Cargando…

Terpenoids from Platostoma rotundifolium (Briq.) A. J. Paton Alter the Expression of Quorum Sensing-Related Virulence Factors and the Formation of Biofilm in Pseudomonas aeruginosa PAO1

Platostoma rotundifolium (Briq.) A. J. Paton aerial parts are widely used in Burundi traditional medicine to treat infectious diseases. In order to investigate their probable antibacterial activities, crude extracts from P. rotundifolium were assessed for their bactericidal and anti-virulence proper...

Descripción completa

Detalles Bibliográficos
Autores principales: Rasamiravaka, Tsiry, Ngezahayo, Jérémie, Pottier, Laurent, Oliveira Ribeiro, Sofia, Souard, Florence, Hari, Léonard, Stévigny, Caroline, El Jaziri, Mondher, Duez, Pierre
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5486092/
https://www.ncbi.nlm.nih.gov/pubmed/28613253
http://dx.doi.org/10.3390/ijms18061270
_version_ 1783246199456792576
author Rasamiravaka, Tsiry
Ngezahayo, Jérémie
Pottier, Laurent
Oliveira Ribeiro, Sofia
Souard, Florence
Hari, Léonard
Stévigny, Caroline
El Jaziri, Mondher
Duez, Pierre
author_facet Rasamiravaka, Tsiry
Ngezahayo, Jérémie
Pottier, Laurent
Oliveira Ribeiro, Sofia
Souard, Florence
Hari, Léonard
Stévigny, Caroline
El Jaziri, Mondher
Duez, Pierre
author_sort Rasamiravaka, Tsiry
collection PubMed
description Platostoma rotundifolium (Briq.) A. J. Paton aerial parts are widely used in Burundi traditional medicine to treat infectious diseases. In order to investigate their probable antibacterial activities, crude extracts from P. rotundifolium were assessed for their bactericidal and anti-virulence properties against an opportunistic bacterial model, Pseudomonas aeruginosa PAO1. Whereas none of the tested extracts exert bacteriostatic and/or bactericidal proprieties, the ethyl acetate and dichloromethane extracts exhibit anti-virulence properties against Pseudomonas aeruginosa PAO1 characterized by an alteration in quorum sensing gene expression and biofilm formation without affecting bacterial viability. Bioguided fractionation of the ethyl acetate extract led to the isolation of major anti-virulence compounds that were identified from nuclear magnetic resonance and high-resolution molecular spectroscopy spectra as cassipourol, β-sitosterol and α-amyrin. Globally, cassipourol and β-sitosterol inhibit quorum sensing-regulated and -regulatory genes expression in las and rhl systems without affecting the global regulators gacA and vfr, whereas α-amyrin had no effect on the expression of these genes. These terpenoids disrupt the formation of biofilms at concentrations down to 12.5, 50 and 50 µM for cassipourol, β-sitosterol and α-amyrin, respectively. Moreover, these terpenoids reduce the production of total exopolysaccharides and promote flagella-dependent motilities (swimming and swarming). The isolated terpenoids exert a wide range of inhibition processes, suggesting a complex mechanism of action targeting P. aeruginosa virulence mechanisms which support the wide anti-infectious use of this plant species in traditional Burundian medicine.
format Online
Article
Text
id pubmed-5486092
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-54860922017-06-29 Terpenoids from Platostoma rotundifolium (Briq.) A. J. Paton Alter the Expression of Quorum Sensing-Related Virulence Factors and the Formation of Biofilm in Pseudomonas aeruginosa PAO1 Rasamiravaka, Tsiry Ngezahayo, Jérémie Pottier, Laurent Oliveira Ribeiro, Sofia Souard, Florence Hari, Léonard Stévigny, Caroline El Jaziri, Mondher Duez, Pierre Int J Mol Sci Article Platostoma rotundifolium (Briq.) A. J. Paton aerial parts are widely used in Burundi traditional medicine to treat infectious diseases. In order to investigate their probable antibacterial activities, crude extracts from P. rotundifolium were assessed for their bactericidal and anti-virulence properties against an opportunistic bacterial model, Pseudomonas aeruginosa PAO1. Whereas none of the tested extracts exert bacteriostatic and/or bactericidal proprieties, the ethyl acetate and dichloromethane extracts exhibit anti-virulence properties against Pseudomonas aeruginosa PAO1 characterized by an alteration in quorum sensing gene expression and biofilm formation without affecting bacterial viability. Bioguided fractionation of the ethyl acetate extract led to the isolation of major anti-virulence