stumvoll, Michael Kratzsch, Jürgen Schroeter, Matthias L. Int J Mol Sci Article Brain-derived neurotrophic factor (BDNF), an important neural growth factor, has gained growing interest in neuroscience, but many influencing physiological and analytical aspects still remain unclear. In this study we assessed the impact of storage time at room temperature, repeated freeze/thaw cycles, and storage at −80 °C up to 6 months on serum and ethylenediaminetetraacetic acid (EDTA)-plasma BDNF. Furthermore, we assessed correlations of serum and plasma BDNF concentrations in two independent sets of samples. Coefficients of variations (CVs) for serum BDNF concentrations were significantly lower than CVs of plasma concentrations (n = 245, p = 0.006). Mean serum and plasma concentrations at all analyzed time points remained within the acceptable change limit of the inter-assay precision as declared by the manufacturer. Serum and plasma BDNF concentrations correlated positively in both sets of samples and at all analyzed time points of the stability assessment (r = 0.455 to r(s) = 0.596; p < 0.004). In summary, when considering the acceptable change limit, BDNF was stable in serum and in EDTA-plasma up to 6 months. Due to a higher reliability, we suggest favoring serum over EDTA-plasma for future experiments assessing peripheral BDNF concentrations. MDPI 2017-06-03 /pmc/articles/PMC5486012/ /pubmed/28587193 http://dx.doi.org/10.3390/ijms18061189 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Polyakova, Maryna Schlögl, Haiko Sacher, Julia Schmidt-Kassow, Maren Kaiser, Jochen Stumvoll, Michael Kratzsch, Jürgen Schroeter, Matthias L. Stability of BDNF in Human Samples Stored Up to 6 Months and Correlations of Serum and EDTA-Plasma Concentrations |
title | Stability of BDNF in Human Samples Stored Up to 6 Months and Correlations of Serum and EDTA-Plasma Concentrations |
title_full | Stability of BDNF in Human Samples Stored Up to 6 Months and Correlations of Serum and EDTA-Plasma Concentrations |
title_fullStr | Stability of BDNF in Human Samples Stored Up to 6 Months and Correlations of Serum and EDTA-Plasma Concentrations |
title_full_unstemmed | Stability of BDNF in Human Samples Stored Up to 6 Months and Correlations of Serum and EDTA-Plasma Concentrations |
title_short | Stability of BDNF in Human Samples Stored Up to 6 Months and Correlations of Serum and EDTA-Plasma Concentrations |
title_sort | stability of bdnf in human samples stored up to 6 months and correlations of serum and edta-plasma concentrations |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5486012/ https://www.ncbi.nlm.nih.gov/pubmed/28587193 http://dx.doi.org/10.3390/ijms18061189 |
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