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The Influence of Quercetin on Maternal Immunity, Oxidative Stress, and Inflammation in Mice with Exposure of Fine Particulate Matter during Gestation

The objective is to investigate the influence of PM(2.5) exposure on peripheral blood lymphocyte subsets in pregnant mice and the antagonism of quercetin on adverse effects induced by PM(2.5) exposure. Pregnant mice were randomly divided into control group, PM(2.5) model group and 3 quercetin interv...

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Autores principales: Liu, Wei, Zhang, Minjia, Feng, Jinqiu, Fan, Aiqin, Zhou, Yalin, Xu, Yajun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5486278/
https://www.ncbi.nlm.nih.gov/pubmed/28574437
http://dx.doi.org/10.3390/ijerph14060592
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author Liu, Wei
Zhang, Minjia
Feng, Jinqiu
Fan, Aiqin
Zhou, Yalin
Xu, Yajun
author_facet Liu, Wei
Zhang, Minjia
Feng, Jinqiu
Fan, Aiqin
Zhou, Yalin
Xu, Yajun
author_sort Liu, Wei
collection PubMed
description The objective is to investigate the influence of PM(2.5) exposure on peripheral blood lymphocyte subsets in pregnant mice and the antagonism of quercetin on adverse effects induced by PM(2.5) exposure. Pregnant mice were randomly divided into control group, PM(2.5) model group and 3 quercetin intervention groups. Dams in all groups except the control group were exposed to PM(2.5) suspension by intratracheal instillation on gestational day (GD) 3, 6, 9, 12 and 15. Meanwhile, each dam was given 0.15% carboxymethylcellulose sodium (CMCS) (control group & PM(2.5) model group) and different doses of quercetin (quercetin intervention groups) by gavage once a day from GD0 to GD17. The percentage of lymphocyte subsets, Biomarkers of systemic inflammation injuries (IL-2, IL-6, IL-8 & TNF-α) and oxidative stress indicators (CAT, GSH & HO-1) in peripheral blood of the dams were analyzed. The number of T cells increased, accompanied by increased level of IL-2, IL-6, IL-8 and HO-1 due to PM(2.5) exposure. Less CD4+ and CD8+ T cells were counted in 100 mg/kg quercetin intervention group, compared with PM(2.5) model group. Quercetin may inhibit cytokine production, especially in IL-6 and IL-8 and may upgrade the level of HO-1. Our findings indicate that PM(2.5) could significantly influence the distribution of T-lymphocyte subsets, activate inflammatory reaction and elevate oxidative stress level in peripheral blood of pregnant mice. Certain dose of quercetin administration during pregnancy may protect the dams against the adverse effects through various ways.
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spelling pubmed-54862782017-06-30 The Influence of Quercetin on Maternal Immunity, Oxidative Stress, and Inflammation in Mice with Exposure of Fine Particulate Matter during Gestation Liu, Wei Zhang, Minjia Feng, Jinqiu Fan, Aiqin Zhou, Yalin Xu, Yajun Int J Environ Res Public Health Article The objective is to investigate the influence of PM(2.5) exposure on peripheral blood lymphocyte subsets in pregnant mice and the antagonism of quercetin on adverse effects induced by PM(2.5) exposure. Pregnant mice were randomly divided into control group, PM(2.5) model group and 3 quercetin intervention groups. Dams in all groups except the control group were exposed to PM(2.5) suspension by intratracheal instillation on gestational day (GD) 3, 6, 9, 12 and 15. Meanwhile, each dam was given 0.15% carboxymethylcellulose sodium (CMCS) (control group & PM(2.5) model group) and different doses of quercetin (quercetin intervention groups) by gavage once a day from GD0 to GD17. The percentage of lymphocyte subsets, Biomarkers of systemic inflammation injuries (IL-2, IL-6, IL-8 & TNF-α) and oxidative stress indicators (CAT, GSH & HO-1) in peripheral blood of the dams were analyzed. The number of T cells increased, accompanied by increased level of IL-2, IL-6, IL-8 and HO-1 due to PM(2.5) exposure. Less CD4+ and CD8+ T cells were counted in 100 mg/kg quercetin intervention group, compared with PM(2.5) model group. Quercetin may inhibit cytokine production, especially in IL-6 and IL-8 and may upgrade the level of HO-1. Our findings indicate that PM(2.5) could significantly influence the distribution of T-lymphocyte subsets, activate inflammatory reaction and elevate oxidative stress level in peripheral blood of pregnant mice. Certain dose of quercetin administration during pregnancy may protect the dams against the adverse effects through various ways. MDPI 2017-06-02 2017-06 /pmc/articles/PMC5486278/ /pubmed/28574437 http://dx.doi.org/10.3390/ijerph14060592 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Wei
Zhang, Minjia
Feng, Jinqiu
Fan, Aiqin
Zhou, Yalin
Xu, Yajun
The Influence of Quercetin on Maternal Immunity, Oxidative Stress, and Inflammation in Mice with Exposure of Fine Particulate Matter during Gestation
title The Influence of Quercetin on Maternal Immunity, Oxidative Stress, and Inflammation in Mice with Exposure of Fine Particulate Matter during Gestation
title_full The Influence of Quercetin on Maternal Immunity, Oxidative Stress, and Inflammation in Mice with Exposure of Fine Particulate Matter during Gestation
title_fullStr The Influence of Quercetin on Maternal Immunity, Oxidative Stress, and Inflammation in Mice with Exposure of Fine Particulate Matter during Gestation
title_full_unstemmed The Influence of Quercetin on Maternal Immunity, Oxidative Stress, and Inflammation in Mice with Exposure of Fine Particulate Matter during Gestation
title_short The Influence of Quercetin on Maternal Immunity, Oxidative Stress, and Inflammation in Mice with Exposure of Fine Particulate Matter during Gestation
title_sort influence of quercetin on maternal immunity, oxidative stress, and inflammation in mice with exposure of fine particulate matter during gestation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5486278/
https://www.ncbi.nlm.nih.gov/pubmed/28574437
http://dx.doi.org/10.3390/ijerph14060592
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