Development of a Single-Drop Microextraction with Derivatization Procedure for Analysis of Volatile Fatty Acids in Water Samples

A single-drop microextraction (SDME) was developed for the analysis of volatile fatty acids (VFAs) (C(2)–C(7)) in water by gas chromatography (GC) with flame ionization detection. The significant parameters affecting the SDME performance such as selection of microextraction solvent, extraction time, stirring rate, sample pH and temperature, and ionic strength were studied and optimized. To lower limits of detection, derivatization of VFAs by N-methyl-N-(tert-butyldimethylsilyl)trifluoroacetamide (MTBSTFA) was performed. The method developed requires very short time of extraction and derivatization (13 min) and it is characterized by a good precision (max RSD = 11.4%), linearity and relatively low limits of detection (from 8.3 mg L(−1) for acetic acid to 0.008 mg L(−1) for heptanoic acid). The results of the SDME in combination with GC show promising potential for the analysis of VFAs in water samples.