compounds that were identified from nuclear magnetic resonance and high-resolution molecular spectroscopy spectra as cassipourol, β-sitosterol and α-amyrin. Globally, cassipourol and β-sitosterol inhibit quorum sensing-regulated and -regulatory genes expression in las and rhl systems without affecting the global regulators gacA and vfr, whereas α-amyrin had no effect on the expression of these genes. These terpenoids disrupt the formation of biofilms at concentrations down to 12.5, 50 and 50 µM for cassipourol, β-sitosterol and α-amyrin, respectively. Moreover, these terpenoids reduce the production of total exopolysaccharides and promote flagella-dependent motilities (swimming and swarming). The isolated terpenoids exert a wide range of inhibition processes, suggesting a complex mechanism of action targeting P. aeruginosa virulence mechanisms which support the wide anti-infectious use of this plant species in traditional Burundian medicine. MDPI 2017-06-14 /pmc/articles/PMC5486092/ /pubmed/28613253 http://dx.doi.org/10.3390/ijms18061270 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rasamiravaka, Tsiry
Ngezahayo, Jérémie
Pottier, Laurent
Oliveira Ribeiro, Sofia
Souard, Florence
Hari, Léonard
Stévigny, Caroline
El Jaziri, Mondher
Duez, Pierre
Terpenoids from Platostoma rotundifolium (Briq.) A. J. Paton Alter the Expression of Quorum Sensing-Related Virulence Factors and the Formation of Biofilm in Pseudomonas aeruginosa PAO1
title Terpenoids from Platostoma rotundifolium (Briq.) A. J. Paton Alter the Expression of Quorum Sensing-Related Virulence Factors and the Formation of Biofilm in Pseudomonas aeruginosa PAO1
title_full Terpenoids from Platostoma rotundifolium (Briq.) A. J. Paton Alter the Expression of Quorum Sensing-Related Virulence Factors and the Formation of Biofilm in Pseudomonas aeruginosa PAO1
title_fullStr Terpenoids from Platostoma rotundifolium (Briq.) A. J. Paton Alter the Expression of Quorum Sensing-Related Virulence Factors and the Formation of Biofilm in Pseudomonas aeruginosa PAO1
title_full_unstemmed Terpenoids from Platostoma rotundifolium (Briq.) A. J. Paton Alter the Expression of Quorum Sensing-Related Virulence Factors and the Formation of Biofilm in Pseudomonas aeruginosa PAO1
title_short Terpenoids from Platostoma rotundifolium (Briq.) A. J. Paton Alter the Expression of Quorum Sensing-Related Virulence Factors and the Formation of Biofilm in Pseudomonas aeruginosa PAO1
title_sort terpenoids from platostoma rotundifolium (briq.) a. j. paton alter the expression of quorum sensing-related virulence factors and the formation of biofilm in pseudomonas aeruginosa pao1
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5486092/
https://www.ncbi.nlm.nih.gov/pubmed/28613253
http://dx.doi.org/10.3390/ijms18061270
work_keys_str_mv AT rasamiravakatsiry terpenoidsfromplatostomarotundifoliumbriqajpatonaltertheexpressionofquorumsensingrelatedvirulencefactorsandtheformationofbiofilminpseudomonasaeruginosapao1
AT ngezahayojeremie terpenoidsfromplatostomarotundifoliumbriqajpatonaltertheexpressionofquorumsensingrelatedvirulencefactorsandtheformationofbiofilminpseudomonasaeruginosapao1
AT pottierlaurent terpenoidsfromplatostomarotundifoliumbriqajpatonaltertheexpressionofquorumsensingrelatedvirulencefactorsandtheformationofbiofilminpseudomonasaeruginosapao1
AT oliveiraribeirosofia terpenoidsfromplatostomarotundifoliumbriqajpatonaltertheexpressionofquorumsensingrelatedvirulencefactorsandtheformationofbiofilminpseudomonasaeruginosapao1
AT souardflorence terpenoidsfromplatostomarotundifoliumbriqajpatonaltertheexpressionofquorumsensingrelatedvirulencefactorsandtheformationofbiofilminpseudomonasaeruginosapao1
AT harileonard terpenoidsfromplatostomarotundifoliumbriqajpatonaltertheexpressionofquorumsensingrelatedvirulencefactorsandtheformationofbiofilminpseudomonasaeruginosapao1
AT stevignycaroline terpenoidsfromplatostomarotundifoliumbriqajpatonaltertheexpressionofquorumsensingrelatedvirulencefactorsandtheformationofbiofilminpseudomonasaeruginosapao1
AT eljazirimondher terpenoidsfromplatostomarotundifoliumbriqajpatonaltertheexpressionofquorumsensingrelatedvirulencefactorsandtheformationofbiofilminpseudomonasaeruginosapao1
AT duezpierre terpenoidsfromplatostomarotundifoliumbriqajpatonaltertheexpressionofquorumsensingrelatedvirulencefactorsandtheformationofbiofilminpseudomonasaeruginosapao1