Cargando…

Antifeedants Produced by Bacteria Associated with the Gut of the Pine Weevil Hylobius abietis

The pine weevil, Hylobius abietis, is a severe forest pest insect as it feeds on newly planted conifer seedlings. To identify and develop an antifeedant could be one step towards the protection of seedlings from feeding damage by the pine weevil. With the aim to trace the origin of the antifeedants...

Descripción completa

Detalles Bibliográficos
Autores principales: Axelsson, Karolin, Konstanzer, Vera, Rajarao, Gunaratna Kuttuva, Terenius, Olle, Seriot, Lisa, Nordenhem, Henrik, Nordlander, Göran, Borg-Karlson, Anna-Karin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2017
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5486832/
https://www.ncbi.nlm.nih.gov/pubmed/28074245
http://dx.doi.org/10.1007/s00248-016-0915-5
_version_ 1783246341379457024
author Axelsson, Karolin
Konstanzer, Vera
Rajarao, Gunaratna Kuttuva
Terenius, Olle
Seriot, Lisa
Nordenhem, Henrik
Nordlander, Göran
Borg-Karlson, Anna-Karin
author_facet Axelsson, Karolin
Konstanzer, Vera
Rajarao, Gunaratna Kuttuva
Terenius, Olle
Seriot, Lisa
Nordenhem, Henrik
Nordlander, Göran
Borg-Karlson, Anna-Karin
author_sort Axelsson, Karolin
collection PubMed
description The pine weevil, Hylobius abietis, is a severe forest pest insect as it feeds on newly planted conifer seedlings. To identify and develop an antifeedant could be one step towards the protection of seedlings from feeding damage by the pine weevil. With the aim to trace the origin of the antifeedants previously found in feces of the pine weevil, we investigated the culturable bacteria associated with the gut and identified the volatiles they produced. Bacterial isolates were identified by 16S ribosomal RNA gene analysis. The volatile emissions of selected bacteria, cultivated on NB media or on the grated phloem of Scots pine twigs dispersed in water, were collected and analyzed by solid-phase microextraction gas chromatography-mass spectrometry. The bacterial isolates released a variety of compounds, among others 2-methoxyphenol, 2-phenylethanol, 3-methyl-1-butanol, 1-octen-3-ol, 3-octanone, dimethyl disulfide, and dimethyl trisulfide. A strong antifeedant effect was observed by 2-phenylethanol, which could thus be a good candidate for use to protect planted conifer seedlings against feeding damage caused by H. abietis.
format Online
Article
Text
id pubmed-5486832
institution National Center for Biotechnology Information
language English
publishDate 2017
publisher Springer US
record_format MEDLINE/PubMed
spelling pubmed-54868322017-07-11 Antifeedants Produced by Bacteria Associated with the Gut of the Pine Weevil Hylobius abietis Axelsson, Karolin Konstanzer, Vera Rajarao, Gunaratna Kuttuva Terenius, Olle Seriot, Lisa Nordenhem, Henrik Nordlander, Göran Borg-Karlson, Anna-Karin Microb Ecol Invertebrate Microbiology The pine weevil, Hylobius abietis, is a severe forest pest insect as it feeds on newly planted conifer seedlings. To identify and develop an antifeedant could be one step towards the protection of seedlings from feeding damage by the pine weevil. With the aim to trace the origin of the antifeedants previously found in feces of the pine weevil, we investigated the culturable bacteria associated with the gut and identified the volatiles they produced. Bacterial isolates were identified by 16S ribosomal RNA gene analysis. The volatile emissions of selected bacteria, cultivated on NB media or on the grated phloem of Scots pine twigs dispersed in water, were collected and analyzed by solid-phase microextraction gas chromatography-mass spectrometry. The bacterial isolates released a variety of compounds, among others 2-methoxyphenol, 2-phenylethanol, 3-methyl-1-butanol, 1-octen-3-ol, 3-octanone, dimethyl disulfide, and dimethyl trisulfide. A strong antifeedant effect was observed by 2-phenylethanol, which could thus be a good candidate for use to protect planted conifer seedlings against feeding damage caused by H. abietis. Springer US 2017-01-10 2017 /pmc/articles/PMC5486832/ /pubmed/28074245 http://dx.doi.org/10.1007/s00248-016-0915-5 Text en © The Author(s) 2017 Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Invertebrate Microbiology
Axelsson, Karolin
Konstanzer, Vera
Rajarao, Gunaratna Kuttuva
Terenius, Olle
Seriot, Lisa
Nordenhem, Henrik
Nordlander, Göran
Borg-Karlson, Anna-Karin
Antifeedants Produced by Bacteria Associated with the Gut of the Pine Weevil Hylobius abietis
title Antifeedants Produced by Bacteria Associated with the Gut of the Pine Weevil Hylobius abietis
title_full Antifeedants Produced by Bacteria Associated with the Gut of the Pine Weevil Hylobius abietis
title_fullStr Antifeedants Produced by Bacteria Associated with the Gut of the Pine Weevil Hylobius abietis
title_full_unstemmed Antifeedants Produced by Bacteria Associated with the Gut of the Pine Weevil Hylobius abietis
title_short Antifeedants Produced by Bacteria Associated with the Gut of the Pine Weevil Hylobius abietis
title_sort antifeedants produced by bacteria associated with the gut of the pine weevil hylobius abietis
topic Invertebrate Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5486832/
https://www.ncbi.nlm.nih.gov/pubmed/28074245
http://dx.doi.org/10.1007/s00248-016-0915-5
work_keys_str_mv AT axelssonkarolin antifeedantsproducedbybacteriaassociatedwiththegutofthepineweevilhylobiusabietis
AT konstanzervera antifeedantsproducedbybacteriaassociatedwiththegutofthepineweevilhylobiusabietis
AT rajaraogunaratnakuttuva antifeedantsproducedbybacteriaassociatedwiththegutofthepineweevilhylobiusabietis
AT tereniusolle antifeedantsproducedbybacteriaassociatedwiththegutofthepineweevilhylobiusabietis
AT seriotlisa antifeedantsproducedbybacteriaassociatedwiththegutofthepineweevilhylobiusabietis
AT nordenhemhenrik antifeedantsproducedbybacteriaassociatedwiththegutofthepineweevilhylobiusabietis
AT nordlandergoran antifeedantsproducedbybacteriaassociatedwiththegutofthepineweevilhylobiusabietis
AT borgkarlsonannakarin antifeedantsproducedbybacteriaassociatedwiththegutofthepineweevilhylobiusabietis