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Quantification of Bone Fatty Acid Metabolism and Its Regulation by Adipocyte Lipoprotein Lipase

Adipocytes are master regulators of energy homeostasis. Although the contributions of classical brown and white adipose tissue (BAT and WAT, respectively) to glucose and fatty acid metabolism are well characterized, the metabolic role of adipocytes in bone marrow remains largely unclear. Here, we qu...

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Autores principales: Bartelt, Alexander, Koehne, Till, Tödter, Klaus, Reimer, Rudolph, Müller, Brigitte, Behler-Janbeck, Friederike, Heeren, Joerg, Scheja, Ludger, Niemeier, Andreas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5486086/
https://www.ncbi.nlm.nih.gov/pubmed/28608812
http://dx.doi.org/10.3390/ijms18061264
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author Bartelt, Alexander
Koehne, Till
Tödter, Klaus
Reimer, Rudolph
Müller, Brigitte
Behler-Janbeck, Friederike
Heeren, Joerg
Scheja, Ludger
Niemeier, Andreas
author_facet Bartelt, Alexander
Koehne, Till
Tödter, Klaus
Reimer, Rudolph
Müller, Brigitte
Behler-Janbeck, Friederike
Heeren, Joerg
Scheja, Ludger
Niemeier, Andreas
author_sort Bartelt, Alexander
collection PubMed
description Adipocytes are master regulators of energy homeostasis. Although the contributions of classical brown and white adipose tissue (BAT and WAT, respectively) to glucose and fatty acid metabolism are well characterized, the metabolic role of adipocytes in bone marrow remains largely unclear. Here, we quantify bone fatty acid metabolism and its contribution to systemic nutrient handling in mice. Whereas in parts of the skeleton the specific amount of nutrients taken-up from the circulation was lower than in other metabolically active tissues such as BAT or liver, the overall contribution of the skeleton as a whole organ was remarkable, placing it among the top organs involved in systemic glucose as well as fatty acid clearance. We show that there are considerable site-specific variations in bone marrow fatty acid composition throughout the skeleton and that, especially in the tibia, marrow fatty acid profiles resemble classical BAT and WAT. Using a mouse model lacking lipoprotein lipase (LPL), a master regulator of plasma lipid turnover specifically in adipocytes, we show that impaired fatty acid flux leads to reduced amounts of dietary essential fatty acids while there was a profound increase in de novo produced fatty acids in both bone marrow and cortical bone. Notably, these changes in fatty acid profiles were not associated with any gross skeletal phenotype. These results identify LPL as an important regulator of fatty acid transport to skeletal compartments and demonstrate an intricate functional link between systemic and skeletal fatty acid and glucose metabolism.
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spelling pubmed-54860862017-06-29 Quantification of Bone Fatty Acid Metabolism and Its Regulation by Adipocyte Lipoprotein Lipase Bartelt, Alexander Koehne, Till Tödter, Klaus Reimer, Rudolph Müller, Brigitte Behler-Janbeck, Friederike Heeren, Joerg Scheja, Ludger Niemeier, Andreas Int J Mol Sci Article Adipocytes are master regulators of energy homeostasis. Although the contributions of classical brown and white adipose tissue (BAT and WAT, respectively) to glucose and fatty acid metabolism are well characterized, the metabolic role of adipocytes in bone marrow remains largely unclear. Here, we quantify bone fatty acid metabolism and its contribution to systemic nutrient handling in mice. Whereas in parts of the skeleton the specific amount of nutrients taken-up from the circulation was lower than in other metabolically active tissues such as BAT or liver, the overall contribution of the skeleton as a whole organ was remarkable, placing it among the top organs involved in systemic glucose as well as fatty acid clearance. We show that there are considerable site-specific variations in bone marrow fatty acid composition throughout the skeleton and that, especially in the tibia, marrow fatty acid profiles resemble classical BAT and WAT. Using a mouse model lacking lipoprotein lipase (LPL), a master regulator of plasma lipid turnover specifically in adipocytes, we show that impaired fatty acid flux leads to reduced amounts of dietary essential fatty acids while there was a profound increase in de novo produced fatty acids in both bone marrow and cortical bone. Notably, these changes in fatty acid profiles were not associated with any gross skeletal phenotype. These results identify LPL as an important regulator of fatty acid transport to skeletal compartments and demonstrate an intricate functional link between systemic and skeletal fatty acid and glucose metabolism. MDPI 2017-06-13 /pmc/articles/PMC5486086/ /pubmed/28608812 http://dx.doi.org/10.3390/ijms18061264 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bartelt, Alexander
Koehne, Till
Tödter, Klaus
Reimer, Rudolph
Müller, Brigitte
Behler-Janbeck, Friederike
Heeren, Joerg
Scheja, Ludger
Niemeier, Andreas
Quantification of Bone Fatty Acid Metabolism and Its Regulation by Adipocyte Lipoprotein Lipase
title Quantification of Bone Fatty Acid Metabolism and Its Regulation by Adipocyte Lipoprotein Lipase
title_full Quantification of Bone Fatty Acid Metabolism and Its Regulation by Adipocyte Lipoprotein Lipase
title_fullStr Quantification of Bone Fatty Acid Metabolism and Its Regulation by Adipocyte Lipoprotein Lipase
title_full_unstemmed Quantification of Bone Fatty Acid Metabolism and Its Regulation by Adipocyte Lipoprotein Lipase
title_short Quantification of Bone Fatty Acid Metabolism and Its Regulation by Adipocyte Lipoprotein Lipase
title_sort quantification of bone fatty acid metabolism and its regulation by adipocyte lipoprotein lipase
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5486086/
https://www.ncbi.nlm.nih.gov/pubmed/28608812
http://dx.doi.org/10.3390/ijms18061264
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