Metabolic response of porcine colon explants to in vitro infection by Brachyspira hyodysenteriae: a leap into disease pathophysiology

INTRODUCTION: Swine dysentery caused by Brachyspira hyodysenteriae is a production limiting disease in pig farming. Currently antimicrobial therapy is the only treatment and control method available. OBJECTIVE: The aim of this study was to characterize the metabolic response of porcine colon explants to infection by B. hyodysenteriae. METHODS: Porcine colon explants exposed to B. hyodysenteriae were analyzed for histopathological, metabolic and pro-inflammatory gene expression changes. RESULTS: Significant epithelial necrosis, increased levels of l-citrulline and IL-1α were observed on explants infected with B. hyodysenteriae. CONCLUSIONS: The spirochete induces necrosis in vitro likely through an inflammatory process mediated by IL-1α and NO. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11306-017-1219-6) contains supplementary material, which is available to authorized users.